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Characterization of Salmonella Dublin isolated from bovine and human hosts

BACKGROUND: Salmonella enterica subsp. enterica serovar Dublin (S. Dublin), a cattle adapted serovar causes enteritis, and systemic disease in bovines. The invasive index of this serovar far exceeds that of the other serovars and human infections often present as fatal or highly resistant infections...

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Autores principales: Paudyal, Narayan, Pan, Hang, Elbediwi, Mohammed, Zhou, Xiao, Peng, Xianqi, Li, Xiaoliang, Fang, Weihuan, Yue, Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6796477/
https://www.ncbi.nlm.nih.gov/pubmed/31619165
http://dx.doi.org/10.1186/s12866-019-1598-0
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author Paudyal, Narayan
Pan, Hang
Elbediwi, Mohammed
Zhou, Xiao
Peng, Xianqi
Li, Xiaoliang
Fang, Weihuan
Yue, Min
author_facet Paudyal, Narayan
Pan, Hang
Elbediwi, Mohammed
Zhou, Xiao
Peng, Xianqi
Li, Xiaoliang
Fang, Weihuan
Yue, Min
author_sort Paudyal, Narayan
collection PubMed
description BACKGROUND: Salmonella enterica subsp. enterica serovar Dublin (S. Dublin), a cattle adapted serovar causes enteritis, and systemic disease in bovines. The invasive index of this serovar far exceeds that of the other serovars and human infections often present as fatal or highly resistant infections. In this, observational study, phenotypic properties of human and bovine-derived isolates of S. Dublin along with antibiogram of common antimicrobials were evaluated. The multiplex PCR confirmed isolates were genotyped using 7-gene legacy MLST. MIC assay was done by broth microdilution method. Previously published protocols were used to assess the motility, biofilm formation and morphotype. Vi antigen was agglutinated using commercial antiserum. Caenorhabditis elegans infection model was used to evaluate the virulence potiential. Phenotyping experiments were done in duplicates while virulence assay was done in triplicates. Whole-genome sequencing was used to predict the genes responsible for acquired resistance and a genotype-phenotype comparison was made. RESULTS: We evaluated 96 bovine and 10 human isolates in this study. All the isolates belonged to ST10 in eBG53 and were negative for Vi-antigen. The swarming motility, biofilm formation and morphotype were variable in the isolates of both groups. Resistance to sulfamethoxazole, ampicillin, chloramphenicol, tetracycline was > 90% in animal isolates whereas resistance to sulfamethoxazole was > 70% in human isolates. MDR was also higher in animal isolates. Human isolates were significantly (P < 0.0001) more virulent than animal isolates on C. elegans infection model. The genomic comparison based on the core SNPs showed a high degree of homogeneity between the isolates. The carriage of IncA/C2 plasmid was seen as a typical feature of isolates from the bovine hosts. CONCLUSION: Human isolates showed more diversity in the phenotypic assays. Animal isolates showed a higher degree of antimicrobial resistance with greater MDR but human isolates formed more biofilm and had greater swarming motility as well as increased virulence to the nematode C. elegans. The carriage of IncA/C2 plasmid could contribute to the distinguishing feature of the bovine isolates. The tandem use of genotypic-phenotypic assays improves the understanding of diversity and differential behaviour of the same serovar from unrelated host sources.
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spelling pubmed-67964772019-10-21 Characterization of Salmonella Dublin isolated from bovine and human hosts Paudyal, Narayan Pan, Hang Elbediwi, Mohammed Zhou, Xiao Peng, Xianqi Li, Xiaoliang Fang, Weihuan Yue, Min BMC Microbiol Research Article BACKGROUND: Salmonella enterica subsp. enterica serovar Dublin (S. Dublin), a cattle adapted serovar causes enteritis, and systemic disease in bovines. The invasive index of this serovar far exceeds that of the other serovars and human infections often present as fatal or highly resistant infections. In this, observational study, phenotypic properties of human and bovine-derived isolates of S. Dublin along with antibiogram of common antimicrobials were evaluated. The multiplex PCR confirmed isolates were genotyped using 7-gene legacy MLST. MIC assay was done by broth microdilution method. Previously published protocols were used to assess the motility, biofilm formation and morphotype. Vi antigen was agglutinated using commercial antiserum. Caenorhabditis elegans infection model was used to evaluate the virulence potiential. Phenotyping experiments were done in duplicates while virulence assay was done in triplicates. Whole-genome sequencing was used to predict the genes responsible for acquired resistance and a genotype-phenotype comparison was made. RESULTS: We evaluated 96 bovine and 10 human isolates in this study. All the isolates belonged to ST10 in eBG53 and were negative for Vi-antigen. The swarming motility, biofilm formation and morphotype were variable in the isolates of both groups. Resistance to sulfamethoxazole, ampicillin, chloramphenicol, tetracycline was > 90% in animal isolates whereas resistance to sulfamethoxazole was > 70% in human isolates. MDR was also higher in animal isolates. Human isolates were significantly (P < 0.0001) more virulent than animal isolates on C. elegans infection model. The genomic comparison based on the core SNPs showed a high degree of homogeneity between the isolates. The carriage of IncA/C2 plasmid was seen as a typical feature of isolates from the bovine hosts. CONCLUSION: Human isolates showed more diversity in the phenotypic assays. Animal isolates showed a higher degree of antimicrobial resistance with greater MDR but human isolates formed more biofilm and had greater swarming motility as well as increased virulence to the nematode C. elegans. The carriage of IncA/C2 plasmid could contribute to the distinguishing feature of the bovine isolates. The tandem use of genotypic-phenotypic assays improves the understanding of diversity and differential behaviour of the same serovar from unrelated host sources. BioMed Central 2019-10-16 /pmc/articles/PMC6796477/ /pubmed/31619165 http://dx.doi.org/10.1186/s12866-019-1598-0 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Paudyal, Narayan
Pan, Hang
Elbediwi, Mohammed
Zhou, Xiao
Peng, Xianqi
Li, Xiaoliang
Fang, Weihuan
Yue, Min
Characterization of Salmonella Dublin isolated from bovine and human hosts
title Characterization of Salmonella Dublin isolated from bovine and human hosts
title_full Characterization of Salmonella Dublin isolated from bovine and human hosts
title_fullStr Characterization of Salmonella Dublin isolated from bovine and human hosts
title_full_unstemmed Characterization of Salmonella Dublin isolated from bovine and human hosts
title_short Characterization of Salmonella Dublin isolated from bovine and human hosts
title_sort characterization of salmonella dublin isolated from bovine and human hosts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6796477/
https://www.ncbi.nlm.nih.gov/pubmed/31619165
http://dx.doi.org/10.1186/s12866-019-1598-0
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