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Membrane Topological Model of Glycosyltransferases of the GT-C Superfamily

Glycosyltransferases that use polyisoprenol-linked donor substrates are categorized in the GT-C superfamily. In eukaryotes, they act in the endoplasmic reticulum (ER) lumen and are involved in N-glycosylation, glypiation, O-mannosylation, and C-mannosylation of proteins. We generated a membrane topo...

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Autores principales: Albuquerque-Wendt, Andreia, Hütte, Hermann J., Buettner, Falk F. R., Routier, Françoise H., Bakker, Hans
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6801728/
https://www.ncbi.nlm.nih.gov/pubmed/31569500
http://dx.doi.org/10.3390/ijms20194842
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author Albuquerque-Wendt, Andreia
Hütte, Hermann J.
Buettner, Falk F. R.
Routier, Françoise H.
Bakker, Hans
author_facet Albuquerque-Wendt, Andreia
Hütte, Hermann J.
Buettner, Falk F. R.
Routier, Françoise H.
Bakker, Hans
author_sort Albuquerque-Wendt, Andreia
collection PubMed
description Glycosyltransferases that use polyisoprenol-linked donor substrates are categorized in the GT-C superfamily. In eukaryotes, they act in the endoplasmic reticulum (ER) lumen and are involved in N-glycosylation, glypiation, O-mannosylation, and C-mannosylation of proteins. We generated a membrane topology model of C-mannosyltransferases (DPY19 family) that concurred perfectly with the 13 transmembrane domains (TMDs) observed in oligosaccharyltransferases (STT3 family) structures. A multiple alignment of family members from diverse organisms highlighted the presence of only a few conserved amino acids between DPY19s and STT3s. Most of these residues were shown to be essential for DPY19 function and are positioned in luminal loops that showed high conservation within the DPY19 family. Multiple alignments of other eukaryotic GT-C families underlined the presence of similar conserved motifs in luminal loops, in all enzymes of the superfamily. Most GT-C enzymes are proposed to have an uneven number of TDMs with 11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices. In contrast, PIGM, ALG3, ALG6, and ALG8 have 12 or 14 TMDs and display a C-terminal dilysine ER-retrieval motif oriented towards the cytoplasm. We propose that all members of the GT-C superfamily are evolutionary related enzymes with preserved membrane topology.
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spelling pubmed-68017282019-10-31 Membrane Topological Model of Glycosyltransferases of the GT-C Superfamily Albuquerque-Wendt, Andreia Hütte, Hermann J. Buettner, Falk F. R. Routier, Françoise H. Bakker, Hans Int J Mol Sci Article Glycosyltransferases that use polyisoprenol-linked donor substrates are categorized in the GT-C superfamily. In eukaryotes, they act in the endoplasmic reticulum (ER) lumen and are involved in N-glycosylation, glypiation, O-mannosylation, and C-mannosylation of proteins. We generated a membrane topology model of C-mannosyltransferases (DPY19 family) that concurred perfectly with the 13 transmembrane domains (TMDs) observed in oligosaccharyltransferases (STT3 family) structures. A multiple alignment of family members from diverse organisms highlighted the presence of only a few conserved amino acids between DPY19s and STT3s. Most of these residues were shown to be essential for DPY19 function and are positioned in luminal loops that showed high conservation within the DPY19 family. Multiple alignments of other eukaryotic GT-C families underlined the presence of similar conserved motifs in luminal loops, in all enzymes of the superfamily. Most GT-C enzymes are proposed to have an uneven number of TDMs with 11 (POMT, TMTC, ALG9, ALG12, PIGB, PIGV, and PIGZ) or 13 (DPY19, STT3, and ALG10) membrane-spanning helices. In contrast, PIGM, ALG3, ALG6, and ALG8 have 12 or 14 TMDs and display a C-terminal dilysine ER-retrieval motif oriented towards the cytoplasm. We propose that all members of the GT-C superfamily are evolutionary related enzymes with preserved membrane topology. MDPI 2019-09-29 /pmc/articles/PMC6801728/ /pubmed/31569500 http://dx.doi.org/10.3390/ijms20194842 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Albuquerque-Wendt, Andreia
Hütte, Hermann J.
Buettner, Falk F. R.
Routier, Françoise H.
Bakker, Hans
Membrane Topological Model of Glycosyltransferases of the GT-C Superfamily
title Membrane Topological Model of Glycosyltransferases of the GT-C Superfamily
title_full Membrane Topological Model of Glycosyltransferases of the GT-C Superfamily
title_fullStr Membrane Topological Model of Glycosyltransferases of the GT-C Superfamily
title_full_unstemmed Membrane Topological Model of Glycosyltransferases of the GT-C Superfamily
title_short Membrane Topological Model of Glycosyltransferases of the GT-C Superfamily
title_sort membrane topological model of glycosyltransferases of the gt-c superfamily
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6801728/
https://www.ncbi.nlm.nih.gov/pubmed/31569500
http://dx.doi.org/10.3390/ijms20194842
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