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Membrane Transporters in Human Parotid Gland-Targeted Proteomics Approach

Salivary glands provide secretory functions, including secretion of xenobiotics and among them drugs. However, there is no published information about protein abundance of drug transporters measured using reliable protein quantification methods. Therefore, mRNA expression and absolute protein conten...

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Autores principales: Lapczuk-Romanska, Joanna, Busch, Diana, Gieruszczak, Ewa, Drozdzik, Agnieszka, Piotrowska, Katarzyna, Kowalczyk, Robert, Oswald, Stefan, Drozdzik, Marek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6801960/
https://www.ncbi.nlm.nih.gov/pubmed/31569384
http://dx.doi.org/10.3390/ijms20194825
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author Lapczuk-Romanska, Joanna
Busch, Diana
Gieruszczak, Ewa
Drozdzik, Agnieszka
Piotrowska, Katarzyna
Kowalczyk, Robert
Oswald, Stefan
Drozdzik, Marek
author_facet Lapczuk-Romanska, Joanna
Busch, Diana
Gieruszczak, Ewa
Drozdzik, Agnieszka
Piotrowska, Katarzyna
Kowalczyk, Robert
Oswald, Stefan
Drozdzik, Marek
author_sort Lapczuk-Romanska, Joanna
collection PubMed
description Salivary glands provide secretory functions, including secretion of xenobiotics and among them drugs. However, there is no published information about protein abundance of drug transporters measured using reliable protein quantification methods. Therefore, mRNA expression and absolute protein content of clinically relevant ABC (n = 6) and SLC (n = 15) family member transporters in the human parotid gland, using the qRT-PCR and liquid chromatography‒tandem mass spectrometry (LC−MS/MS) method, were studied. The abundance of nearly all measured proteins ranged between 0.04 and 0.45 pmol/mg (OCT3 > MRP1 > PEPT2 > MRP4 > MATE1 > BCRP). mRNAs of ABCB1, ABCC2, ABCC3, SLC10A1, SLC10A2, SLC22A1, SLC22A5, SLC22A6, SLC22A7, SLC22A8, SLCO1A2, SLCO1B1, SLCO1B3 and SLCO2B1 were not detected. The present study provides, for the first time, information about the protein abundance of membrane transporters in the human parotid gland, which could further be used to define salivary bidirectional transport (absorption and secretion) mechanisms of endogenous compounds and xenobiotics.
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spelling pubmed-68019602019-10-31 Membrane Transporters in Human Parotid Gland-Targeted Proteomics Approach Lapczuk-Romanska, Joanna Busch, Diana Gieruszczak, Ewa Drozdzik, Agnieszka Piotrowska, Katarzyna Kowalczyk, Robert Oswald, Stefan Drozdzik, Marek Int J Mol Sci Article Salivary glands provide secretory functions, including secretion of xenobiotics and among them drugs. However, there is no published information about protein abundance of drug transporters measured using reliable protein quantification methods. Therefore, mRNA expression and absolute protein content of clinically relevant ABC (n = 6) and SLC (n = 15) family member transporters in the human parotid gland, using the qRT-PCR and liquid chromatography‒tandem mass spectrometry (LC−MS/MS) method, were studied. The abundance of nearly all measured proteins ranged between 0.04 and 0.45 pmol/mg (OCT3 > MRP1 > PEPT2 > MRP4 > MATE1 > BCRP). mRNAs of ABCB1, ABCC2, ABCC3, SLC10A1, SLC10A2, SLC22A1, SLC22A5, SLC22A6, SLC22A7, SLC22A8, SLCO1A2, SLCO1B1, SLCO1B3 and SLCO2B1 were not detected. The present study provides, for the first time, information about the protein abundance of membrane transporters in the human parotid gland, which could further be used to define salivary bidirectional transport (absorption and secretion) mechanisms of endogenous compounds and xenobiotics. MDPI 2019-09-28 /pmc/articles/PMC6801960/ /pubmed/31569384 http://dx.doi.org/10.3390/ijms20194825 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lapczuk-Romanska, Joanna
Busch, Diana
Gieruszczak, Ewa
Drozdzik, Agnieszka
Piotrowska, Katarzyna
Kowalczyk, Robert
Oswald, Stefan
Drozdzik, Marek
Membrane Transporters in Human Parotid Gland-Targeted Proteomics Approach
title Membrane Transporters in Human Parotid Gland-Targeted Proteomics Approach
title_full Membrane Transporters in Human Parotid Gland-Targeted Proteomics Approach
title_fullStr Membrane Transporters in Human Parotid Gland-Targeted Proteomics Approach
title_full_unstemmed Membrane Transporters in Human Parotid Gland-Targeted Proteomics Approach
title_short Membrane Transporters in Human Parotid Gland-Targeted Proteomics Approach
title_sort membrane transporters in human parotid gland-targeted proteomics approach
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6801960/
https://www.ncbi.nlm.nih.gov/pubmed/31569384
http://dx.doi.org/10.3390/ijms20194825
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