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Membrane Transporters in Human Parotid Gland-Targeted Proteomics Approach
Salivary glands provide secretory functions, including secretion of xenobiotics and among them drugs. However, there is no published information about protein abundance of drug transporters measured using reliable protein quantification methods. Therefore, mRNA expression and absolute protein conten...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6801960/ https://www.ncbi.nlm.nih.gov/pubmed/31569384 http://dx.doi.org/10.3390/ijms20194825 |
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author | Lapczuk-Romanska, Joanna Busch, Diana Gieruszczak, Ewa Drozdzik, Agnieszka Piotrowska, Katarzyna Kowalczyk, Robert Oswald, Stefan Drozdzik, Marek |
author_facet | Lapczuk-Romanska, Joanna Busch, Diana Gieruszczak, Ewa Drozdzik, Agnieszka Piotrowska, Katarzyna Kowalczyk, Robert Oswald, Stefan Drozdzik, Marek |
author_sort | Lapczuk-Romanska, Joanna |
collection | PubMed |
description | Salivary glands provide secretory functions, including secretion of xenobiotics and among them drugs. However, there is no published information about protein abundance of drug transporters measured using reliable protein quantification methods. Therefore, mRNA expression and absolute protein content of clinically relevant ABC (n = 6) and SLC (n = 15) family member transporters in the human parotid gland, using the qRT-PCR and liquid chromatography‒tandem mass spectrometry (LC−MS/MS) method, were studied. The abundance of nearly all measured proteins ranged between 0.04 and 0.45 pmol/mg (OCT3 > MRP1 > PEPT2 > MRP4 > MATE1 > BCRP). mRNAs of ABCB1, ABCC2, ABCC3, SLC10A1, SLC10A2, SLC22A1, SLC22A5, SLC22A6, SLC22A7, SLC22A8, SLCO1A2, SLCO1B1, SLCO1B3 and SLCO2B1 were not detected. The present study provides, for the first time, information about the protein abundance of membrane transporters in the human parotid gland, which could further be used to define salivary bidirectional transport (absorption and secretion) mechanisms of endogenous compounds and xenobiotics. |
format | Online Article Text |
id | pubmed-6801960 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68019602019-10-31 Membrane Transporters in Human Parotid Gland-Targeted Proteomics Approach Lapczuk-Romanska, Joanna Busch, Diana Gieruszczak, Ewa Drozdzik, Agnieszka Piotrowska, Katarzyna Kowalczyk, Robert Oswald, Stefan Drozdzik, Marek Int J Mol Sci Article Salivary glands provide secretory functions, including secretion of xenobiotics and among them drugs. However, there is no published information about protein abundance of drug transporters measured using reliable protein quantification methods. Therefore, mRNA expression and absolute protein content of clinically relevant ABC (n = 6) and SLC (n = 15) family member transporters in the human parotid gland, using the qRT-PCR and liquid chromatography‒tandem mass spectrometry (LC−MS/MS) method, were studied. The abundance of nearly all measured proteins ranged between 0.04 and 0.45 pmol/mg (OCT3 > MRP1 > PEPT2 > MRP4 > MATE1 > BCRP). mRNAs of ABCB1, ABCC2, ABCC3, SLC10A1, SLC10A2, SLC22A1, SLC22A5, SLC22A6, SLC22A7, SLC22A8, SLCO1A2, SLCO1B1, SLCO1B3 and SLCO2B1 were not detected. The present study provides, for the first time, information about the protein abundance of membrane transporters in the human parotid gland, which could further be used to define salivary bidirectional transport (absorption and secretion) mechanisms of endogenous compounds and xenobiotics. MDPI 2019-09-28 /pmc/articles/PMC6801960/ /pubmed/31569384 http://dx.doi.org/10.3390/ijms20194825 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lapczuk-Romanska, Joanna Busch, Diana Gieruszczak, Ewa Drozdzik, Agnieszka Piotrowska, Katarzyna Kowalczyk, Robert Oswald, Stefan Drozdzik, Marek Membrane Transporters in Human Parotid Gland-Targeted Proteomics Approach |
title | Membrane Transporters in Human Parotid Gland-Targeted Proteomics Approach |
title_full | Membrane Transporters in Human Parotid Gland-Targeted Proteomics Approach |
title_fullStr | Membrane Transporters in Human Parotid Gland-Targeted Proteomics Approach |
title_full_unstemmed | Membrane Transporters in Human Parotid Gland-Targeted Proteomics Approach |
title_short | Membrane Transporters in Human Parotid Gland-Targeted Proteomics Approach |
title_sort | membrane transporters in human parotid gland-targeted proteomics approach |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6801960/ https://www.ncbi.nlm.nih.gov/pubmed/31569384 http://dx.doi.org/10.3390/ijms20194825 |
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