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Hybrid Polyester-Hydrogel Electrospun Scaffolds for Tissue Engineering Applications
Electrospinning is an attractive fabrication process providing a cost-effective and straightforward technic to make extra-cellular matrix (ECM) mimicking scaffolds that can be used to replace or repair injured tissues and organs. Synthetic polymers as poly (ε-caprolactone) (PCL) and poly (ethylene o...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6798037/ https://www.ncbi.nlm.nih.gov/pubmed/31681736 http://dx.doi.org/10.3389/fbioe.2019.00231 |
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author | Gonçalves de Pinho, Ana Rita Odila, Ines Leferink, Anne van Blitterswijk, Clemens Camarero-Espinosa, Sandra Moroni, Lorenzo |
author_facet | Gonçalves de Pinho, Ana Rita Odila, Ines Leferink, Anne van Blitterswijk, Clemens Camarero-Espinosa, Sandra Moroni, Lorenzo |
author_sort | Gonçalves de Pinho, Ana Rita |
collection | PubMed |
description | Electrospinning is an attractive fabrication process providing a cost-effective and straightforward technic to make extra-cellular matrix (ECM) mimicking scaffolds that can be used to replace or repair injured tissues and organs. Synthetic polymers as poly (ε-caprolactone) (PCL) and poly (ethylene oxide terephthalate)-poly(butylene terephthalate) (PEOT/PBT) have been often used to produce scaffolds due to their good processability, mechanical properties, and suitable biocompatibility. While synthetic polymers can mimic the physical features of native ECM, natural polymers like alginate are better suited to recapitulate its hydrated state or introduce functional groups that are recognized by cells (e.g., –NH(2)). Thus, this study aims at creating electrospun meshes made of blended synthetic and natural polymers for tissue engineering applications. Polyethylene oxide (PEO), PCL, and PEOT/PBT were used as a carrier of Alginate. Scaffolds were electrospun at different flow rates and distances between spinneret and collector (air gap), and the resulting meshes were characterized in terms of fiber morphology, diameter, and mesh inter-fiber pore size. The fiber diameter increased with increasing flow rate, while there was no substantial influence of the air gap. On the other hand, the mesh pore size increased with increasing air gap, while the effect of flow rate was not significant. Cross-linking and washing of alginate electrospun scaffolds resulted in smaller fiber diameter. These newly developed scaffolds may find useful applications for tissue engineering strategies as they resemble physical and chemical properties of tissue ECM. Human Dermal Fibroblasts were cultured on PCL and PCL/Alginate scaffolds in order to create a dermal substitute. |
format | Online Article Text |
id | pubmed-6798037 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-67980372019-11-01 Hybrid Polyester-Hydrogel Electrospun Scaffolds for Tissue Engineering Applications Gonçalves de Pinho, Ana Rita Odila, Ines Leferink, Anne van Blitterswijk, Clemens Camarero-Espinosa, Sandra Moroni, Lorenzo Front Bioeng Biotechnol Bioengineering and Biotechnology Electrospinning is an attractive fabrication process providing a cost-effective and straightforward technic to make extra-cellular matrix (ECM) mimicking scaffolds that can be used to replace or repair injured tissues and organs. Synthetic polymers as poly (ε-caprolactone) (PCL) and poly (ethylene oxide terephthalate)-poly(butylene terephthalate) (PEOT/PBT) have been often used to produce scaffolds due to their good processability, mechanical properties, and suitable biocompatibility. While synthetic polymers can mimic the physical features of native ECM, natural polymers like alginate are better suited to recapitulate its hydrated state or introduce functional groups that are recognized by cells (e.g., –NH(2)). Thus, this study aims at creating electrospun meshes made of blended synthetic and natural polymers for tissue engineering applications. Polyethylene oxide (PEO), PCL, and PEOT/PBT were used as a carrier of Alginate. Scaffolds were electrospun at different flow rates and distances between spinneret and collector (air gap), and the resulting meshes were characterized in terms of fiber morphology, diameter, and mesh inter-fiber pore size. The fiber diameter increased with increasing flow rate, while there was no substantial influence of the air gap. On the other hand, the mesh pore size increased with increasing air gap, while the effect of flow rate was not significant. Cross-linking and washing of alginate electrospun scaffolds resulted in smaller fiber diameter. These newly developed scaffolds may find useful applications for tissue engineering strategies as they resemble physical and chemical properties of tissue ECM. Human Dermal Fibroblasts were cultured on PCL and PCL/Alginate scaffolds in order to create a dermal substitute. Frontiers Media S.A. 2019-09-25 /pmc/articles/PMC6798037/ /pubmed/31681736 http://dx.doi.org/10.3389/fbioe.2019.00231 Text en Copyright © 2019 Gonçalves de Pinho, Odila, Leferink, van Blitterswijk, Camarero-Espinosa and Moroni. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Gonçalves de Pinho, Ana Rita Odila, Ines Leferink, Anne van Blitterswijk, Clemens Camarero-Espinosa, Sandra Moroni, Lorenzo Hybrid Polyester-Hydrogel Electrospun Scaffolds for Tissue Engineering Applications |
title | Hybrid Polyester-Hydrogel Electrospun Scaffolds for Tissue Engineering Applications |
title_full | Hybrid Polyester-Hydrogel Electrospun Scaffolds for Tissue Engineering Applications |
title_fullStr | Hybrid Polyester-Hydrogel Electrospun Scaffolds for Tissue Engineering Applications |
title_full_unstemmed | Hybrid Polyester-Hydrogel Electrospun Scaffolds for Tissue Engineering Applications |
title_short | Hybrid Polyester-Hydrogel Electrospun Scaffolds for Tissue Engineering Applications |
title_sort | hybrid polyester-hydrogel electrospun scaffolds for tissue engineering applications |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6798037/ https://www.ncbi.nlm.nih.gov/pubmed/31681736 http://dx.doi.org/10.3389/fbioe.2019.00231 |
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