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Expression of multidrug transporter P‐glycoprotein in Pichia pastoris affects the host's methanol metabolism

Pichia pastoris KM71H (Mut(S)) is an efficient producer of hard‐to‐express proteins such as the membrane protein P‐glycoprotein (Pgp), an ATP‐powered efflux pump which is expressed properly, but at very low concentration, using the conventional induction strategy. Evaluation of different induction s...

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Detalles Bibliográficos
Autores principales:	Liu, Wan‐cang, Zhou, Fei, Xia, Di, Shiloach, Joseph
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2019
Materias:	Research Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6801151/ https://www.ncbi.nlm.nih.gov/pubmed/31131547 http://dx.doi.org/10.1111/1751-7915.13420

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