Cargando…
Clomiphene citrate ameliorated lead acetate-induced reproductive toxicity in male Wistar rats
OBJECTIVE: The current study investigated the effects of clomiphene citrate on the hypothalamic-pituitary-testicular axis, steroidogenesis, sperm parameters, and testicular antioxidant enzyme activity of male Wistar rats submitted to lead acetate (Pb)-induced reproductive toxicity. METHODS: Twenty a...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Brazilian Society of Assisted Reproduction
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6798595/ https://www.ncbi.nlm.nih.gov/pubmed/31173495 http://dx.doi.org/10.5935/1518-0557.20190038 |
_version_ | 1783460079891120128 |
---|---|
author | Wahab, Oyeyemi A. Princely, Anyanwu C. Oluwadamilare, Akinola A. Oore-oluwapo, Daramola O. Blessing, Alli O. Alfred, Ehiaghe F. |
author_facet | Wahab, Oyeyemi A. Princely, Anyanwu C. Oluwadamilare, Akinola A. Oore-oluwapo, Daramola O. Blessing, Alli O. Alfred, Ehiaghe F. |
author_sort | Wahab, Oyeyemi A. |
collection | PubMed |
description | OBJECTIVE: The current study investigated the effects of clomiphene citrate on the hypothalamic-pituitary-testicular axis, steroidogenesis, sperm parameters, and testicular antioxidant enzyme activity of male Wistar rats submitted to lead acetate (Pb)-induced reproductive toxicity. METHODS: Twenty adult male Wistar rats were divided into four groups of equal size as follows: Control; Clomid (0.35 mg/kg); Pb (10 mg/kg); and Clomid + Pb. Serum levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), testosterone, testicular 17-β hydroxysteroid dehydrogenase (17-β HSD) activity, androgen receptors, catalase activity, superoxide dismutase (SOD), malondialdehyde (MDA), sperm motility, viability, counts and morphology were estimated after oral administration of Clomid and/or lead acetate for 35 consecutive days. Data were analyzed using ANOVA at p<0.05. RESULTS: Lead acetate significantly decreased (p<0.05) serum LH and testosterone levels, testicular 17β-HSD activity, androgen receptor expression, sperm motility, viability, counts, catalase activity, and SOD when compared with controls. Abnormal sperm morphology and MDA were significantly increased (p<0.05) in the Pb group compared with controls. Clomid co-administrated with lead acetate significantly increased (p<0.05) serum LH, testosterone levels, testicular 17β-HSD, androgen receptor expression, sperm motility and viability when compared with the group given lead acetate. CONCLUSIONS: The present study suggests that clomiphene citrate may stimulate testicular testosterone synthesis, sperm motility and viability via luteinizing hormone in a context of lead acetate-induced reproductive toxicity. |
format | Online Article Text |
id | pubmed-6798595 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Brazilian Society of Assisted Reproduction |
record_format | MEDLINE/PubMed |
spelling | pubmed-67985952019-10-22 Clomiphene citrate ameliorated lead acetate-induced reproductive toxicity in male Wistar rats Wahab, Oyeyemi A. Princely, Anyanwu C. Oluwadamilare, Akinola A. Oore-oluwapo, Daramola O. Blessing, Alli O. Alfred, Ehiaghe F. JBRA Assist Reprod Original Article OBJECTIVE: The current study investigated the effects of clomiphene citrate on the hypothalamic-pituitary-testicular axis, steroidogenesis, sperm parameters, and testicular antioxidant enzyme activity of male Wistar rats submitted to lead acetate (Pb)-induced reproductive toxicity. METHODS: Twenty adult male Wistar rats were divided into four groups of equal size as follows: Control; Clomid (0.35 mg/kg); Pb (10 mg/kg); and Clomid + Pb. Serum levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), testosterone, testicular 17-β hydroxysteroid dehydrogenase (17-β HSD) activity, androgen receptors, catalase activity, superoxide dismutase (SOD), malondialdehyde (MDA), sperm motility, viability, counts and morphology were estimated after oral administration of Clomid and/or lead acetate for 35 consecutive days. Data were analyzed using ANOVA at p<0.05. RESULTS: Lead acetate significantly decreased (p<0.05) serum LH and testosterone levels, testicular 17β-HSD activity, androgen receptor expression, sperm motility, viability, counts, catalase activity, and SOD when compared with controls. Abnormal sperm morphology and MDA were significantly increased (p<0.05) in the Pb group compared with controls. Clomid co-administrated with lead acetate significantly increased (p<0.05) serum LH, testosterone levels, testicular 17β-HSD, androgen receptor expression, sperm motility and viability when compared with the group given lead acetate. CONCLUSIONS: The present study suggests that clomiphene citrate may stimulate testicular testosterone synthesis, sperm motility and viability via luteinizing hormone in a context of lead acetate-induced reproductive toxicity. Brazilian Society of Assisted Reproduction 2019 /pmc/articles/PMC6798595/ /pubmed/31173495 http://dx.doi.org/10.5935/1518-0557.20190038 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Wahab, Oyeyemi A. Princely, Anyanwu C. Oluwadamilare, Akinola A. Oore-oluwapo, Daramola O. Blessing, Alli O. Alfred, Ehiaghe F. Clomiphene citrate ameliorated lead acetate-induced reproductive toxicity in male Wistar rats |
title | Clomiphene citrate ameliorated lead acetate-induced reproductive
toxicity in male Wistar rats |
title_full | Clomiphene citrate ameliorated lead acetate-induced reproductive
toxicity in male Wistar rats |
title_fullStr | Clomiphene citrate ameliorated lead acetate-induced reproductive
toxicity in male Wistar rats |
title_full_unstemmed | Clomiphene citrate ameliorated lead acetate-induced reproductive
toxicity in male Wistar rats |
title_short | Clomiphene citrate ameliorated lead acetate-induced reproductive
toxicity in male Wistar rats |
title_sort | clomiphene citrate ameliorated lead acetate-induced reproductive
toxicity in male wistar rats |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6798595/ https://www.ncbi.nlm.nih.gov/pubmed/31173495 http://dx.doi.org/10.5935/1518-0557.20190038 |
work_keys_str_mv | AT wahaboyeyemia clomiphenecitrateamelioratedleadacetateinducedreproductivetoxicityinmalewistarrats AT princelyanyanwuc clomiphenecitrateamelioratedleadacetateinducedreproductivetoxicityinmalewistarrats AT oluwadamilareakinolaa clomiphenecitrateamelioratedleadacetateinducedreproductivetoxicityinmalewistarrats AT ooreoluwapodaramolao clomiphenecitrateamelioratedleadacetateinducedreproductivetoxicityinmalewistarrats AT blessingallio clomiphenecitrateamelioratedleadacetateinducedreproductivetoxicityinmalewistarrats AT alfredehiaghef clomiphenecitrateamelioratedleadacetateinducedreproductivetoxicityinmalewistarrats |