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Longitudinal Intra- and Inter-individual variation in T-cell subsets of HIV-infected and uninfected men participating in the LA Multi-Center AIDS Cohort Study
To assess the intra-individual and inter-individuals biological variation and the effect of aging on lymphocyte T-cells subsets. We assessed lymphocyte phenotypes (CD3(+), CD4(+), and CD8(+) T-cells) in 89 HIV-1-infected and 88 uninfected white non-Hispanic men every 6 months, to examine the biologi...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer Health
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6799419/ https://www.ncbi.nlm.nih.gov/pubmed/31593126 http://dx.doi.org/10.1097/MD.0000000000017525 |
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author | Aziz, Najib Jamieson, Beth D. Quint, Joshua J. Martinez-Maza, Otoniel Chow, Marianne Detels, Roger |
author_facet | Aziz, Najib Jamieson, Beth D. Quint, Joshua J. Martinez-Maza, Otoniel Chow, Marianne Detels, Roger |
author_sort | Aziz, Najib |
collection | PubMed |
description | To assess the intra-individual and inter-individuals biological variation and the effect of aging on lymphocyte T-cells subsets. We assessed lymphocyte phenotypes (CD3(+), CD4(+), and CD8(+) T-cells) in 89 HIV-1-infected and 88 uninfected white non-Hispanic men every 6 months, to examine the biological variation for those measurements, and the average change in lymphocyte phenotype over 34 years. The markers showed significant intra-individuality in HIV-infected and uninfected individuals with index of individuality of <1.4. No mean changes were seen over the 34 years, with the exception of percentage CD4(+)T-cells in HIV-uninfected individuals. In the pre-HAART era, HIV-infected individuals experienced an increase in mean absolute CD3(+) T-cell numbers (11.21 cells/μL, P = 0.02) and absolute CD8(+) T-cell numbers (34.57 cell/μl, P < .001), and in the percentage of CD8(+) T-cells (1.45%, P < .001) per year and a significant decrease in mean absolute CD4(+) T-cell numbers (23.68 cells/μl, P < .001) and in the percentage of CD4(+) T-cells (1.49%, P < .001) per year. In the post-HAART era, no changes in mean levels were observed in absolute CD3(+) T-cell count (P = .15) or percentage (P = .99). Significant decreases were seen in mean count (8.56 cells/μl, P < .001) and percentage (0.59%, P < .001) of CD8(+) T-cells, and increases in mean absolute count (10.72 cells/μl, P < .001) and percentage (0.47%, P < .001) of CD4(+) T-cells. With the exception of CD4 (%), no average changes per year were seen in lymphocyte phenotype of HIV-uninfected men. The results of coefficients of variation of intra and inter-individuals of this study can be useful for HIV-1 infection monitoring and in addition the observation could be a useful guide for intra- and inter-individual coefficient variations, and establishing quality goal studies of different blood biomarkers in healthy and other diseases. |
format | Online Article Text |
id | pubmed-6799419 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Wolters Kluwer Health |
record_format | MEDLINE/PubMed |
spelling | pubmed-67994192019-11-18 Longitudinal Intra- and Inter-individual variation in T-cell subsets of HIV-infected and uninfected men participating in the LA Multi-Center AIDS Cohort Study Aziz, Najib Jamieson, Beth D. Quint, Joshua J. Martinez-Maza, Otoniel Chow, Marianne Detels, Roger Medicine (Baltimore) 4850 To assess the intra-individual and inter-individuals biological variation and the effect of aging on lymphocyte T-cells subsets. We assessed lymphocyte phenotypes (CD3(+), CD4(+), and CD8(+) T-cells) in 89 HIV-1-infected and 88 uninfected white non-Hispanic men every 6 months, to examine the biological variation for those measurements, and the average change in lymphocyte phenotype over 34 years. The markers showed significant intra-individuality in HIV-infected and uninfected individuals with index of individuality of <1.4. No mean changes were seen over the 34 years, with the exception of percentage CD4(+)T-cells in HIV-uninfected individuals. In the pre-HAART era, HIV-infected individuals experienced an increase in mean absolute CD3(+) T-cell numbers (11.21 cells/μL, P = 0.02) and absolute CD8(+) T-cell numbers (34.57 cell/μl, P < .001), and in the percentage of CD8(+) T-cells (1.45%, P < .001) per year and a significant decrease in mean absolute CD4(+) T-cell numbers (23.68 cells/μl, P < .001) and in the percentage of CD4(+) T-cells (1.49%, P < .001) per year. In the post-HAART era, no changes in mean levels were observed in absolute CD3(+) T-cell count (P = .15) or percentage (P = .99). Significant decreases were seen in mean count (8.56 cells/μl, P < .001) and percentage (0.59%, P < .001) of CD8(+) T-cells, and increases in mean absolute count (10.72 cells/μl, P < .001) and percentage (0.47%, P < .001) of CD4(+) T-cells. With the exception of CD4 (%), no average changes per year were seen in lymphocyte phenotype of HIV-uninfected men. The results of coefficients of variation of intra and inter-individuals of this study can be useful for HIV-1 infection monitoring and in addition the observation could be a useful guide for intra- and inter-individual coefficient variations, and establishing quality goal studies of different blood biomarkers in healthy and other diseases. Wolters Kluwer Health 2019-10-11 /pmc/articles/PMC6799419/ /pubmed/31593126 http://dx.doi.org/10.1097/MD.0000000000017525 Text en Copyright © 2019 the Author(s). Published by Wolters Kluwer Health, Inc. http://creativecommons.org/licenses/by/4.0 This is an open access article distributed under the Creative Commons Attribution License 4.0 (CCBY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by/4.0 |
spellingShingle | 4850 Aziz, Najib Jamieson, Beth D. Quint, Joshua J. Martinez-Maza, Otoniel Chow, Marianne Detels, Roger Longitudinal Intra- and Inter-individual variation in T-cell subsets of HIV-infected and uninfected men participating in the LA Multi-Center AIDS Cohort Study |
title | Longitudinal Intra- and Inter-individual variation in T-cell subsets of HIV-infected and uninfected men participating in the LA Multi-Center AIDS Cohort Study |
title_full | Longitudinal Intra- and Inter-individual variation in T-cell subsets of HIV-infected and uninfected men participating in the LA Multi-Center AIDS Cohort Study |
title_fullStr | Longitudinal Intra- and Inter-individual variation in T-cell subsets of HIV-infected and uninfected men participating in the LA Multi-Center AIDS Cohort Study |
title_full_unstemmed | Longitudinal Intra- and Inter-individual variation in T-cell subsets of HIV-infected and uninfected men participating in the LA Multi-Center AIDS Cohort Study |
title_short | Longitudinal Intra- and Inter-individual variation in T-cell subsets of HIV-infected and uninfected men participating in the LA Multi-Center AIDS Cohort Study |
title_sort | longitudinal intra- and inter-individual variation in t-cell subsets of hiv-infected and uninfected men participating in the la multi-center aids cohort study |
topic | 4850 |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6799419/ https://www.ncbi.nlm.nih.gov/pubmed/31593126 http://dx.doi.org/10.1097/MD.0000000000017525 |
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