Dissociation of solid tumor tissues with cold active protease for single-cell RNA-seq minimizes conserved collagenase-associated stress responses

BACKGROUND: Single-cell RNA sequencing (scRNA-seq) is a powerful tool for studying complex biological systems, such as tumor heterogeneity and tissue microenvironments. However, the sources of technical and biological variation in primary solid tumor tissues and patient-derived mouse xenografts for...

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Detalles Bibliográficos
Autores principales: O’Flanagan, Ciara H., Campbell, Kieran R., Zhang, Allen W., Kabeer, Farhia, Lim, Jamie L. P., Biele, Justina, Eirew, Peter, Lai, Daniel, McPherson, Andrew, Kong, Esther, Bates, Cherie, Borkowski, Kelly, Wiens, Matt, Hewitson, Brittany, Hopkins, James, Pham, Jenifer, Ceglia, Nicholas, Moore, Richard, Mungall, Andrew J., McAlpine, Jessica N., Shah, Sohrab P., Aparicio, Samuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6796327/
https://www.ncbi.nlm.nih.gov/pubmed/31623682
http://dx.doi.org/10.1186/s13059-019-1830-0

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