The Codelivery of siRNA and QDs by pH-Responsive Micelle for Hepatoma Cancer Cells

Recently, RNA interfering (RNAi) has become a promising approach for cancer therapy. However, the application of RNAi for clinics is still hindered due to the lack of safe and efficient carriers. In this study, a pH-responsive micelle based on polycaprolactone-block–poly 2-(dimethylamino)ethyl metha...

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Autores principales: Cao, Zhonglin, Xiao, Huiyu, Li, Li, Liu, Maixian, Lin, Guimiao, Zhai, Peng, Yong, Ken-Tye, Wang, Xiaomei, Xu, Gaixia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Pharmacology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6797843/
https://www.ncbi.nlm.nih.gov/pubmed/31680969
http://dx.doi.org/10.3389/fphar.2019.01194
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author Cao, Zhonglin
Xiao, Huiyu
Li, Li
Liu, Maixian
Lin, Guimiao
Zhai, Peng
Yong, Ken-Tye
Wang, Xiaomei
Xu, Gaixia
author_facet Cao, Zhonglin
Xiao, Huiyu
Li, Li
Liu, Maixian
Lin, Guimiao
Zhai, Peng
Yong, Ken-Tye
Wang, Xiaomei
Xu, Gaixia
author_sort Cao, Zhonglin
collection PubMed
description Recently, RNA interfering (RNAi) has become a promising approach for cancer therapy. However, the application of RNAi for clinics is still hindered due to the lack of safe and efficient carriers. In this study, a pH-responsive micelle based on polycaprolactone-block–poly 2-(dimethylamino)ethyl methacrylate (PCL-PDEM) cationic copolymer was developed to carry short interfering RNA (siRNA) for silencing interleukin 8 (IL-8) gene in hepatoma cancer cells. The transfection efficiency of the PCL-PDEM-siRNA/quantum dots (QDs) nanoplex has reached about 70%, and the expression level of IL-8 decreased about 63%. Furthermore, the codelivery of QDs and siRNA has been realized, which is beneficial to visualize the process of siRNA delivery. No considerable cytotoxicity from the nanoparticles has been observed, indicating that our responsive cationic micelle is potential in clinical trial for hepatoma cancer therapy.
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spelling pubmed-67978432019-11-01 The Codelivery of siRNA and QDs by pH-Responsive Micelle for Hepatoma Cancer Cells Cao, Zhonglin Xiao, Huiyu Li, Li Liu, Maixian Lin, Guimiao Zhai, Peng Yong, Ken-Tye Wang, Xiaomei Xu, Gaixia Front Pharmacol Pharmacology Recently, RNA interfering (RNAi) has become a promising approach for cancer therapy. However, the application of RNAi for clinics is still hindered due to the lack of safe and efficient carriers. In this study, a pH-responsive micelle based on polycaprolactone-block–poly 2-(dimethylamino)ethyl methacrylate (PCL-PDEM) cationic copolymer was developed to carry short interfering RNA (siRNA) for silencing interleukin 8 (IL-8) gene in hepatoma cancer cells. The transfection efficiency of the PCL-PDEM-siRNA/quantum dots (QDs) nanoplex has reached about 70%, and the expression level of IL-8 decreased about 63%. Furthermore, the codelivery of QDs and siRNA has been realized, which is beneficial to visualize the process of siRNA delivery. No considerable cytotoxicity from the nanoparticles has been observed, indicating that our responsive cationic micelle is potential in clinical trial for hepatoma cancer therapy. Frontiers Media S.A. 2019-10-10 /pmc/articles/PMC6797843/ /pubmed/31680969 http://dx.doi.org/10.3389/fphar.2019.01194 Text en Copyright © 2019 Cao, Xiao, Li, Liu, Lin, Zhai, Yong, Wang and Xu http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Cao, Zhonglin
Xiao, Huiyu
Li, Li
Liu, Maixian
Lin, Guimiao
Zhai, Peng
Yong, Ken-Tye
Wang, Xiaomei
Xu, Gaixia
The Codelivery of siRNA and QDs by pH-Responsive Micelle for Hepatoma Cancer Cells
title The Codelivery of siRNA and QDs by pH-Responsive Micelle for Hepatoma Cancer Cells
title_full The Codelivery of siRNA and QDs by pH-Responsive Micelle for Hepatoma Cancer Cells
title_fullStr The Codelivery of siRNA and QDs by pH-Responsive Micelle for Hepatoma Cancer Cells
title_full_unstemmed The Codelivery of siRNA and QDs by pH-Responsive Micelle for Hepatoma Cancer Cells
title_short The Codelivery of siRNA and QDs by pH-Responsive Micelle for Hepatoma Cancer Cells
title_sort codelivery of sirna and qds by ph-responsive micelle for hepatoma cancer cells
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6797843/
https://www.ncbi.nlm.nih.gov/pubmed/31680969
http://dx.doi.org/10.3389/fphar.2019.01194
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