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Delay in hepatocyte proliferation and prostaglandin D2 synthase expression for cholestasis due to endotoxin during partial hepatectomy in rats
Infection is a frequent complication of liver transplantation or partial hepatectomy (PH) and sometimes results in cholestasis. We examined factors involved in infection-induced cholestasis after PH, employing a rat PH model and lipopolysaccharide (LPS) as a bacterial toxin. Male Sprague-Dawley rats...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6797974/ https://www.ncbi.nlm.nih.gov/pubmed/31545425 http://dx.doi.org/10.3892/mmr.2019.10681 |
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author | Wakasa, Yusuke Kimura, Norihisa Yamada, Toshiyuki Shimizu, Takeshi Hakamada, Kenichi Tsuchida, Shigeki |
author_facet | Wakasa, Yusuke Kimura, Norihisa Yamada, Toshiyuki Shimizu, Takeshi Hakamada, Kenichi Tsuchida, Shigeki |
author_sort | Wakasa, Yusuke |
collection | PubMed |
description | Infection is a frequent complication of liver transplantation or partial hepatectomy (PH) and sometimes results in cholestasis. We examined factors involved in infection-induced cholestasis after PH, employing a rat PH model and lipopolysaccharide (LPS) as a bacterial toxin. Male Sprague-Dawley rats were subjected to 70% PH and/or LPS injection, and tissues were harvested at 0, 24, 72 and 168 h. Gene expression was analyzed by microarray analysis and reverse transcription-quantitative polymerase chain reaction, and protein levels and localization were analyzed by western blotting and immunohistochemistry, respectively. Plasma bile acid levels were significantly higher in the LPS + PH group than in the PH group. Ribonucleotide reductase regulatory subunit M2 and proliferating cell nuclear antigen peaked at 24 and 72 h in the PH group and LPS + PH group, respectively, indicating a delay in cell proliferation in the latter group. The sodium-dependent taurocholate cotransporting polypeptide and organic-anion-transporting polypeptide 1a1 and 1a2 were reduced in the PH group at 24 h, and were not further decreased in the LPS + PH group. Chemokine ligand 9 (Cxcl9), a chemokine involved in M2 macrophage polarization, increased after 24 h in the LPS and the LPS + PH groups. The number and shape of Cxcl9-positive cells were similar to CD163-positive cells, suggesting that such cells produced the chemokine. Hematopoietic prostaglandin D(2) synthase (Ptgds2) was only detected in hepatocytes of the LPS + PH group exhibiting a delay in cell proliferation. Thus, Kupffer cells activated with LPS were suggested to be responsible for a delay in hepatocyte proliferation after PH. |
format | Online Article Text |
id | pubmed-6797974 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-67979742019-10-22 Delay in hepatocyte proliferation and prostaglandin D2 synthase expression for cholestasis due to endotoxin during partial hepatectomy in rats Wakasa, Yusuke Kimura, Norihisa Yamada, Toshiyuki Shimizu, Takeshi Hakamada, Kenichi Tsuchida, Shigeki Mol Med Rep Articles Infection is a frequent complication of liver transplantation or partial hepatectomy (PH) and sometimes results in cholestasis. We examined factors involved in infection-induced cholestasis after PH, employing a rat PH model and lipopolysaccharide (LPS) as a bacterial toxin. Male Sprague-Dawley rats were subjected to 70% PH and/or LPS injection, and tissues were harvested at 0, 24, 72 and 168 h. Gene expression was analyzed by microarray analysis and reverse transcription-quantitative polymerase chain reaction, and protein levels and localization were analyzed by western blotting and immunohistochemistry, respectively. Plasma bile acid levels were significantly higher in the LPS + PH group than in the PH group. Ribonucleotide reductase regulatory subunit M2 and proliferating cell nuclear antigen peaked at 24 and 72 h in the PH group and LPS + PH group, respectively, indicating a delay in cell proliferation in the latter group. The sodium-dependent taurocholate cotransporting polypeptide and organic-anion-transporting polypeptide 1a1 and 1a2 were reduced in the PH group at 24 h, and were not further decreased in the LPS + PH group. Chemokine ligand 9 (Cxcl9), a chemokine involved in M2 macrophage polarization, increased after 24 h in the LPS and the LPS + PH groups. The number and shape of Cxcl9-positive cells were similar to CD163-positive cells, suggesting that such cells produced the chemokine. Hematopoietic prostaglandin D(2) synthase (Ptgds2) was only detected in hepatocytes of the LPS + PH group exhibiting a delay in cell proliferation. Thus, Kupffer cells activated with LPS were suggested to be responsible for a delay in hepatocyte proliferation after PH. D.A. Spandidos 2019-11 2019-09-13 /pmc/articles/PMC6797974/ /pubmed/31545425 http://dx.doi.org/10.3892/mmr.2019.10681 Text en Copyright: © Wakasa et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Wakasa, Yusuke Kimura, Norihisa Yamada, Toshiyuki Shimizu, Takeshi Hakamada, Kenichi Tsuchida, Shigeki Delay in hepatocyte proliferation and prostaglandin D2 synthase expression for cholestasis due to endotoxin during partial hepatectomy in rats |
title | Delay in hepatocyte proliferation and prostaglandin D2 synthase expression for cholestasis due to endotoxin during partial hepatectomy in rats |
title_full | Delay in hepatocyte proliferation and prostaglandin D2 synthase expression for cholestasis due to endotoxin during partial hepatectomy in rats |
title_fullStr | Delay in hepatocyte proliferation and prostaglandin D2 synthase expression for cholestasis due to endotoxin during partial hepatectomy in rats |
title_full_unstemmed | Delay in hepatocyte proliferation and prostaglandin D2 synthase expression for cholestasis due to endotoxin during partial hepatectomy in rats |
title_short | Delay in hepatocyte proliferation and prostaglandin D2 synthase expression for cholestasis due to endotoxin during partial hepatectomy in rats |
title_sort | delay in hepatocyte proliferation and prostaglandin d2 synthase expression for cholestasis due to endotoxin during partial hepatectomy in rats |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6797974/ https://www.ncbi.nlm.nih.gov/pubmed/31545425 http://dx.doi.org/10.3892/mmr.2019.10681 |
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