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NET4 Modulates the Compactness of Vacuoles in Arabidopsis thaliana
The dimension of the plants largest organelle—the vacuole—plays a major role in defining cellular elongation rates. The morphology of the vacuole is controlled by the actin cytoskeleton, but molecular players remain largely unknown. Recently, the Networked (NET) family of membrane-associated, actin-...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6801981/ https://www.ncbi.nlm.nih.gov/pubmed/31557830 http://dx.doi.org/10.3390/ijms20194752 |
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author | Kaiser, Sabrina Eisa, Ahmed Kleine-Vehn, Jürgen Scheuring, David |
author_facet | Kaiser, Sabrina Eisa, Ahmed Kleine-Vehn, Jürgen Scheuring, David |
author_sort | Kaiser, Sabrina |
collection | PubMed |
description | The dimension of the plants largest organelle—the vacuole—plays a major role in defining cellular elongation rates. The morphology of the vacuole is controlled by the actin cytoskeleton, but molecular players remain largely unknown. Recently, the Networked (NET) family of membrane-associated, actin-binding proteins has been identified. Here, we show that NET4A localizes to highly constricted regions of the vacuolar membrane and contributes to vacuolar morphology. Using genetic interference, we found that deregulation of NET4 abundance increases vacuolar occupancy, and that overexpression of NET4 abundance decreases vacuolar occupancy. Our data reveal that NET4A induces more compact vacuoles, correlating with reduced cellular and organ growth in Arabidopsis thaliana. |
format | Online Article Text |
id | pubmed-6801981 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68019812019-10-31 NET4 Modulates the Compactness of Vacuoles in Arabidopsis thaliana Kaiser, Sabrina Eisa, Ahmed Kleine-Vehn, Jürgen Scheuring, David Int J Mol Sci Article The dimension of the plants largest organelle—the vacuole—plays a major role in defining cellular elongation rates. The morphology of the vacuole is controlled by the actin cytoskeleton, but molecular players remain largely unknown. Recently, the Networked (NET) family of membrane-associated, actin-binding proteins has been identified. Here, we show that NET4A localizes to highly constricted regions of the vacuolar membrane and contributes to vacuolar morphology. Using genetic interference, we found that deregulation of NET4 abundance increases vacuolar occupancy, and that overexpression of NET4 abundance decreases vacuolar occupancy. Our data reveal that NET4A induces more compact vacuoles, correlating with reduced cellular and organ growth in Arabidopsis thaliana. MDPI 2019-09-25 /pmc/articles/PMC6801981/ /pubmed/31557830 http://dx.doi.org/10.3390/ijms20194752 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kaiser, Sabrina Eisa, Ahmed Kleine-Vehn, Jürgen Scheuring, David NET4 Modulates the Compactness of Vacuoles in Arabidopsis thaliana |
title | NET4 Modulates the Compactness of Vacuoles in Arabidopsis thaliana |
title_full | NET4 Modulates the Compactness of Vacuoles in Arabidopsis thaliana |
title_fullStr | NET4 Modulates the Compactness of Vacuoles in Arabidopsis thaliana |
title_full_unstemmed | NET4 Modulates the Compactness of Vacuoles in Arabidopsis thaliana |
title_short | NET4 Modulates the Compactness of Vacuoles in Arabidopsis thaliana |
title_sort | net4 modulates the compactness of vacuoles in arabidopsis thaliana |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6801981/ https://www.ncbi.nlm.nih.gov/pubmed/31557830 http://dx.doi.org/10.3390/ijms20194752 |
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