Cargando…

Cas9 has no exonuclease activity resulting in staggered cleavage with overhangs and predictable di- and tri-nucleotide CRISPR insertions without template donor

Detalles Bibliográficos
Autores principales: Shi, Xin, Shou, Jia, Mehryar, Mohammadreza M., Li, Jingwei, Wang, Leyang, Zhang, Mo, Huang, Haiyan, Sun, Xiaofang, Wu, Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6796948/
https://www.ncbi.nlm.nih.gov/pubmed/31636963
http://dx.doi.org/10.1038/s41421-019-0120-z
_version_ 1783459718726942720
author Shi, Xin
Shou, Jia
Mehryar, Mohammadreza M.
Li, Jingwei
Wang, Leyang
Zhang, Mo
Huang, Haiyan
Sun, Xiaofang
Wu, Qiang
author_facet Shi, Xin
Shou, Jia
Mehryar, Mohammadreza M.
Li, Jingwei
Wang, Leyang
Zhang, Mo
Huang, Haiyan
Sun, Xiaofang
Wu, Qiang
author_sort Shi, Xin
collection PubMed
description
format Online
Article
Text
id pubmed-6796948
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-67969482019-10-21 Cas9 has no exonuclease activity resulting in staggered cleavage with overhangs and predictable di- and tri-nucleotide CRISPR insertions without template donor Shi, Xin Shou, Jia Mehryar, Mohammadreza M. Li, Jingwei Wang, Leyang Zhang, Mo Huang, Haiyan Sun, Xiaofang Wu, Qiang Cell Discov Correspondence Nature Publishing Group UK 2019-10-01 /pmc/articles/PMC6796948/ /pubmed/31636963 http://dx.doi.org/10.1038/s41421-019-0120-z Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Correspondence
Shi, Xin
Shou, Jia
Mehryar, Mohammadreza M.
Li, Jingwei
Wang, Leyang
Zhang, Mo
Huang, Haiyan
Sun, Xiaofang
Wu, Qiang
Cas9 has no exonuclease activity resulting in staggered cleavage with overhangs and predictable di- and tri-nucleotide CRISPR insertions without template donor
title Cas9 has no exonuclease activity resulting in staggered cleavage with overhangs and predictable di- and tri-nucleotide CRISPR insertions without template donor
title_full Cas9 has no exonuclease activity resulting in staggered cleavage with overhangs and predictable di- and tri-nucleotide CRISPR insertions without template donor
title_fullStr Cas9 has no exonuclease activity resulting in staggered cleavage with overhangs and predictable di- and tri-nucleotide CRISPR insertions without template donor
title_full_unstemmed Cas9 has no exonuclease activity resulting in staggered cleavage with overhangs and predictable di- and tri-nucleotide CRISPR insertions without template donor
title_short Cas9 has no exonuclease activity resulting in staggered cleavage with overhangs and predictable di- and tri-nucleotide CRISPR insertions without template donor
title_sort cas9 has no exonuclease activity resulting in staggered cleavage with overhangs and predictable di- and tri-nucleotide crispr insertions without template donor
topic Correspondence
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6796948/
https://www.ncbi.nlm.nih.gov/pubmed/31636963
http://dx.doi.org/10.1038/s41421-019-0120-z
work_keys_str_mv AT shixin cas9hasnoexonucleaseactivityresultinginstaggeredcleavagewithoverhangsandpredictablediandtrinucleotidecrisprinsertionswithouttemplatedonor
AT shoujia cas9hasnoexonucleaseactivityresultinginstaggeredcleavagewithoverhangsandpredictablediandtrinucleotidecrisprinsertionswithouttemplatedonor
AT mehryarmohammadrezam cas9hasnoexonucleaseactivityresultinginstaggeredcleavagewithoverhangsandpredictablediandtrinucleotidecrisprinsertionswithouttemplatedonor
AT lijingwei cas9hasnoexonucleaseactivityresultinginstaggeredcleavagewithoverhangsandpredictablediandtrinucleotidecrisprinsertionswithouttemplatedonor
AT wangleyang cas9hasnoexonucleaseactivityresultinginstaggeredcleavagewithoverhangsandpredictablediandtrinucleotidecrisprinsertionswithouttemplatedonor
AT zhangmo cas9hasnoexonucleaseactivityresultinginstaggeredcleavagewithoverhangsandpredictablediandtrinucleotidecrisprinsertionswithouttemplatedonor
AT huanghaiyan cas9hasnoexonucleaseactivityresultinginstaggeredcleavagewithoverhangsandpredictablediandtrinucleotidecrisprinsertionswithouttemplatedonor
AT sunxiaofang cas9hasnoexonucleaseactivityresultinginstaggeredcleavagewithoverhangsandpredictablediandtrinucleotidecrisprinsertionswithouttemplatedonor
AT wuqiang cas9hasnoexonucleaseactivityresultinginstaggeredcleavagewithoverhangsandpredictablediandtrinucleotidecrisprinsertionswithouttemplatedonor