2508: The role of platelet factor-4 (PF4 or CXCL4) in B cell differentiation

OBJECTIVES/SPECIFIC AIMS: To investigate the role of platelet factor-4 (PF4) in B cell differentiation and develop strategies to better modulate B cell differentiation in vitro and in vivo. METHODS/STUDY POPULATION: We use tissue culture and flow cytometry to examine the role of PF4 in B cell differ...

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Detalles Bibliográficos
Autores principales: Blick, Sara, Morrell, Craig, Ture, Sara, Field, David J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cambridge University Press 2018
Materias:
Basic Science/Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6799014/
http://dx.doi.org/10.1017/cts.2017.52
Descripción
Sumario:OBJECTIVES/SPECIFIC AIMS: To investigate the role of platelet factor-4 (PF4) in B cell differentiation and develop strategies to better modulate B cell differentiation in vitro and in vivo. METHODS/STUDY POPULATION: We use tissue culture and flow cytometry to examine the role of PF4 in B cell differentiation. We use wild type (WT) and PF4(−/−) mice on a C57Bl6/J background. PF4(−/−) mice have reduced in vivo B cell differentiation responses. RESULTS/ANTICIPATED RESULTS: We anticipate that our studies will demonstrate that PF4 promotes B cell differentiation in the bone marrow microenvironment. DISCUSSION/SIGNIFICANCE OF IMPACT: The significance of this project may be valuable in developing efficient methods and strategies to increase or limit B cell numbers in vitro and in human disease.

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