Establishing Renal Proximal Tubule Epithelial-Derived Cell Lines Expressing Human Telomerase Reverse Transcriptase for Studying BK Polyomavirus

We previously established an infection model for BK polyomavirus (BKPyV) in primary human renal proximal tubule epithelial (RPTE) cells. Use of these cells is limited by their inability to be passaged extensively. Here, we describe RPTE cells immortalized with human telomerase reverse transcriptase...

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Detalles Bibliográficos
Autores principales: Zhao, Linbo, Imperiale, Michael J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2019
Materias:
Culture Collections/Mutant Libraries
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6797542/
https://www.ncbi.nlm.nih.gov/pubmed/31624153
http://dx.doi.org/10.1128/MRA.01129-19
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author Zhao, Linbo
Imperiale, Michael J.
author_facet Zhao, Linbo
Imperiale, Michael J.
author_sort Zhao, Linbo
collection PubMed
description We previously established an infection model for BK polyomavirus (BKPyV) in primary human renal proximal tubule epithelial (RPTE) cells. Use of these cells is limited by their inability to be passaged extensively. Here, we describe RPTE cells immortalized with human telomerase reverse transcriptase (hTERT), which can serve as a model system for acute or persistent BKPyV infection.
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spelling pubmed-67975422019-10-21 Establishing Renal Proximal Tubule Epithelial-Derived Cell Lines Expressing Human Telomerase Reverse Transcriptase for Studying BK Polyomavirus Zhao, Linbo Imperiale, Michael J. Microbiol Resour Announc Culture Collections/Mutant Libraries We previously established an infection model for BK polyomavirus (BKPyV) in primary human renal proximal tubule epithelial (RPTE) cells. Use of these cells is limited by their inability to be passaged extensively. Here, we describe RPTE cells immortalized with human telomerase reverse transcriptase (hTERT), which can serve as a model system for acute or persistent BKPyV infection. American Society for Microbiology 2019-10-17 /pmc/articles/PMC6797542/ /pubmed/31624153 http://dx.doi.org/10.1128/MRA.01129-19 Text en Copyright © 2019 Zhao and Imperiale. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Culture Collections/Mutant Libraries
Zhao, Linbo
Imperiale, Michael J.
Establishing Renal Proximal Tubule Epithelial-Derived Cell Lines Expressing Human Telomerase Reverse Transcriptase for Studying BK Polyomavirus
title Establishing Renal Proximal Tubule Epithelial-Derived Cell Lines Expressing Human Telomerase Reverse Transcriptase for Studying BK Polyomavirus
title_full Establishing Renal Proximal Tubule Epithelial-Derived Cell Lines Expressing Human Telomerase Reverse Transcriptase for Studying BK Polyomavirus
title_fullStr Establishing Renal Proximal Tubule Epithelial-Derived Cell Lines Expressing Human Telomerase Reverse Transcriptase for Studying BK Polyomavirus
title_full_unstemmed Establishing Renal Proximal Tubule Epithelial-Derived Cell Lines Expressing Human Telomerase Reverse Transcriptase for Studying BK Polyomavirus
title_short Establishing Renal Proximal Tubule Epithelial-Derived Cell Lines Expressing Human Telomerase Reverse Transcriptase for Studying BK Polyomavirus
title_sort establishing renal proximal tubule epithelial-derived cell lines expressing human telomerase reverse transcriptase for studying bk polyomavirus
topic Culture Collections/Mutant Libraries
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6797542/
https://www.ncbi.nlm.nih.gov/pubmed/31624153
http://dx.doi.org/10.1128/MRA.01129-19
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AT imperialemichaelj establishingrenalproximaltubuleepithelialderivedcelllinesexpressinghumantelomerasereversetranscriptaseforstudyingbkpolyomavirus

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