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Lysosomal regulation of extracellular vesicle excretion during D-ribose-induced NLRP3 inflammasome activation in podocytes

The NLRP3 inflammasome is activated in the cytoplasm of cells and its products such as IL-1β are exported through a non-classical ER-Golgi pathway. Several mechanistically distinct models including exocytosis of secretory lysosomes, microvesicles (MVs) and extracellular vehicles (EVs) have been prop...

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Autores principales: Hong, Jinni, Bhat, Owais M., Li, Guangbi, Dempsey, Sara K., Zhang, Qinghua, Ritter, Joseph K., Li, Weiwei, Li, Pin-Lan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6800119/
https://www.ncbi.nlm.nih.gov/pubmed/30771382
http://dx.doi.org/10.1016/j.bbamcr.2019.02.007
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author Hong, Jinni
Bhat, Owais M.
Li, Guangbi
Dempsey, Sara K.
Zhang, Qinghua
Ritter, Joseph K.
Li, Weiwei
Li, Pin-Lan
author_facet Hong, Jinni
Bhat, Owais M.
Li, Guangbi
Dempsey, Sara K.
Zhang, Qinghua
Ritter, Joseph K.
Li, Weiwei
Li, Pin-Lan
author_sort Hong, Jinni
collection PubMed
description The NLRP3 inflammasome is activated in the cytoplasm of cells and its products such as IL-1β are exported through a non-classical ER-Golgi pathway. Several mechanistically distinct models including exocytosis of secretory lysosomes, microvesicles (MVs) and extracellular vehicles (EVs) have been proposed for their release. In this study, we hypothesized that the NLRP3 inflammasome product, IL-1β in response to exogenously administrated and endogenously produced D-ribose stimulation is released via extracellular vesicles including EVs via a sphingolipid-mediated molecular mechanisms controlling lysosome and multivesicular body (MVB) interaction. First, we demonstrated that both endogenous and exogenous D-ribose induced NLRP3 inflammasome activation to produce IL-1β, which was released via EVs in podocytes. Then, we found that colocalization of marker MVB marker VPS16 with IL-1β within podocytes increased upon D-ribose stimulation, which was accompanied by decreased colocalization of lysosome marker Lamp-1 and VPS16, suggesting decrease in MVB inclusion of IL-1β due to reduced lysosome and MVB interaction. All these changes were mimicked and accelerated by lysosome v-ATPase inhibitor, bafilomycin. Moreover, ceramide in podocytes was found elevated upon D-ribose stimulation, and prior treatments of podocyte with acid sphingomyelinase (Asm) inhibitor, amitriptyline, acid ceramidase (AC) inducer, genistein, or AC CRISPR/cas9 activation plasmids were found to decrease D-ribose-induced ceramide accumulation, EVs release and IL-1β secretion due to reduced interactions of lysosome with MVBs. These results suggest that inflammasome-derived products such as IL-1β during D-ribose stimulation are released via EVs, in which lysosomal sphingolipid-mediated regulation of lysosome function plays an important role.
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spelling pubmed-68001192020-05-01 Lysosomal regulation of extracellular vesicle excretion during D-ribose-induced NLRP3 inflammasome activation in podocytes Hong, Jinni Bhat, Owais M. Li, Guangbi Dempsey, Sara K. Zhang, Qinghua Ritter, Joseph K. Li, Weiwei Li, Pin-Lan Biochim Biophys Acta Mol Cell Res Article The NLRP3 inflammasome is activated in the cytoplasm of cells and its products such as IL-1β are exported through a non-classical ER-Golgi pathway. Several mechanistically distinct models including exocytosis of secretory lysosomes, microvesicles (MVs) and extracellular vehicles (EVs) have been proposed for their release. In this study, we hypothesized that the NLRP3 inflammasome product, IL-1β in response to exogenously administrated and endogenously produced D-ribose stimulation is released via extracellular vesicles including EVs via a sphingolipid-mediated molecular mechanisms controlling lysosome and multivesicular body (MVB) interaction. First, we demonstrated that both endogenous and exogenous D-ribose induced NLRP3 inflammasome activation to produce IL-1β, which was released via EVs in podocytes. Then, we found that colocalization of marker MVB marker VPS16 with IL-1β within podocytes increased upon D-ribose stimulation, which was accompanied by decreased colocalization of lysosome marker Lamp-1 and VPS16, suggesting decrease in MVB inclusion of IL-1β due to reduced lysosome and MVB interaction. All these changes were mimicked and accelerated by lysosome v-ATPase inhibitor, bafilomycin. Moreover, ceramide in podocytes was found elevated upon D-ribose stimulation, and prior treatments of podocyte with acid sphingomyelinase (Asm) inhibitor, amitriptyline, acid ceramidase (AC) inducer, genistein, or AC CRISPR/cas9 activation plasmids were found to decrease D-ribose-induced ceramide accumulation, EVs release and IL-1β secretion due to reduced interactions of lysosome with MVBs. These results suggest that inflammasome-derived products such as IL-1β during D-ribose stimulation are released via EVs, in which lysosomal sphingolipid-mediated regulation of lysosome function plays an important role. 2019-02-14 2019-05 /pmc/articles/PMC6800119/ /pubmed/30771382 http://dx.doi.org/10.1016/j.bbamcr.2019.02.007 Text en This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Hong, Jinni
Bhat, Owais M.
Li, Guangbi
Dempsey, Sara K.
Zhang, Qinghua
Ritter, Joseph K.
Li, Weiwei
Li, Pin-Lan
Lysosomal regulation of extracellular vesicle excretion during D-ribose-induced NLRP3 inflammasome activation in podocytes
title Lysosomal regulation of extracellular vesicle excretion during D-ribose-induced NLRP3 inflammasome activation in podocytes
title_full Lysosomal regulation of extracellular vesicle excretion during D-ribose-induced NLRP3 inflammasome activation in podocytes
title_fullStr Lysosomal regulation of extracellular vesicle excretion during D-ribose-induced NLRP3 inflammasome activation in podocytes
title_full_unstemmed Lysosomal regulation of extracellular vesicle excretion during D-ribose-induced NLRP3 inflammasome activation in podocytes
title_short Lysosomal regulation of extracellular vesicle excretion during D-ribose-induced NLRP3 inflammasome activation in podocytes
title_sort lysosomal regulation of extracellular vesicle excretion during d-ribose-induced nlrp3 inflammasome activation in podocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6800119/
https://www.ncbi.nlm.nih.gov/pubmed/30771382
http://dx.doi.org/10.1016/j.bbamcr.2019.02.007
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