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Recombinant betatrophin (Angptl-8/lipasin) ameliorates streptozotocin-induced hyperglycemia and β-cell destruction in neonatal rats
Betatrophin [also known as lipasin, angiopoietin-like 8 (ANGPTL8), refeeding induced in fat and liver (RIFL), or hepatocellular carcinoma-associated gene TD26], a 22-kDa protein in the angiopoietin-like family, is a liver-derived hormone that promotes pancreatic β-cell proliferation and lipid metabo...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6797976/ https://www.ncbi.nlm.nih.gov/pubmed/31702044 http://dx.doi.org/10.3892/mmr.2019.10719 |
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author | Zhang, Di Yu, Yan-Juan Xu, Feng-Sen Yuan, Jun-Hua Wang, Rui Zhang, Cai-Shun Wang, Liu-Xin Liu, Yuan Song, Li-Min Liu, Jun-Li Dong, Jing |
author_facet | Zhang, Di Yu, Yan-Juan Xu, Feng-Sen Yuan, Jun-Hua Wang, Rui Zhang, Cai-Shun Wang, Liu-Xin Liu, Yuan Song, Li-Min Liu, Jun-Li Dong, Jing |
author_sort | Zhang, Di |
collection | PubMed |
description | Betatrophin [also known as lipasin, angiopoietin-like 8 (ANGPTL8), refeeding induced in fat and liver (RIFL), or hepatocellular carcinoma-associated gene TD26], a 22-kDa protein in the angiopoietin-like family, is a liver-derived hormone that promotes pancreatic β-cell proliferation and lipid metabolism. The aim of the present study was to investigate the effect of recombinant betatrophin on β-cell regeneration in a neonatal streptozotocin (STZ)-induced diabetic rat model. One-day-old Wistar rats were injected with STZ (100 mg/kg), followed by intraperitoneal administration of betatrophin to the STZ-injected rats for 6 days. Plasma glucose and body weight were monitored. On days 4 and 7, expression levels of pancreatic duodenal homeobox gene-1 (PDX-1), the Bax/B-cell lymphoma-2 (Bcl-2) ratio and plasma insulin were assessed, and the β-cell proliferation rate was determined. Pancreatic islet area and number were determined at 10 weeks. It was found that betatrophin treatment alleviated STZ-induced hyperglycemia, elevated pancreatic expression levels of Bcl-2, PDX-1, plasma insulin levels and the β-cell proliferation rate on days 4 and 7. Long-term betatrophin treatment improved glucose tolerance, associated with improved plasma insulin levels and β-cell mass. These results suggest that early administration of betatrophin promotes β-cell proliferation in STZ-induced diabetic neonates and prevents the development of diabetes in adults. |
format | Online Article Text |
id | pubmed-6797976 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-67979762019-10-22 Recombinant betatrophin (Angptl-8/lipasin) ameliorates streptozotocin-induced hyperglycemia and β-cell destruction in neonatal rats Zhang, Di Yu, Yan-Juan Xu, Feng-Sen Yuan, Jun-Hua Wang, Rui Zhang, Cai-Shun Wang, Liu-Xin Liu, Yuan Song, Li-Min Liu, Jun-Li Dong, Jing Mol Med Rep Articles Betatrophin [also known as lipasin, angiopoietin-like 8 (ANGPTL8), refeeding induced in fat and liver (RIFL), or hepatocellular carcinoma-associated gene TD26], a 22-kDa protein in the angiopoietin-like family, is a liver-derived hormone that promotes pancreatic β-cell proliferation and lipid metabolism. The aim of the present study was to investigate the effect of recombinant betatrophin on β-cell regeneration in a neonatal streptozotocin (STZ)-induced diabetic rat model. One-day-old Wistar rats were injected with STZ (100 mg/kg), followed by intraperitoneal administration of betatrophin to the STZ-injected rats for 6 days. Plasma glucose and body weight were monitored. On days 4 and 7, expression levels of pancreatic duodenal homeobox gene-1 (PDX-1), the Bax/B-cell lymphoma-2 (Bcl-2) ratio and plasma insulin were assessed, and the β-cell proliferation rate was determined. Pancreatic islet area and number were determined at 10 weeks. It was found that betatrophin treatment alleviated STZ-induced hyperglycemia, elevated pancreatic expression levels of Bcl-2, PDX-1, plasma insulin levels and the β-cell proliferation rate on days 4 and 7. Long-term betatrophin treatment improved glucose tolerance, associated with improved plasma insulin levels and β-cell mass. These results suggest that early administration of betatrophin promotes β-cell proliferation in STZ-induced diabetic neonates and prevents the development of diabetes in adults. D.A. Spandidos 2019-11 2019-10-01 /pmc/articles/PMC6797976/ /pubmed/31702044 http://dx.doi.org/10.3892/mmr.2019.10719 Text en Copyright: © Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Zhang, Di Yu, Yan-Juan Xu, Feng-Sen Yuan, Jun-Hua Wang, Rui Zhang, Cai-Shun Wang, Liu-Xin Liu, Yuan Song, Li-Min Liu, Jun-Li Dong, Jing Recombinant betatrophin (Angptl-8/lipasin) ameliorates streptozotocin-induced hyperglycemia and β-cell destruction in neonatal rats |
title | Recombinant betatrophin (Angptl-8/lipasin) ameliorates streptozotocin-induced hyperglycemia and β-cell destruction in neonatal rats |
title_full | Recombinant betatrophin (Angptl-8/lipasin) ameliorates streptozotocin-induced hyperglycemia and β-cell destruction in neonatal rats |
title_fullStr | Recombinant betatrophin (Angptl-8/lipasin) ameliorates streptozotocin-induced hyperglycemia and β-cell destruction in neonatal rats |
title_full_unstemmed | Recombinant betatrophin (Angptl-8/lipasin) ameliorates streptozotocin-induced hyperglycemia and β-cell destruction in neonatal rats |
title_short | Recombinant betatrophin (Angptl-8/lipasin) ameliorates streptozotocin-induced hyperglycemia and β-cell destruction in neonatal rats |
title_sort | recombinant betatrophin (angptl-8/lipasin) ameliorates streptozotocin-induced hyperglycemia and β-cell destruction in neonatal rats |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6797976/ https://www.ncbi.nlm.nih.gov/pubmed/31702044 http://dx.doi.org/10.3892/mmr.2019.10719 |
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