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Antitumor Effects of HPV DNA Vaccine Adjuvanted with Beclin-1 as an Autophagy Inducer in a Mice Model

BACKGROUND: There is a growing interest in development of an effective adjuvant system for improving DNA vaccines. Recent findings have confirmed an important role for autophagy in both innate and adaptive immunity. The current study was undertaken to determine the efficacy of autophagy induction wi...

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Autores principales: Naziri, Hamed, Tahamtan, Alireza, Dadmanesh, Maryam, Barati, Mohammad, Ghorban, Khodayar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pasteur Institute of Iran 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6800540/
https://www.ncbi.nlm.nih.gov/pubmed/31104420
http://dx.doi.org/10.29252/ibj.23.6.388
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author Naziri, Hamed
Tahamtan, Alireza
Dadmanesh, Maryam
Barati, Mohammad
Ghorban, Khodayar
author_facet Naziri, Hamed
Tahamtan, Alireza
Dadmanesh, Maryam
Barati, Mohammad
Ghorban, Khodayar
author_sort Naziri, Hamed
collection PubMed
description BACKGROUND: There is a growing interest in development of an effective adjuvant system for improving DNA vaccines. Recent findings have confirmed an important role for autophagy in both innate and adaptive immunity. The current study was undertaken to determine the efficacy of autophagy induction with Beclin-1, as a novel adjuvant system, in mice immunized with human papilloma virus (HPV) DNA vaccine. METHODS: To determine whether autophagy induction with Beclin-1 enhances the efficacy of HPV DNA vaccine, female C57BL/6 mice were challenged with TC-1 tumor cells and were immunized three times at one-week intervals. Two weeks after the final immunization, the mice were sacrificed, and the antitumor effects were assessed by measurement of lymphocyte proliferation, cytotoxicity, cytokine production, and tumor regression. RESULTS: Beclin-1 in combination with HPV-16 DNA vaccine encoding the E7 antigen induced a higher level of lymphocyte proliferation and cytotoxicity than the DNA vaccine alone. The novel combination increased the production of IFN-γ and highly inhibited tumor progression in comparison with DNA vaccine alone. CONCLUSION: Administration of Beclin-1, as an autophagy inducer, with HPV DNA vaccine produces antitumor effects, providing an effective adjuvant for the induction of a strong antitumor immune response.
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spelling pubmed-68005402019-11-01 Antitumor Effects of HPV DNA Vaccine Adjuvanted with Beclin-1 as an Autophagy Inducer in a Mice Model Naziri, Hamed Tahamtan, Alireza Dadmanesh, Maryam Barati, Mohammad Ghorban, Khodayar Iran Biomed J Full Length BACKGROUND: There is a growing interest in development of an effective adjuvant system for improving DNA vaccines. Recent findings have confirmed an important role for autophagy in both innate and adaptive immunity. The current study was undertaken to determine the efficacy of autophagy induction with Beclin-1, as a novel adjuvant system, in mice immunized with human papilloma virus (HPV) DNA vaccine. METHODS: To determine whether autophagy induction with Beclin-1 enhances the efficacy of HPV DNA vaccine, female C57BL/6 mice were challenged with TC-1 tumor cells and were immunized three times at one-week intervals. Two weeks after the final immunization, the mice were sacrificed, and the antitumor effects were assessed by measurement of lymphocyte proliferation, cytotoxicity, cytokine production, and tumor regression. RESULTS: Beclin-1 in combination with HPV-16 DNA vaccine encoding the E7 antigen induced a higher level of lymphocyte proliferation and cytotoxicity than the DNA vaccine alone. The novel combination increased the production of IFN-γ and highly inhibited tumor progression in comparison with DNA vaccine alone. CONCLUSION: Administration of Beclin-1, as an autophagy inducer, with HPV DNA vaccine produces antitumor effects, providing an effective adjuvant for the induction of a strong antitumor immune response. Pasteur Institute of Iran 2019-11 /pmc/articles/PMC6800540/ /pubmed/31104420 http://dx.doi.org/10.29252/ibj.23.6.388 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Full Length
Naziri, Hamed
Tahamtan, Alireza
Dadmanesh, Maryam
Barati, Mohammad
Ghorban, Khodayar
Antitumor Effects of HPV DNA Vaccine Adjuvanted with Beclin-1 as an Autophagy Inducer in a Mice Model
title Antitumor Effects of HPV DNA Vaccine Adjuvanted with Beclin-1 as an Autophagy Inducer in a Mice Model
title_full Antitumor Effects of HPV DNA Vaccine Adjuvanted with Beclin-1 as an Autophagy Inducer in a Mice Model
title_fullStr Antitumor Effects of HPV DNA Vaccine Adjuvanted with Beclin-1 as an Autophagy Inducer in a Mice Model
title_full_unstemmed Antitumor Effects of HPV DNA Vaccine Adjuvanted with Beclin-1 as an Autophagy Inducer in a Mice Model
title_short Antitumor Effects of HPV DNA Vaccine Adjuvanted with Beclin-1 as an Autophagy Inducer in a Mice Model
title_sort antitumor effects of hpv dna vaccine adjuvanted with beclin-1 as an autophagy inducer in a mice model
topic Full Length
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6800540/
https://www.ncbi.nlm.nih.gov/pubmed/31104420
http://dx.doi.org/10.29252/ibj.23.6.388
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