The wild-type flagellar filament of the Firmicute Kurthia at 2.8 Å resolution in vivo

Bacteria swim and swarm by rotating the micrometers long, helical filaments of their flagella. They change direction by reversing their flagellar rotation, which switches the handedness of the filament’s supercoil. So far, all studied functional filaments are composed of a mixture of L- and R-state...

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Autores principales: Blum, Thorsten B., Filippidou, Sevasti, Fatton, Mathilda, Junier, Pilar, Abrahams, Jan Pieter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6802091/
https://www.ncbi.nlm.nih.gov/pubmed/31628388
http://dx.doi.org/10.1038/s41598-019-51440-1
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author Blum, Thorsten B.
Filippidou, Sevasti
Fatton, Mathilda
Junier, Pilar
Abrahams, Jan Pieter
author_facet Blum, Thorsten B.
Filippidou, Sevasti
Fatton, Mathilda
Junier, Pilar
Abrahams, Jan Pieter
author_sort Blum, Thorsten B.
collection PubMed
description Bacteria swim and swarm by rotating the micrometers long, helical filaments of their flagella. They change direction by reversing their flagellar rotation, which switches the handedness of the filament’s supercoil. So far, all studied functional filaments are composed of a mixture of L- and R-state flagellin monomers. Here we show in a study of the wild type Firmicute Kurthia sp., that curved, functional filaments can adopt a conformation in vivo that is closely related to a uniform, all-L-state. This sheds additional light on transitions of the flagellar supercoil and uniquely reveals the atomic structure of a wild-type flagellar filament in vivo, including six residues showing clearly densities of O-linked glycosylation.
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spelling pubmed-68020912019-10-24 The wild-type flagellar filament of the Firmicute Kurthia at 2.8 Å resolution in vivo Blum, Thorsten B. Filippidou, Sevasti Fatton, Mathilda Junier, Pilar Abrahams, Jan Pieter Sci Rep Article Bacteria swim and swarm by rotating the micrometers long, helical filaments of their flagella. They change direction by reversing their flagellar rotation, which switches the handedness of the filament’s supercoil. So far, all studied functional filaments are composed of a mixture of L- and R-state flagellin monomers. Here we show in a study of the wild type Firmicute Kurthia sp., that curved, functional filaments can adopt a conformation in vivo that is closely related to a uniform, all-L-state. This sheds additional light on transitions of the flagellar supercoil and uniquely reveals the atomic structure of a wild-type flagellar filament in vivo, including six residues showing clearly densities of O-linked glycosylation. Nature Publishing Group UK 2019-10-18 /pmc/articles/PMC6802091/ /pubmed/31628388 http://dx.doi.org/10.1038/s41598-019-51440-1 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Blum, Thorsten B.
Filippidou, Sevasti
Fatton, Mathilda
Junier, Pilar
Abrahams, Jan Pieter
The wild-type flagellar filament of the Firmicute Kurthia at 2.8 Å resolution in vivo
title The wild-type flagellar filament of the Firmicute Kurthia at 2.8 Å resolution in vivo
title_full The wild-type flagellar filament of the Firmicute Kurthia at 2.8 Å resolution in vivo
title_fullStr The wild-type flagellar filament of the Firmicute Kurthia at 2.8 Å resolution in vivo
title_full_unstemmed The wild-type flagellar filament of the Firmicute Kurthia at 2.8 Å resolution in vivo
title_short The wild-type flagellar filament of the Firmicute Kurthia at 2.8 Å resolution in vivo
title_sort wild-type flagellar filament of the firmicute kurthia at 2.8 å resolution in vivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6802091/
https://www.ncbi.nlm.nih.gov/pubmed/31628388
http://dx.doi.org/10.1038/s41598-019-51440-1
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