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Expression of multidrug transporter P‐glycoprotein in Pichia pastoris affects the host's methanol metabolism
Pichia pastoris KM71H (Mut(S)) is an efficient producer of hard‐to‐express proteins such as the membrane protein P‐glycoprotein (Pgp), an ATP‐powered efflux pump which is expressed properly, but at very low concentration, using the conventional induction strategy. Evaluation of different induction s...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6801151/ https://www.ncbi.nlm.nih.gov/pubmed/31131547 http://dx.doi.org/10.1111/1751-7915.13420 |