Cargando…
Membrane-Protected Molecularly Imprinted Polymer for the Microextraction of Indole-3-butyric Acid in Mung Bean Sprouts
[Image: see text] Based on the hollow fiber protected molecularly imprinted polymer, a micro-solid-phase extraction (μ-SPE) method was developed and applied for the analysis of indole-3-butyric acid in mung bean sprouts by high-performance liquid chromatography. The extraction conditions of the μ-SP...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2019
|
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6796984/ https://www.ncbi.nlm.nih.gov/pubmed/31646224 http://dx.doi.org/10.1021/acsomega.9b01550 |
_version_ | 1783459727499329536 |
---|---|
author | Aihebaier, Sailemayi Muhammad, Turghun Wei, Aixia Mamat, Anwar Abuduaini, Munira Pataer, Parezhati Yigaimu, Aziguli Yimit, Abliz |
author_facet | Aihebaier, Sailemayi Muhammad, Turghun Wei, Aixia Mamat, Anwar Abuduaini, Munira Pataer, Parezhati Yigaimu, Aziguli Yimit, Abliz |
author_sort | Aihebaier, Sailemayi |
collection | PubMed |
description | [Image: see text] Based on the hollow fiber protected molecularly imprinted polymer, a micro-solid-phase extraction (μ-SPE) method was developed and applied for the analysis of indole-3-butyric acid in mung bean sprouts by high-performance liquid chromatography. The extraction conditions of the μ-SPE method were optimized using L(9)(3(4)) orthogonal, and optimum conditions were found as follows: pH of sample solution was 2.0, chloroform was the organic solvent for embedding the μ-SPE bars, and acetonitrile was the desorption solvent. In addition, the extraction time was 80 min, desorption time was 5 min, stirring speed was 800 rpm, and concentration of NaCl was 10%. Under the optimum conditions, a standard curve was established for IBA, with a correlation coefficient of 0.9999. After extraction with phosphate buffer solution (pH = 9.0), successful pretreatment of mung bean sprouts was achieved by the μ-SPE method. The limit of detection was 0.075 mg/kg, and the recoveries were found to be in the range of 88.9–106.4%. This method is simple, environmentally friendly, and can be used for the determination of indole auxin contents in green bean sprouts quickly and accurately. |
format | Online Article Text |
id | pubmed-6796984 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-67969842019-10-23 Membrane-Protected Molecularly Imprinted Polymer for the Microextraction of Indole-3-butyric Acid in Mung Bean Sprouts Aihebaier, Sailemayi Muhammad, Turghun Wei, Aixia Mamat, Anwar Abuduaini, Munira Pataer, Parezhati Yigaimu, Aziguli Yimit, Abliz ACS Omega [Image: see text] Based on the hollow fiber protected molecularly imprinted polymer, a micro-solid-phase extraction (μ-SPE) method was developed and applied for the analysis of indole-3-butyric acid in mung bean sprouts by high-performance liquid chromatography. The extraction conditions of the μ-SPE method were optimized using L(9)(3(4)) orthogonal, and optimum conditions were found as follows: pH of sample solution was 2.0, chloroform was the organic solvent for embedding the μ-SPE bars, and acetonitrile was the desorption solvent. In addition, the extraction time was 80 min, desorption time was 5 min, stirring speed was 800 rpm, and concentration of NaCl was 10%. Under the optimum conditions, a standard curve was established for IBA, with a correlation coefficient of 0.9999. After extraction with phosphate buffer solution (pH = 9.0), successful pretreatment of mung bean sprouts was achieved by the μ-SPE method. The limit of detection was 0.075 mg/kg, and the recoveries were found to be in the range of 88.9–106.4%. This method is simple, environmentally friendly, and can be used for the determination of indole auxin contents in green bean sprouts quickly and accurately. American Chemical Society 2019-10-01 /pmc/articles/PMC6796984/ /pubmed/31646224 http://dx.doi.org/10.1021/acsomega.9b01550 Text en Copyright © 2019 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. |
spellingShingle | Aihebaier, Sailemayi Muhammad, Turghun Wei, Aixia Mamat, Anwar Abuduaini, Munira Pataer, Parezhati Yigaimu, Aziguli Yimit, Abliz Membrane-Protected Molecularly Imprinted Polymer for the Microextraction of Indole-3-butyric Acid in Mung Bean Sprouts |
title | Membrane-Protected Molecularly Imprinted Polymer for
the Microextraction of Indole-3-butyric Acid in Mung Bean Sprouts |
title_full | Membrane-Protected Molecularly Imprinted Polymer for
the Microextraction of Indole-3-butyric Acid in Mung Bean Sprouts |
title_fullStr | Membrane-Protected Molecularly Imprinted Polymer for
the Microextraction of Indole-3-butyric Acid in Mung Bean Sprouts |
title_full_unstemmed | Membrane-Protected Molecularly Imprinted Polymer for
the Microextraction of Indole-3-butyric Acid in Mung Bean Sprouts |
title_short | Membrane-Protected Molecularly Imprinted Polymer for
the Microextraction of Indole-3-butyric Acid in Mung Bean Sprouts |
title_sort | membrane-protected molecularly imprinted polymer for
the microextraction of indole-3-butyric acid in mung bean sprouts |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6796984/ https://www.ncbi.nlm.nih.gov/pubmed/31646224 http://dx.doi.org/10.1021/acsomega.9b01550 |
work_keys_str_mv | AT aihebaiersailemayi membraneprotectedmolecularlyimprintedpolymerforthemicroextractionofindole3butyricacidinmungbeansprouts AT muhammadturghun membraneprotectedmolecularlyimprintedpolymerforthemicroextractionofindole3butyricacidinmungbeansprouts AT weiaixia membraneprotectedmolecularlyimprintedpolymerforthemicroextractionofindole3butyricacidinmungbeansprouts AT mamatanwar membraneprotectedmolecularlyimprintedpolymerforthemicroextractionofindole3butyricacidinmungbeansprouts AT abuduainimunira membraneprotectedmolecularlyimprintedpolymerforthemicroextractionofindole3butyricacidinmungbeansprouts AT pataerparezhati membraneprotectedmolecularlyimprintedpolymerforthemicroextractionofindole3butyricacidinmungbeansprouts AT yigaimuaziguli membraneprotectedmolecularlyimprintedpolymerforthemicroextractionofindole3butyricacidinmungbeansprouts AT yimitabliz membraneprotectedmolecularlyimprintedpolymerforthemicroextractionofindole3butyricacidinmungbeansprouts |