Cargando…

Identification of protein changes in the blood plasma of lung cancer patients subjected to chemotherapy using a 2D-DIGE approach

A comparative analysis of blood samples (depleted of albumin and IgG) obtained from lung cancer patients before chemotherapy versus after a second cycle of chemotherapy was performed using two-dimensional difference gel electrophoresis (2D-DIGE). The control group consisted of eight patients with no...

Descripción completa

Detalles Bibliográficos
Autores principales: Ciereszko, Andrzej, Dietrich, Mariola A., Słowińska, Mariola, Nynca, Joanna, Ciborowski, Michał, Kisluk, Joanna, Michalska-Falkowska, Anna, Reszec, Joanna, Sierko, Ewa, Nikliński, Jacek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6797170/
https://www.ncbi.nlm.nih.gov/pubmed/31622403
http://dx.doi.org/10.1371/journal.pone.0223840
_version_ 1783459759832170496
author Ciereszko, Andrzej
Dietrich, Mariola A.
Słowińska, Mariola
Nynca, Joanna
Ciborowski, Michał
Kisluk, Joanna
Michalska-Falkowska, Anna
Reszec, Joanna
Sierko, Ewa
Nikliński, Jacek
author_facet Ciereszko, Andrzej
Dietrich, Mariola A.
Słowińska, Mariola
Nynca, Joanna
Ciborowski, Michał
Kisluk, Joanna
Michalska-Falkowska, Anna
Reszec, Joanna
Sierko, Ewa
Nikliński, Jacek
author_sort Ciereszko, Andrzej
collection PubMed
description A comparative analysis of blood samples (depleted of albumin and IgG) obtained from lung cancer patients before chemotherapy versus after a second cycle of chemotherapy was performed using two-dimensional difference gel electrophoresis (2D-DIGE). The control group consisted of eight patients with non-cancerous lung diseases, and the experimental group consisted of four adenocarcinoma (ADC) and four squamous cell carcinoma (SCC) patients. Analyses of gels revealed significant changes in proteins and/or their proteoforms between control patients and lung cancer patients, both before and after a second cycle of chemotherapy. Most of these proteins were related to inflammation, including acute phase proteins (APPs) such as forms of haptoglobin and transferrin, complement component C3, and clusterin. The variable expression of APPs can potentially be used for profiling lung cancer. The greatest changes observed after chemotherapy were in transferrin and serotransferrin, which likely reflect disturbances in iron turnover after chemotherapy-induced anaemia. Significant changes in plasma proteins between ADC and SCC patients were also revealed, suggesting use of plasma vitronectin as a potential marker of SCC.
format Online
Article
Text
id pubmed-6797170
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-67971702019-10-25 Identification of protein changes in the blood plasma of lung cancer patients subjected to chemotherapy using a 2D-DIGE approach Ciereszko, Andrzej Dietrich, Mariola A. Słowińska, Mariola Nynca, Joanna Ciborowski, Michał Kisluk, Joanna Michalska-Falkowska, Anna Reszec, Joanna Sierko, Ewa Nikliński, Jacek PLoS One Research Article A comparative analysis of blood samples (depleted of albumin and IgG) obtained from lung cancer patients before chemotherapy versus after a second cycle of chemotherapy was performed using two-dimensional difference gel electrophoresis (2D-DIGE). The control group consisted of eight patients with non-cancerous lung diseases, and the experimental group consisted of four adenocarcinoma (ADC) and four squamous cell carcinoma (SCC) patients. Analyses of gels revealed significant changes in proteins and/or their proteoforms between control patients and lung cancer patients, both before and after a second cycle of chemotherapy. Most of these proteins were related to inflammation, including acute phase proteins (APPs) such as forms of haptoglobin and transferrin, complement component C3, and clusterin. The variable expression of APPs can potentially be used for profiling lung cancer. The greatest changes observed after chemotherapy were in transferrin and serotransferrin, which likely reflect disturbances in iron turnover after chemotherapy-induced anaemia. Significant changes in plasma proteins between ADC and SCC patients were also revealed, suggesting use of plasma vitronectin as a potential marker of SCC. Public Library of Science 2019-10-17 /pmc/articles/PMC6797170/ /pubmed/31622403 http://dx.doi.org/10.1371/journal.pone.0223840 Text en © 2019 Ciereszko et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ciereszko, Andrzej
Dietrich, Mariola A.
Słowińska, Mariola
Nynca, Joanna
Ciborowski, Michał
Kisluk, Joanna
Michalska-Falkowska, Anna
Reszec, Joanna
Sierko, Ewa
Nikliński, Jacek
Identification of protein changes in the blood plasma of lung cancer patients subjected to chemotherapy using a 2D-DIGE approach
title Identification of protein changes in the blood plasma of lung cancer patients subjected to chemotherapy using a 2D-DIGE approach
title_full Identification of protein changes in the blood plasma of lung cancer patients subjected to chemotherapy using a 2D-DIGE approach
title_fullStr Identification of protein changes in the blood plasma of lung cancer patients subjected to chemotherapy using a 2D-DIGE approach
title_full_unstemmed Identification of protein changes in the blood plasma of lung cancer patients subjected to chemotherapy using a 2D-DIGE approach
title_short Identification of protein changes in the blood plasma of lung cancer patients subjected to chemotherapy using a 2D-DIGE approach
title_sort identification of protein changes in the blood plasma of lung cancer patients subjected to chemotherapy using a 2d-dige approach
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6797170/
https://www.ncbi.nlm.nih.gov/pubmed/31622403
http://dx.doi.org/10.1371/journal.pone.0223840
work_keys_str_mv AT ciereszkoandrzej identificationofproteinchangesinthebloodplasmaoflungcancerpatientssubjectedtochemotherapyusinga2ddigeapproach
AT dietrichmariolaa identificationofproteinchangesinthebloodplasmaoflungcancerpatientssubjectedtochemotherapyusinga2ddigeapproach
AT słowinskamariola identificationofproteinchangesinthebloodplasmaoflungcancerpatientssubjectedtochemotherapyusinga2ddigeapproach
AT nyncajoanna identificationofproteinchangesinthebloodplasmaoflungcancerpatientssubjectedtochemotherapyusinga2ddigeapproach
AT ciborowskimichał identificationofproteinchangesinthebloodplasmaoflungcancerpatientssubjectedtochemotherapyusinga2ddigeapproach
AT kislukjoanna identificationofproteinchangesinthebloodplasmaoflungcancerpatientssubjectedtochemotherapyusinga2ddigeapproach
AT michalskafalkowskaanna identificationofproteinchangesinthebloodplasmaoflungcancerpatientssubjectedtochemotherapyusinga2ddigeapproach
AT reszecjoanna identificationofproteinchangesinthebloodplasmaoflungcancerpatientssubjectedtochemotherapyusinga2ddigeapproach
AT sierkoewa identificationofproteinchangesinthebloodplasmaoflungcancerpatientssubjectedtochemotherapyusinga2ddigeapproach
AT niklinskijacek identificationofproteinchangesinthebloodplasmaoflungcancerpatientssubjectedtochemotherapyusinga2ddigeapproach