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Osteogenic potential of induced pluripotent stem cells from human adipose-derived stem cells

BACKGROUND: Bone regeneration is a crucial and challenging issue in clinical practice. Bone tissue engineering (BTE) with an optimal cell source may provide an ideal strategy for the reconstruction of bone defects. This study examined whether induced pluripotent stem cells (iPSCs) derived from adipo...

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Autores principales: Mao, Shih-Hsuan, Chen, Chih-Hao, Chen, Chien-Tzung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6798413/
https://www.ncbi.nlm.nih.gov/pubmed/31623672
http://dx.doi.org/10.1186/s13287-019-1402-y
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author Mao, Shih-Hsuan
Chen, Chih-Hao
Chen, Chien-Tzung
author_facet Mao, Shih-Hsuan
Chen, Chih-Hao
Chen, Chien-Tzung
author_sort Mao, Shih-Hsuan
collection PubMed
description BACKGROUND: Bone regeneration is a crucial and challenging issue in clinical practice. Bone tissue engineering (BTE) with an optimal cell source may provide an ideal strategy for the reconstruction of bone defects. This study examined whether induced pluripotent stem cells (iPSCs) derived from adipose-derived stem cells (ASCs) could act as an osteogenic substitute and whether these ASC-iPSCs yield more new bone formation than ASCs in hydrogel scaffolds. METHODS: ASC-iPSCs were reprogrammed from ASCs through a retroviral system. ASCs were harvested and isolated from adipose tissue of humans. An aliquot of cell suspension (1 × 10(6) cells/mL) was seeded directly onto the nHAP-gelatin cryogel scaffolds. Nude mice back implantation of cell-seeded scaffolds was designed for in vivo comparison of osteogenic potentials between ASCs and ASC-iPSCs. Samples were harvested 4 and 8 weeks after implantation for further analysis based on histology and RT-PCR. RESULTS: ASC-iPSCs were successfully obtained from human adipose-derived stem cells. PCR results also showed that specific genes of iPSCs with the ability to cause the differentiation of cells into the three germ layers were expressed. In our in vivo experiments, iPSCs were subcutaneously injected into nude mice to induce teratoma formation. The morphology of the three germ layers was confirmed by histological staining. ASC is an essential cell source for BTE with benefits of high volume and less-invasive acquisition. With additional transforming Yamanaka factors, ASC-iPSCs showed higher osteogenic differentiation and elevated expression of collagen type I (Col I), osteocalcin (OCN), alkaline phosphate (ALP), and runt-related transcription factor 2 (RunX-2). CONCLUSIONS: This report suggests that ASC-iPSCs could be a superior cell source in BTE with better osteogenic differentiation efficacy for future clinical applications.
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spelling pubmed-67984132019-10-21 Osteogenic potential of induced pluripotent stem cells from human adipose-derived stem cells Mao, Shih-Hsuan Chen, Chih-Hao Chen, Chien-Tzung Stem Cell Res Ther Research BACKGROUND: Bone regeneration is a crucial and challenging issue in clinical practice. Bone tissue engineering (BTE) with an optimal cell source may provide an ideal strategy for the reconstruction of bone defects. This study examined whether induced pluripotent stem cells (iPSCs) derived from adipose-derived stem cells (ASCs) could act as an osteogenic substitute and whether these ASC-iPSCs yield more new bone formation than ASCs in hydrogel scaffolds. METHODS: ASC-iPSCs were reprogrammed from ASCs through a retroviral system. ASCs were harvested and isolated from adipose tissue of humans. An aliquot of cell suspension (1 × 10(6) cells/mL) was seeded directly onto the nHAP-gelatin cryogel scaffolds. Nude mice back implantation of cell-seeded scaffolds was designed for in vivo comparison of osteogenic potentials between ASCs and ASC-iPSCs. Samples were harvested 4 and 8 weeks after implantation for further analysis based on histology and RT-PCR. RESULTS: ASC-iPSCs were successfully obtained from human adipose-derived stem cells. PCR results also showed that specific genes of iPSCs with the ability to cause the differentiation of cells into the three germ layers were expressed. In our in vivo experiments, iPSCs were subcutaneously injected into nude mice to induce teratoma formation. The morphology of the three germ layers was confirmed by histological staining. ASC is an essential cell source for BTE with benefits of high volume and less-invasive acquisition. With additional transforming Yamanaka factors, ASC-iPSCs showed higher osteogenic differentiation and elevated expression of collagen type I (Col I), osteocalcin (OCN), alkaline phosphate (ALP), and runt-related transcription factor 2 (RunX-2). CONCLUSIONS: This report suggests that ASC-iPSCs could be a superior cell source in BTE with better osteogenic differentiation efficacy for future clinical applications. BioMed Central 2019-10-17 /pmc/articles/PMC6798413/ /pubmed/31623672 http://dx.doi.org/10.1186/s13287-019-1402-y Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Mao, Shih-Hsuan
Chen, Chih-Hao
Chen, Chien-Tzung
Osteogenic potential of induced pluripotent stem cells from human adipose-derived stem cells
title Osteogenic potential of induced pluripotent stem cells from human adipose-derived stem cells
title_full Osteogenic potential of induced pluripotent stem cells from human adipose-derived stem cells
title_fullStr Osteogenic potential of induced pluripotent stem cells from human adipose-derived stem cells
title_full_unstemmed Osteogenic potential of induced pluripotent stem cells from human adipose-derived stem cells
title_short Osteogenic potential of induced pluripotent stem cells from human adipose-derived stem cells
title_sort osteogenic potential of induced pluripotent stem cells from human adipose-derived stem cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6798413/
https://www.ncbi.nlm.nih.gov/pubmed/31623672
http://dx.doi.org/10.1186/s13287-019-1402-y
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