Cargando…

Quantifying Spirorchiid Eggs in Splenic Histological Samples from Green Turtles

The present study proposes a new methodology for the quantification of parasite eggs in animal tissue. Quantification of parasites are important to understand epidemiology of spirorchiid infections in sea turtles, however different methodologies for quantifying Spirorchiidae eggs in turtle tissues h...

Descripción completa

Detalles Bibliográficos
Autores principales: D'azeredo, F., Meira-Filho, M., Work, T. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sciendo 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6799573/
https://www.ncbi.nlm.nih.gov/pubmed/31662700
http://dx.doi.org/10.2478/helm-2019-0020
_version_ 1783460316737175552
author D'azeredo, F.
Meira-Filho, M.
Work, T. M.
author_facet D'azeredo, F.
Meira-Filho, M.
Work, T. M.
author_sort D'azeredo, F.
collection PubMed
description The present study proposes a new methodology for the quantification of parasite eggs in animal tissue. Quantification of parasites are important to understand epidemiology of spirorchiid infections in sea turtles, however different methodologies for quantifying Spirorchiidae eggs in turtle tissues have been used. The most representative way to quantify Spirorchiidae burdens in tissues is counting eggs / g of tissue, however, this method is very laborious. As an alternative, we propose quantifying number of Spirorchiidae eggs/ area of tissue on a microscope slide. We compared this method to number of eggs / slide, a common metric of egg burden in turtle tissues. Both methods correlated well with eggs / g with eggs/mm(2) of tissue having better correlation.
format Online
Article
Text
id pubmed-6799573
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Sciendo
record_format MEDLINE/PubMed
spelling pubmed-67995732019-10-29 Quantifying Spirorchiid Eggs in Splenic Histological Samples from Green Turtles D'azeredo, F. Meira-Filho, M. Work, T. M. Helminthologia Research Note The present study proposes a new methodology for the quantification of parasite eggs in animal tissue. Quantification of parasites are important to understand epidemiology of spirorchiid infections in sea turtles, however different methodologies for quantifying Spirorchiidae eggs in turtle tissues have been used. The most representative way to quantify Spirorchiidae burdens in tissues is counting eggs / g of tissue, however, this method is very laborious. As an alternative, we propose quantifying number of Spirorchiidae eggs/ area of tissue on a microscope slide. We compared this method to number of eggs / slide, a common metric of egg burden in turtle tissues. Both methods correlated well with eggs / g with eggs/mm(2) of tissue having better correlation. Sciendo 2019-09-01 /pmc/articles/PMC6799573/ /pubmed/31662700 http://dx.doi.org/10.2478/helm-2019-0020 Text en © 2019 F. D´azeredo, M. Meira-Filho, T. M. Work, published by Sciendo http://creativecommons.org/licenses/by-nc-nd/4.0 This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.
spellingShingle Research Note
D'azeredo, F.
Meira-Filho, M.
Work, T. M.
Quantifying Spirorchiid Eggs in Splenic Histological Samples from Green Turtles
title Quantifying Spirorchiid Eggs in Splenic Histological Samples from Green Turtles
title_full Quantifying Spirorchiid Eggs in Splenic Histological Samples from Green Turtles
title_fullStr Quantifying Spirorchiid Eggs in Splenic Histological Samples from Green Turtles
title_full_unstemmed Quantifying Spirorchiid Eggs in Splenic Histological Samples from Green Turtles
title_short Quantifying Spirorchiid Eggs in Splenic Histological Samples from Green Turtles
title_sort quantifying spirorchiid eggs in splenic histological samples from green turtles
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6799573/
https://www.ncbi.nlm.nih.gov/pubmed/31662700
http://dx.doi.org/10.2478/helm-2019-0020
work_keys_str_mv AT dazeredof quantifyingspirorchiideggsinsplenichistologicalsamplesfromgreenturtles
AT meirafilhom quantifyingspirorchiideggsinsplenichistologicalsamplesfromgreenturtles
AT worktm quantifyingspirorchiideggsinsplenichistologicalsamplesfromgreenturtles