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lncRNA-SNHG15 accelerates the development of hepatocellular carcinoma by targeting miR-490-3p/ histone deacetylase 2 axis

BACKGROUND: Hepatocellular carcinoma (HCC) has become a great threat for people’s health. Many long noncoding RNAs are involved in the pathogenesis of HCC. SNHG15, as a tissue specific long noncoding RNAs, has been studied in many human cancers, except HCC. AIM: To explore the regulatory mechanism o...

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Autores principales: Dai, Wei, Dai, Jia-Liang, Tang, Mao-Hua, Ye, Mu-Shi, Fang, Shuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Baishideng Publishing Group Inc 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6801192/
https://www.ncbi.nlm.nih.gov/pubmed/31636472
http://dx.doi.org/10.3748/wjg.v25.i38.5789
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author Dai, Wei
Dai, Jia-Liang
Tang, Mao-Hua
Ye, Mu-Shi
Fang, Shuo
author_facet Dai, Wei
Dai, Jia-Liang
Tang, Mao-Hua
Ye, Mu-Shi
Fang, Shuo
author_sort Dai, Wei
collection PubMed
description BACKGROUND: Hepatocellular carcinoma (HCC) has become a great threat for people’s health. Many long noncoding RNAs are involved in the pathogenesis of HCC. SNHG15, as a tissue specific long noncoding RNAs, has been studied in many human cancers, except HCC. AIM: To explore the regulatory mechanism of SNHG15 in HCC. METHODS: In the present research, 101 HCC patient samples, two HCC cell lines and one normal liver cell line were used. RT-qPCR and Western blot analysis were applied to detect SNHG15, miR-490-3p and histone deacetylase 2 (HDAC2) expression. The regulatory mechanism of SNHG15 was investigated using CCK-8, Transwell and luciferase reporter assays. RESULTS: Our research showed that up-regulation of SNHG15 was found in HCC and was related to aggressive behaviors in HCC patients. Moreover, knockdown of SNHG15 restrained HCC cell proliferation, migration and invasion. In addition, SNHG15 served as a molecular sponge for miR-490-3p. Further, miR-490-3p directly targets HDAC2. HDAC2 was involved in HCC progression by interacting with the SNHG15/miR-490-3p axis. CONCLUSION: In conclusion, long noncoding RNA SNHG15 promotes HCC progression by mediating the miR-490-3p/HDAC2 axis in HCC.
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spelling pubmed-68011922019-10-21 lncRNA-SNHG15 accelerates the development of hepatocellular carcinoma by targeting miR-490-3p/ histone deacetylase 2 axis Dai, Wei Dai, Jia-Liang Tang, Mao-Hua Ye, Mu-Shi Fang, Shuo World J Gastroenterol Basic Study BACKGROUND: Hepatocellular carcinoma (HCC) has become a great threat for people’s health. Many long noncoding RNAs are involved in the pathogenesis of HCC. SNHG15, as a tissue specific long noncoding RNAs, has been studied in many human cancers, except HCC. AIM: To explore the regulatory mechanism of SNHG15 in HCC. METHODS: In the present research, 101 HCC patient samples, two HCC cell lines and one normal liver cell line were used. RT-qPCR and Western blot analysis were applied to detect SNHG15, miR-490-3p and histone deacetylase 2 (HDAC2) expression. The regulatory mechanism of SNHG15 was investigated using CCK-8, Transwell and luciferase reporter assays. RESULTS: Our research showed that up-regulation of SNHG15 was found in HCC and was related to aggressive behaviors in HCC patients. Moreover, knockdown of SNHG15 restrained HCC cell proliferation, migration and invasion. In addition, SNHG15 served as a molecular sponge for miR-490-3p. Further, miR-490-3p directly targets HDAC2. HDAC2 was involved in HCC progression by interacting with the SNHG15/miR-490-3p axis. CONCLUSION: In conclusion, long noncoding RNA SNHG15 promotes HCC progression by mediating the miR-490-3p/HDAC2 axis in HCC. Baishideng Publishing Group Inc 2019-10-14 2019-10-14 /pmc/articles/PMC6801192/ /pubmed/31636472 http://dx.doi.org/10.3748/wjg.v25.i38.5789 Text en ©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved. http://creativecommons.org/licenses/by-nc/4.0/ This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.
spellingShingle Basic Study
Dai, Wei
Dai, Jia-Liang
Tang, Mao-Hua
Ye, Mu-Shi
Fang, Shuo
lncRNA-SNHG15 accelerates the development of hepatocellular carcinoma by targeting miR-490-3p/ histone deacetylase 2 axis
title lncRNA-SNHG15 accelerates the development of hepatocellular carcinoma by targeting miR-490-3p/ histone deacetylase 2 axis
title_full lncRNA-SNHG15 accelerates the development of hepatocellular carcinoma by targeting miR-490-3p/ histone deacetylase 2 axis
title_fullStr lncRNA-SNHG15 accelerates the development of hepatocellular carcinoma by targeting miR-490-3p/ histone deacetylase 2 axis
title_full_unstemmed lncRNA-SNHG15 accelerates the development of hepatocellular carcinoma by targeting miR-490-3p/ histone deacetylase 2 axis
title_short lncRNA-SNHG15 accelerates the development of hepatocellular carcinoma by targeting miR-490-3p/ histone deacetylase 2 axis
title_sort lncrna-snhg15 accelerates the development of hepatocellular carcinoma by targeting mir-490-3p/ histone deacetylase 2 axis
topic Basic Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6801192/
https://www.ncbi.nlm.nih.gov/pubmed/31636472
http://dx.doi.org/10.3748/wjg.v25.i38.5789
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