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NET4 Modulates the Compactness of Vacuoles in Arabidopsis thaliana

The dimension of the plants largest organelle—the vacuole—plays a major role in defining cellular elongation rates. The morphology of the vacuole is controlled by the actin cytoskeleton, but molecular players remain largely unknown. Recently, the Networked (NET) family of membrane-associated, actin-...

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Detalles Bibliográficos
Autores principales: Kaiser, Sabrina, Eisa, Ahmed, Kleine-Vehn, Jürgen, Scheuring, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6801981/
https://www.ncbi.nlm.nih.gov/pubmed/31557830
http://dx.doi.org/10.3390/ijms20194752
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author Kaiser, Sabrina
Eisa, Ahmed
Kleine-Vehn, Jürgen
Scheuring, David
author_facet Kaiser, Sabrina
Eisa, Ahmed
Kleine-Vehn, Jürgen
Scheuring, David
author_sort Kaiser, Sabrina
collection PubMed
description The dimension of the plants largest organelle—the vacuole—plays a major role in defining cellular elongation rates. The morphology of the vacuole is controlled by the actin cytoskeleton, but molecular players remain largely unknown. Recently, the Networked (NET) family of membrane-associated, actin-binding proteins has been identified. Here, we show that NET4A localizes to highly constricted regions of the vacuolar membrane and contributes to vacuolar morphology. Using genetic interference, we found that deregulation of NET4 abundance increases vacuolar occupancy, and that overexpression of NET4 abundance decreases vacuolar occupancy. Our data reveal that NET4A induces more compact vacuoles, correlating with reduced cellular and organ growth in Arabidopsis thaliana.
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spelling pubmed-68019812019-10-31 NET4 Modulates the Compactness of Vacuoles in Arabidopsis thaliana Kaiser, Sabrina Eisa, Ahmed Kleine-Vehn, Jürgen Scheuring, David Int J Mol Sci Article The dimension of the plants largest organelle—the vacuole—plays a major role in defining cellular elongation rates. The morphology of the vacuole is controlled by the actin cytoskeleton, but molecular players remain largely unknown. Recently, the Networked (NET) family of membrane-associated, actin-binding proteins has been identified. Here, we show that NET4A localizes to highly constricted regions of the vacuolar membrane and contributes to vacuolar morphology. Using genetic interference, we found that deregulation of NET4 abundance increases vacuolar occupancy, and that overexpression of NET4 abundance decreases vacuolar occupancy. Our data reveal that NET4A induces more compact vacuoles, correlating with reduced cellular and organ growth in Arabidopsis thaliana. MDPI 2019-09-25 /pmc/articles/PMC6801981/ /pubmed/31557830 http://dx.doi.org/10.3390/ijms20194752 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kaiser, Sabrina
Eisa, Ahmed
Kleine-Vehn, Jürgen
Scheuring, David
NET4 Modulates the Compactness of Vacuoles in Arabidopsis thaliana
title NET4 Modulates the Compactness of Vacuoles in Arabidopsis thaliana
title_full NET4 Modulates the Compactness of Vacuoles in Arabidopsis thaliana
title_fullStr NET4 Modulates the Compactness of Vacuoles in Arabidopsis thaliana
title_full_unstemmed NET4 Modulates the Compactness of Vacuoles in Arabidopsis thaliana
title_short NET4 Modulates the Compactness of Vacuoles in Arabidopsis thaliana
title_sort net4 modulates the compactness of vacuoles in arabidopsis thaliana
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6801981/
https://www.ncbi.nlm.nih.gov/pubmed/31557830
http://dx.doi.org/10.3390/ijms20194752
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