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A MEMS ultrasound stimulation system for modulation of neural circuits with high spatial resolution in vitro

Neuromodulation by ultrasound has recently received attention due to its noninvasive stimulation capability for treating brain diseases. Although there have been several studies related to ultrasonic neuromodulation, these studies have suffered from poor spatial resolution of the ultrasound and low...

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Autores principales: Lee, Jungpyo, Ko, Kyungmin, Shin, Hyogeun, Oh, Soo-Jin, Lee, C. Justin, Chou, Namsun, Choi, Nakwon, Tack Oh, Min, Chul Lee, Byung, Chan Jun, Seong, Cho, Il-Joo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6799809/
https://www.ncbi.nlm.nih.gov/pubmed/31636922
http://dx.doi.org/10.1038/s41378-019-0070-5
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author Lee, Jungpyo
Ko, Kyungmin
Shin, Hyogeun
Oh, Soo-Jin
Lee, C. Justin
Chou, Namsun
Choi, Nakwon
Tack Oh, Min
Chul Lee, Byung
Chan Jun, Seong
Cho, Il-Joo
author_facet Lee, Jungpyo
Ko, Kyungmin
Shin, Hyogeun
Oh, Soo-Jin
Lee, C. Justin
Chou, Namsun
Choi, Nakwon
Tack Oh, Min
Chul Lee, Byung
Chan Jun, Seong
Cho, Il-Joo
author_sort Lee, Jungpyo
collection PubMed
description Neuromodulation by ultrasound has recently received attention due to its noninvasive stimulation capability for treating brain diseases. Although there have been several studies related to ultrasonic neuromodulation, these studies have suffered from poor spatial resolution of the ultrasound and low repeatability with a fixed condition caused by conventional and commercialized ultrasound transducers. In addition, the underlying physics and mechanisms of ultrasonic neuromodulation are still unknown. To determine these mechanisms and accurately modulate neural circuits, researchers must have a precisely controllable ultrasound transducer to conduct experiments at the cellular level. Herein, we introduce a new MEMS ultrasound stimulation system for modulating neurons or brain slices with high spatial resolution. The piezoelectric micromachined ultrasonic transducers (pMUTs) with small membranes (sub-mm membranes) generate enough power to stimulate neurons and enable precise modulation of neural circuits. We designed the ultrasound transducer as an array structure to enable localized modulation in the target region. In addition, we integrated a cell culture chamber with the system to make it compatible with conventional cell-based experiments, such as in vitro cell cultures and brain slices. In this work, we successfully demonstrated the functionality of the system by showing that the number of responding cells is proportional to the acoustic intensity of the applied ultrasound. We also demonstrated localized stimulation capability with high spatial resolution by conducting experiments in which cocultured cells responded only around a working transducer.
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spelling pubmed-67998092019-10-21 A MEMS ultrasound stimulation system for modulation of neural circuits with high spatial resolution in vitro Lee, Jungpyo Ko, Kyungmin Shin, Hyogeun Oh, Soo-Jin Lee, C. Justin Chou, Namsun Choi, Nakwon Tack Oh, Min Chul Lee, Byung Chan Jun, Seong Cho, Il-Joo Microsyst Nanoeng Article Neuromodulation by ultrasound has recently received attention due to its noninvasive stimulation capability for treating brain diseases. Although there have been several studies related to ultrasonic neuromodulation, these studies have suffered from poor spatial resolution of the ultrasound and low repeatability with a fixed condition caused by conventional and commercialized ultrasound transducers. In addition, the underlying physics and mechanisms of ultrasonic neuromodulation are still unknown. To determine these mechanisms and accurately modulate neural circuits, researchers must have a precisely controllable ultrasound transducer to conduct experiments at the cellular level. Herein, we introduce a new MEMS ultrasound stimulation system for modulating neurons or brain slices with high spatial resolution. The piezoelectric micromachined ultrasonic transducers (pMUTs) with small membranes (sub-mm membranes) generate enough power to stimulate neurons and enable precise modulation of neural circuits. We designed the ultrasound transducer as an array structure to enable localized modulation in the target region. In addition, we integrated a cell culture chamber with the system to make it compatible with conventional cell-based experiments, such as in vitro cell cultures and brain slices. In this work, we successfully demonstrated the functionality of the system by showing that the number of responding cells is proportional to the acoustic intensity of the applied ultrasound. We also demonstrated localized stimulation capability with high spatial resolution by conducting experiments in which cocultured cells responded only around a working transducer. Nature Publishing Group UK 2019-07-15 /pmc/articles/PMC6799809/ /pubmed/31636922 http://dx.doi.org/10.1038/s41378-019-0070-5 Text en © The Author(s) 2019 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Lee, Jungpyo
Ko, Kyungmin
Shin, Hyogeun
Oh, Soo-Jin
Lee, C. Justin
Chou, Namsun
Choi, Nakwon
Tack Oh, Min
Chul Lee, Byung
Chan Jun, Seong
Cho, Il-Joo
A MEMS ultrasound stimulation system for modulation of neural circuits with high spatial resolution in vitro
title A MEMS ultrasound stimulation system for modulation of neural circuits with high spatial resolution in vitro
title_full A MEMS ultrasound stimulation system for modulation of neural circuits with high spatial resolution in vitro
title_fullStr A MEMS ultrasound stimulation system for modulation of neural circuits with high spatial resolution in vitro
title_full_unstemmed A MEMS ultrasound stimulation system for modulation of neural circuits with high spatial resolution in vitro
title_short A MEMS ultrasound stimulation system for modulation of neural circuits with high spatial resolution in vitro
title_sort mems ultrasound stimulation system for modulation of neural circuits with high spatial resolution in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6799809/
https://www.ncbi.nlm.nih.gov/pubmed/31636922
http://dx.doi.org/10.1038/s41378-019-0070-5
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