Cargando…

CRISPR/Cas‐based screening of a gene activation library in Saccharomyces cerevisiae identifies a crucial role of OLE1 in thermotolerance

CRISPR/Cas‐based (clustered regularly interspaced short palindromic repeats/CRISPR‐associated) screening has been proved to be an efficient method to study functional genomics from yeast to human. In this study, we report the development of a focused CRISPR/Cas‐based gene activation library in Sacch...

Descripción completa

Detalles Bibliográficos
Autores principales:	Li, Pengsong, Fu, Xiaofen, Zhang, Lei, Li, Shizhong
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2018
Materias:	Research Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6801138/ https://www.ncbi.nlm.nih.gov/pubmed/30394685 http://dx.doi.org/10.1111/1751-7915.13333

Ejemplares similares

The transcription factors Hsf1 and Msn2 of thermotolerant Kluyveromyces marxianus promote cell growth and ethanol fermentation of Saccharomyces cerevisiae at high temperatures
por: Li, Pengsong, et al.
Publicado: (2017)

Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems
por: DiCarlo, James E., et al.
Publicado: (2013)

Tuning CRISPR-Cas9 Gene Drives in Saccharomyces cerevisiae
por: Roggenkamp, Emily, et al.
Publicado: (2018)

An inducible CRISPR interference library for genetic interrogation of Saccharomyces cerevisiae biology
por: Momen-Roknabadi, Amir, et al.
Publicado: (2020)

CRISPR/Cas9 Systems for the Development of Saccharomyces cerevisiae Cell Factories
por: Meng, Jie, et al.
Publicado: (2020)

A website to identify shared genes in Saccharomyces cerevisiae homozygous deletion library screens
por: Temple, Mark D.
Publicado: (2018)

Rapid and Efficient CRISPR/Cas9-Based Mating-Type Switching of Saccharomyces cerevisiae
por: Xie, Ze-Xiong, et al.
Publicado: (2017)

CRISPR-Cas9: A Powerful Tool to Efficiently Engineer Saccharomyces cerevisiae
por: Rainha, João, et al.
Publicado: (2020)

CRISPR/Cas9‐RNA interference system for combinatorial metabolic engineering of Saccharomyces cerevisiae
por: Kildegaard, Kanchana Rueksomtawin, et al.
Publicado: (2019)

Engineering TATA-binding protein Spt15 to improve ethanol tolerance and production in Kluyveromyces marxianus
por: Li, Pengsong, et al.
Publicado: (2018)

CRISPR/Cas9-mediated point mutations improve α-amylase secretion in Saccharomyces cerevisiae
por: Wang, Yanyan, et al.
Publicado: (2022)

Oxygen availability strongly affects chronological lifespan and thermotolerance in batch cultures of Saccharomyces cerevisiae
por: Bisschops, Markus M., et al.
Publicado: (2015)

Seamless site-directed mutagenesis of the Saccharomyces cerevisiae genome using CRISPR-Cas9
por: Biot-Pelletier, Damien, et al.
Publicado: (2016)

CRISPR-Cas genome engineering of esterase activity in Saccharomyces cerevisiae steers aroma formation
por: Dank, Alexander, et al.
Publicado: (2018)

Type I-E CRISPR-Cas System as a Defense System in Saccharomyces cerevisiae
por: Bindal, Gargi, et al.
Publicado: (2022)

Acceleration of CRISPR/Cas9-Mediated Editing at Multiple Sites in the Saccharomyces cerevisiae Genome
por: Karpukhin, Alexey D., et al.
Publicado: (2023)

Identification of a gene, FMP21, whose expression levels are involved in thermotolerance in Saccharomyces cerevisiae
por: Nakamura, Toshihide, et al.
Publicado: (2014)

Bioethanol Production from Cellulose-Rich Corncob Residue by the Thermotolerant Saccharomyces cerevisiae TC-5
por: Boonchuay, Pinpanit, et al.
Publicado: (2021)

A protocol for introduction of multiple genetic modifications in Saccharomyces cerevisiae using CRISPR/Cas9
por: Mans, Robert, et al.
Publicado: (2018)

