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Colonizing multidrug-resistant bacteria and the longitudinal evolution of the intestinal microbiome after liver transplantation
Infections by multidrug-resistant bacteria (MDRB) remain a leading cause of morbidity and mortality after liver transplantation (LT). Gut dysbiosis characteristic of end-stage liver disease may predispose patients to intestinal MDRB colonization and infection, in turn exacerbating dysbiosis. However...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6797753/ https://www.ncbi.nlm.nih.gov/pubmed/31624266 http://dx.doi.org/10.1038/s41467-019-12633-4 |
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author | Annavajhala, Medini K. Gomez-Simmonds, Angela Macesic, Nenad Sullivan, Sean B. Kress, Anna Khan, Sabrina D. Giddins, Marla J. Stump, Stephania Kim, Grace I. Narain, Ryan Verna, Elizabeth C. Uhlemann, Anne-Catrin |
author_facet | Annavajhala, Medini K. Gomez-Simmonds, Angela Macesic, Nenad Sullivan, Sean B. Kress, Anna Khan, Sabrina D. Giddins, Marla J. Stump, Stephania Kim, Grace I. Narain, Ryan Verna, Elizabeth C. Uhlemann, Anne-Catrin |
author_sort | Annavajhala, Medini K. |
collection | PubMed |
description | Infections by multidrug-resistant bacteria (MDRB) remain a leading cause of morbidity and mortality after liver transplantation (LT). Gut dysbiosis characteristic of end-stage liver disease may predispose patients to intestinal MDRB colonization and infection, in turn exacerbating dysbiosis. However, relationships between MDRB colonization and dysbiosis after LT remain unclear. We prospectively recruited 177 adult patients undergoing LT at a single tertiary care center. 16 S V3-V4 rRNA sequencing was performed on 723 fecal samples collected pre-LT and periodically until one-year post-LT to test whether MDRB colonization was associated with decreased microbiome diversity. In multivariate linear mixed-effect models, MDRB colonization predicts reduced Shannon α-diversity, after controlling for underlying liver disease, antibiotic exposures, and clinical complications. Importantly, pre-LT microbial markers predict subsequent colonization by MDRB. Our results suggest MDRB colonization as a major, previously unrecognized, marker of persistent dysbiosis. Therapeutic approaches accounting for microbial and clinical factors are needed to address post-transplant microbiome health. |
format | Online Article Text |
id | pubmed-6797753 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-67977532019-10-21 Colonizing multidrug-resistant bacteria and the longitudinal evolution of the intestinal microbiome after liver transplantation Annavajhala, Medini K. Gomez-Simmonds, Angela Macesic, Nenad Sullivan, Sean B. Kress, Anna Khan, Sabrina D. Giddins, Marla J. Stump, Stephania Kim, Grace I. Narain, Ryan Verna, Elizabeth C. Uhlemann, Anne-Catrin Nat Commun Article Infections by multidrug-resistant bacteria (MDRB) remain a leading cause of morbidity and mortality after liver transplantation (LT). Gut dysbiosis characteristic of end-stage liver disease may predispose patients to intestinal MDRB colonization and infection, in turn exacerbating dysbiosis. However, relationships between MDRB colonization and dysbiosis after LT remain unclear. We prospectively recruited 177 adult patients undergoing LT at a single tertiary care center. 16 S V3-V4 rRNA sequencing was performed on 723 fecal samples collected pre-LT and periodically until one-year post-LT to test whether MDRB colonization was associated with decreased microbiome diversity. In multivariate linear mixed-effect models, MDRB colonization predicts reduced Shannon α-diversity, after controlling for underlying liver disease, antibiotic exposures, and clinical complications. Importantly, pre-LT microbial markers predict subsequent colonization by MDRB. Our results suggest MDRB colonization as a major, previously unrecognized, marker of persistent dysbiosis. Therapeutic approaches accounting for microbial and clinical factors are needed to address post-transplant microbiome health. Nature Publishing Group UK 2019-10-17 /pmc/articles/PMC6797753/ /pubmed/31624266 http://dx.doi.org/10.1038/s41467-019-12633-4 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Annavajhala, Medini K. Gomez-Simmonds, Angela Macesic, Nenad Sullivan, Sean B. Kress, Anna Khan, Sabrina D. Giddins, Marla J. Stump, Stephania Kim, Grace I. Narain, Ryan Verna, Elizabeth C. Uhlemann, Anne-Catrin Colonizing multidrug-resistant bacteria and the longitudinal evolution of the intestinal microbiome after liver transplantation |
title | Colonizing multidrug-resistant bacteria and the longitudinal evolution of the intestinal microbiome after liver transplantation |
title_full | Colonizing multidrug-resistant bacteria and the longitudinal evolution of the intestinal microbiome after liver transplantation |
title_fullStr | Colonizing multidrug-resistant bacteria and the longitudinal evolution of the intestinal microbiome after liver transplantation |
title_full_unstemmed | Colonizing multidrug-resistant bacteria and the longitudinal evolution of the intestinal microbiome after liver transplantation |
title_short | Colonizing multidrug-resistant bacteria and the longitudinal evolution of the intestinal microbiome after liver transplantation |
title_sort | colonizing multidrug-resistant bacteria and the longitudinal evolution of the intestinal microbiome after liver transplantation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6797753/ https://www.ncbi.nlm.nih.gov/pubmed/31624266 http://dx.doi.org/10.1038/s41467-019-12633-4 |
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