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Phenotypic changes of interstitial cells of Cajal after intestinal obstruction in rat model

The objective was to study the effect of mechanical intestinal obstruction in rats on the phenotype of interstitial cells of Cajal (ICC). Healthy Wistar rats were randomly divided into sham-operation group (C), one day obstruction group (M1), two days obstruction group (M2), and three days obstructi...

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Autores principales: Li, Lin, Zou, Changlin, Zhou, Zhenli, Wang, Ximo, Yu, Xiangyang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Associação Brasileira de Divulgação Científica 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6799941/
https://www.ncbi.nlm.nih.gov/pubmed/31618295
http://dx.doi.org/10.1590/1414-431X20198343
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author Li, Lin
Zou, Changlin
Zhou, Zhenli
Wang, Ximo
Yu, Xiangyang
author_facet Li, Lin
Zou, Changlin
Zhou, Zhenli
Wang, Ximo
Yu, Xiangyang
author_sort Li, Lin
collection PubMed
description The objective was to study the effect of mechanical intestinal obstruction in rats on the phenotype of interstitial cells of Cajal (ICC). Healthy Wistar rats were randomly divided into sham-operation group (C), one day obstruction group (M1), two days obstruction group (M2), and three days obstruction group (M3), with 10 rats in each group. The expression of SCF mRNA and c-Kit protein in intestinal tissue was investigated by RT-PCR and immunohistochemistry. Compared with the sham-operation group, the relative expression of SCF mRNA and the expression of c-Kit protein in intestinal tissue were significantly decreased in both obstruction groups. Levels decreased gradually with the prolongation of obstruction time, and significantly decreased on the 3rd day after obstruction (P<0.05). Immunohistochemical staining of the small intestine showed that the number of ICC in the sham-operation group was the highest, and they were gradually decreased with the extension of obstruction time in the M1 to M3 groups. There was a significant difference between groups (P<0.05). Intestinal obstruction caused a decrease in the concentrations of SCF mRNA and c-Kit protein in ICC. With the prolongation of intestinal obstruction, the number of ICCs gradually decreased.
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spelling pubmed-67999412019-10-29 Phenotypic changes of interstitial cells of Cajal after intestinal obstruction in rat model Li, Lin Zou, Changlin Zhou, Zhenli Wang, Ximo Yu, Xiangyang Braz J Med Biol Res Research Article The objective was to study the effect of mechanical intestinal obstruction in rats on the phenotype of interstitial cells of Cajal (ICC). Healthy Wistar rats were randomly divided into sham-operation group (C), one day obstruction group (M1), two days obstruction group (M2), and three days obstruction group (M3), with 10 rats in each group. The expression of SCF mRNA and c-Kit protein in intestinal tissue was investigated by RT-PCR and immunohistochemistry. Compared with the sham-operation group, the relative expression of SCF mRNA and the expression of c-Kit protein in intestinal tissue were significantly decreased in both obstruction groups. Levels decreased gradually with the prolongation of obstruction time, and significantly decreased on the 3rd day after obstruction (P<0.05). Immunohistochemical staining of the small intestine showed that the number of ICC in the sham-operation group was the highest, and they were gradually decreased with the extension of obstruction time in the M1 to M3 groups. There was a significant difference between groups (P<0.05). Intestinal obstruction caused a decrease in the concentrations of SCF mRNA and c-Kit protein in ICC. With the prolongation of intestinal obstruction, the number of ICCs gradually decreased. Associação Brasileira de Divulgação Científica 2019-10-14 /pmc/articles/PMC6799941/ /pubmed/31618295 http://dx.doi.org/10.1590/1414-431X20198343 Text en https://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Lin
Zou, Changlin
Zhou, Zhenli
Wang, Ximo
Yu, Xiangyang
Phenotypic changes of interstitial cells of Cajal after intestinal obstruction in rat model
title Phenotypic changes of interstitial cells of Cajal after intestinal obstruction in rat model
title_full Phenotypic changes of interstitial cells of Cajal after intestinal obstruction in rat model
title_fullStr Phenotypic changes of interstitial cells of Cajal after intestinal obstruction in rat model
title_full_unstemmed Phenotypic changes of interstitial cells of Cajal after intestinal obstruction in rat model
title_short Phenotypic changes of interstitial cells of Cajal after intestinal obstruction in rat model
title_sort phenotypic changes of interstitial cells of cajal after intestinal obstruction in rat model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6799941/
https://www.ncbi.nlm.nih.gov/pubmed/31618295
http://dx.doi.org/10.1590/1414-431X20198343
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