CRISPR–Cas system enables fast and simple genome editing of industrial Saccharomyces cerevisiae strains
por: Stovicek, Vratislav, et al.
Publicado: (2015)

Proteomic profiling and integrated analysis with transcriptomic data bring new insights in the stress responses of Kluyveromyces marxianus after an arrest during high-temperature ethanol fermentation
por: Li, Pengsong, et al.
Publicado: (2019)

Quantitative and modularized CRISPR/dCas9-dCpf1 dual function system in Saccharomyces cerevisiae
por: Feng, Qing, et al.
Publicado: (2023)

Phenotypic and Genotypic Consequences of CRISPR/Cas9 Editing of the Replication Origins in the rDNA of Saccharomyces cerevisiae
por: Sanchez, Joseph C., et al.
Publicado: (2019)

Saccharomyces cerevisiae Δ9-desaturase Ole1 forms a supercomplex with Slc1 and Dga1
por: Greenwood, Brianna L., et al.
Publicado: (2023)

A Crucial Role of Mitochondrial Dynamics in Dehydration Resistance in Saccharomyces cerevisiae
por: Chen, Chang-Lin, et al.
Publicado: (2021)

CRISPR/Cas-based customization of pooled CRISPR libraries
por: Kweon, Jiyeon, et al.
Publicado: (2018)

Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities
por: Sanson, Kendall R., et al.
Publicado: (2018)

Simple-to-use CRISPR-SpCas9/SaCas9/AsCas12a vector series for genome editing in Saccharomyces cerevisiae
por: Okada, Satoshi, et al.
Publicado: (2021)

CRISPR-Cas9 Approach Constructing Cellulase sestc-Engineered Saccharomyces cerevisiae for the Production of Orange Peel Ethanol
por: Yang, Peizhou, et al.
Publicado: (2018)

Easy efficient HDR-based targeted knock-in in Saccharomyces cerevisiae genome using CRISPR-Cas9 system
por: Singh, Rajveer, et al.
Publicado: (2023)

Overcoming the Limitations of CRISPR-Cas9 Systems in Saccharomyces cerevisiae: Off-Target Effects, Epigenome, and Mitochondrial Editing
por: Sato, Genki, et al.
Publicado: (2023)

Somatic mutations and CRISPR/Cas9 library screening integrated analysis identifies cervical cancer drug‐resistant pathways
por: Cao, Chen, et al.
Publicado: (2021)

Combinatorial library design for improving isobutanol production in Saccharomyces cerevisiae
por: Gambacorta, Francesca V., et al.
Publicado: (2022)

Transcriptional Profiling of Biofilm Regulators Identified by an Overexpression Screen in Saccharomyces cerevisiae
por: Cromie, Gareth A., et al.
Publicado: (2017)

Exploring grape marc as trove for new thermotolerant and inhibitor-tolerant Saccharomyces cerevisiae strains for second-generation bioethanol production
por: Favaro, Lorenzo, et al.
Publicado: (2013)

High Level Ethanol from Sugar Cane Molasses by a New Thermotolerant Saccharomyces cerevisiae Strain in Industrial Scale
por: Fadel, M., et al.
Publicado: (2013)

High-temperature ethanol fermentation from pineapple waste hydrolysate and gene expression analysis of thermotolerant yeast Saccharomyces cerevisiae
por: Phong, Huynh Xuan, et al.
Publicado: (2022)

CRISPR/Cas9: a molecular Swiss army knife for simultaneous introduction of multiple genetic modifications in Saccharomyces cerevisiae
por: Mans, Robert, et al.
Publicado: (2015)

Locus specific engineering of tandem DNA repeats in the genome of Saccharomyces cerevisiae using CRISPR/Cas9 and overlapping oligonucleotides
por: Lancrey, Astrid, et al.
Publicado: (2018)

A toolkit for rapid CRISPR-SpCas9 assisted construction of hexose-transport-deficient Saccharomyces cerevisiae strains
por: Wijsman, Melanie, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_qnpmu86kmdimpmdf698fc89b02