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IgA and IgG antibody detection of mycobacterial antigens in pleural fluid and serum from pleural tuberculous patients
BACKGROUND: A previous study demonstrated pleural fluid (PF) IgA immunodominance for the fused MT10.3:MPT64 protein in pleural tuberculosis (PLTB) cases. However, no clue on the role of IgA and IgG against this and other antigens in PF and serum concerning improved diagnosis is available. Thus, the...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6798396/ https://www.ncbi.nlm.nih.gov/pubmed/31623558 http://dx.doi.org/10.1186/s12865-019-0315-y |
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author | da Silva, Renan Jeremias da Silva Corrêa, Raquel Sardella, Isabela Gama de Paulo Mulinari, Ana Carla Mafort, Thiago Thomaz Santos, Ana Paula Rufino, Rogério Rodrigues, Luciana Silva Saad, Maria Helena Féres |
author_facet | da Silva, Renan Jeremias da Silva Corrêa, Raquel Sardella, Isabela Gama de Paulo Mulinari, Ana Carla Mafort, Thiago Thomaz Santos, Ana Paula Rufino, Rogério Rodrigues, Luciana Silva Saad, Maria Helena Féres |
author_sort | da Silva, Renan Jeremias |
collection | PubMed |
description | BACKGROUND: A previous study demonstrated pleural fluid (PF) IgA immunodominance for the fused MT10.3:MPT64 protein in pleural tuberculosis (PLTB) cases. However, no clue on the role of IgA and IgG against this and other antigens in PF and serum concerning improved diagnosis is available. Thus, the aim of the present study was to validate PF IgA-MT10.3:MPT64 and evaluate PF and serum IgA and IgG reactivity against this protein, its peptides (F2) and single MPT64, MT10.3 and the PPE59 mycobacterial specific antigens. IgA and IgG ELISA were measured against the antigen in PLTB (n = 29) and other non-TB pleurisy (n = 39) patient samples. RESULTS: The immunodominance of PF IgA-MT10.3:MPT64 was confirmed in PLTB (86.2%) followed by PPE59 (62%), while serum IgA-F2 exhibited 51.7% sensitivity. PF and serum IgG-MT10.3:MPT64 led to 65.5 and 51.7% sensitivity, respectively. However, MT10.3 and MPT64 displayed overall lower sensitivity (≤34.5) for both antibodies. All results at 95% fixed specificity. Combinatory results indicated 93.1% sensitivity for PF IgA-MT10.3:MPT64/−PPE59 and IgA/IgG-MT10.3:MPT64 at 92.3% specificity, followed by IgA-MT10.3:MPT64/−MPT64 or /−F2 (89.6%) without jeopardizing specificity (94.9%). The combinatory results of the PF adenosine deaminase test (ADA) and IgA-MT10.3:MPT64/−F2 demonstrated the highest sensitivity (96.6%), with a specificity of 92.3%. CONCLUSIONS: The PF IgA-MT10:MPT64 immune dominance was validated in PLTB, and its combinatory results with PPE59 or MPT64 or F2 antigens as well as with IgG, are reported herein for the first time, improving their potential to assist diagnosis. Combining PF-ADA and IgA-MT10.3:MPT64/−F2 results achieved better accuracy. Moreover, serum IgG, although less accurate, displays potential beyond microbiological tests. |
format | Online Article Text |
id | pubmed-6798396 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-67983962019-10-21 IgA and IgG antibody detection of mycobacterial antigens in pleural fluid and serum from pleural tuberculous patients da Silva, Renan Jeremias da Silva Corrêa, Raquel Sardella, Isabela Gama de Paulo Mulinari, Ana Carla Mafort, Thiago Thomaz Santos, Ana Paula Rufino, Rogério Rodrigues, Luciana Silva Saad, Maria Helena Féres BMC Immunol Research Article BACKGROUND: A previous study demonstrated pleural fluid (PF) IgA immunodominance for the fused MT10.3:MPT64 protein in pleural tuberculosis (PLTB) cases. However, no clue on the role of IgA and IgG against this and other antigens in PF and serum concerning improved diagnosis is available. Thus, the aim of the present study was to validate PF IgA-MT10.3:MPT64 and evaluate PF and serum IgA and IgG reactivity against this protein, its peptides (F2) and single MPT64, MT10.3 and the PPE59 mycobacterial specific antigens. IgA and IgG ELISA were measured against the antigen in PLTB (n = 29) and other non-TB pleurisy (n = 39) patient samples. RESULTS: The immunodominance of PF IgA-MT10.3:MPT64 was confirmed in PLTB (86.2%) followed by PPE59 (62%), while serum IgA-F2 exhibited 51.7% sensitivity. PF and serum IgG-MT10.3:MPT64 led to 65.5 and 51.7% sensitivity, respectively. However, MT10.3 and MPT64 displayed overall lower sensitivity (≤34.5) for both antibodies. All results at 95% fixed specificity. Combinatory results indicated 93.1% sensitivity for PF IgA-MT10.3:MPT64/−PPE59 and IgA/IgG-MT10.3:MPT64 at 92.3% specificity, followed by IgA-MT10.3:MPT64/−MPT64 or /−F2 (89.6%) without jeopardizing specificity (94.9%). The combinatory results of the PF adenosine deaminase test (ADA) and IgA-MT10.3:MPT64/−F2 demonstrated the highest sensitivity (96.6%), with a specificity of 92.3%. CONCLUSIONS: The PF IgA-MT10:MPT64 immune dominance was validated in PLTB, and its combinatory results with PPE59 or MPT64 or F2 antigens as well as with IgG, are reported herein for the first time, improving their potential to assist diagnosis. Combining PF-ADA and IgA-MT10.3:MPT64/−F2 results achieved better accuracy. Moreover, serum IgG, although less accurate, displays potential beyond microbiological tests. BioMed Central 2019-10-17 /pmc/articles/PMC6798396/ /pubmed/31623558 http://dx.doi.org/10.1186/s12865-019-0315-y Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article da Silva, Renan Jeremias da Silva Corrêa, Raquel Sardella, Isabela Gama de Paulo Mulinari, Ana Carla Mafort, Thiago Thomaz Santos, Ana Paula Rufino, Rogério Rodrigues, Luciana Silva Saad, Maria Helena Féres IgA and IgG antibody detection of mycobacterial antigens in pleural fluid and serum from pleural tuberculous patients |
title | IgA and IgG antibody detection of mycobacterial antigens in pleural fluid and serum from pleural tuberculous patients |
title_full | IgA and IgG antibody detection of mycobacterial antigens in pleural fluid and serum from pleural tuberculous patients |
title_fullStr | IgA and IgG antibody detection of mycobacterial antigens in pleural fluid and serum from pleural tuberculous patients |
title_full_unstemmed | IgA and IgG antibody detection of mycobacterial antigens in pleural fluid and serum from pleural tuberculous patients |
title_short | IgA and IgG antibody detection of mycobacterial antigens in pleural fluid and serum from pleural tuberculous patients |
title_sort | iga and igg antibody detection of mycobacterial antigens in pleural fluid and serum from pleural tuberculous patients |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6798396/ https://www.ncbi.nlm.nih.gov/pubmed/31623558 http://dx.doi.org/10.1186/s12865-019-0315-y |
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