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Prevalence of Multidrug-Resistant Enterococcus faecalis in Hospital-Acquired Surgical Wound Infections and Bacteremia: Concomitant Analysis of Antimicrobial Resistance Genes
BACKGROUND: The study aimed to assess the prevalence of Enterococcus faecalis infections among patients with hospital-acquired surgical wound sepsis and bacteremia in surgical wards and identify the antimicrobial susceptibility in these pathogens. Genetic role of erythromycin, vancomycin, and cephal...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6796195/ https://www.ncbi.nlm.nih.gov/pubmed/31662606 http://dx.doi.org/10.1177/1178633719882929 |
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author | Esmail, Mona Abdel Monem Abdulghany, Hend M Khairy, Rasha MM |
author_facet | Esmail, Mona Abdel Monem Abdulghany, Hend M Khairy, Rasha MM |
author_sort | Esmail, Mona Abdel Monem |
collection | PubMed |
description | BACKGROUND: The study aimed to assess the prevalence of Enterococcus faecalis infections among patients with hospital-acquired surgical wound sepsis and bacteremia in surgical wards and identify the antimicrobial susceptibility in these pathogens. Genetic role of erythromycin, vancomycin, and cephalosporin resistance in these pathogens was also examined. METHODS: Two hundred samples were collected from surgical wound infections and 100 blood cultures from patients with suggested bacteremia to identify E faecalis by phenotypic and genotypic methods. Antimicrobial susceptibility to 12 antimicrobial agents was tested. The presence of resistance genes was examined by polymerase chain reaction (PCR) assay. RESULTS: E faecalis was isolated with a frequency of 24/200 (12%) from surgical wound samples and 2/100 (2%) from blood cultures. All isolates were completely resistant to cefepime, ampicillin, and tetracycline, 96% of isolates were resistant to erythromycin, 53.8% to vancomycin, and 23.1% to linezolid. Multidrug resistance (MDR) was found in 100% of isolates. ere(B) and erm(B) genes were present in 20/25 (80%) and 17/25 (68%) of erythromycin-resistant isolates, respectively, 15 (60%) isolates carry both ere(B) and erm(B) genes. Van A gene was detected in 71.4% of vancomycin-resistant isolates. All isolates were negative for mef(A/E), blaSHV, and blaTEM genes. CONCLUSION: MDR in all isolates (100%) and high-level resistance to gentamicin, erythromycin, and vancomycin were reported in E Faecalis isolates. In the studied isolates, erythromycin resistance mainly related to the presence of ere(B) and erm(B) genes and vancomycin resistance was mainly related to the presence of vanA gene. |
format | Online Article Text |
id | pubmed-6796195 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-67961952019-10-29 Prevalence of Multidrug-Resistant Enterococcus faecalis in Hospital-Acquired Surgical Wound Infections and Bacteremia: Concomitant Analysis of Antimicrobial Resistance Genes Esmail, Mona Abdel Monem Abdulghany, Hend M Khairy, Rasha MM Infect Dis (Auckl) IDR-9 Antimicrobial Resistance BACKGROUND: The study aimed to assess the prevalence of Enterococcus faecalis infections among patients with hospital-acquired surgical wound sepsis and bacteremia in surgical wards and identify the antimicrobial susceptibility in these pathogens. Genetic role of erythromycin, vancomycin, and cephalosporin resistance in these pathogens was also examined. METHODS: Two hundred samples were collected from surgical wound infections and 100 blood cultures from patients with suggested bacteremia to identify E faecalis by phenotypic and genotypic methods. Antimicrobial susceptibility to 12 antimicrobial agents was tested. The presence of resistance genes was examined by polymerase chain reaction (PCR) assay. RESULTS: E faecalis was isolated with a frequency of 24/200 (12%) from surgical wound samples and 2/100 (2%) from blood cultures. All isolates were completely resistant to cefepime, ampicillin, and tetracycline, 96% of isolates were resistant to erythromycin, 53.8% to vancomycin, and 23.1% to linezolid. Multidrug resistance (MDR) was found in 100% of isolates. ere(B) and erm(B) genes were present in 20/25 (80%) and 17/25 (68%) of erythromycin-resistant isolates, respectively, 15 (60%) isolates carry both ere(B) and erm(B) genes. Van A gene was detected in 71.4% of vancomycin-resistant isolates. All isolates were negative for mef(A/E), blaSHV, and blaTEM genes. CONCLUSION: MDR in all isolates (100%) and high-level resistance to gentamicin, erythromycin, and vancomycin were reported in E Faecalis isolates. In the studied isolates, erythromycin resistance mainly related to the presence of ere(B) and erm(B) genes and vancomycin resistance was mainly related to the presence of vanA gene. SAGE Publications 2019-10-15 /pmc/articles/PMC6796195/ /pubmed/31662606 http://dx.doi.org/10.1177/1178633719882929 Text en © The Author(s) 2019 http://www.creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | IDR-9 Antimicrobial Resistance Esmail, Mona Abdel Monem Abdulghany, Hend M Khairy, Rasha MM Prevalence of Multidrug-Resistant Enterococcus faecalis in Hospital-Acquired Surgical Wound Infections and Bacteremia: Concomitant Analysis of Antimicrobial Resistance Genes |
title | Prevalence of Multidrug-Resistant Enterococcus
faecalis in Hospital-Acquired Surgical Wound Infections and
Bacteremia: Concomitant Analysis of Antimicrobial Resistance
Genes |
title_full | Prevalence of Multidrug-Resistant Enterococcus
faecalis in Hospital-Acquired Surgical Wound Infections and
Bacteremia: Concomitant Analysis of Antimicrobial Resistance
Genes |
title_fullStr | Prevalence of Multidrug-Resistant Enterococcus
faecalis in Hospital-Acquired Surgical Wound Infections and
Bacteremia: Concomitant Analysis of Antimicrobial Resistance
Genes |
title_full_unstemmed | Prevalence of Multidrug-Resistant Enterococcus
faecalis in Hospital-Acquired Surgical Wound Infections and
Bacteremia: Concomitant Analysis of Antimicrobial Resistance
Genes |
title_short | Prevalence of Multidrug-Resistant Enterococcus
faecalis in Hospital-Acquired Surgical Wound Infections and
Bacteremia: Concomitant Analysis of Antimicrobial Resistance
Genes |
title_sort | prevalence of multidrug-resistant enterococcus
faecalis in hospital-acquired surgical wound infections and
bacteremia: concomitant analysis of antimicrobial resistance
genes |
topic | IDR-9 Antimicrobial Resistance |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6796195/ https://www.ncbi.nlm.nih.gov/pubmed/31662606 http://dx.doi.org/10.1177/1178633719882929 |
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