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Optical mapping of biological water in single live cells by stimulated Raman excited fluorescence microscopy

Water is arguably the most common and yet least understood material on Earth. Indeed, the biophysical behavior of water in crowded intracellular milieu is a long-debated issue. Understanding of the spatial and compositional heterogeneity of water inside cells remains elusive, largely due to a lack o...

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Detalles Bibliográficos
Autores principales: Shi, Lixue, Hu, Fanghao, Min, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6802100/
https://www.ncbi.nlm.nih.gov/pubmed/31628307
http://dx.doi.org/10.1038/s41467-019-12708-2
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author Shi, Lixue
Hu, Fanghao
Min, Wei
author_facet Shi, Lixue
Hu, Fanghao
Min, Wei
author_sort Shi, Lixue
collection PubMed
description Water is arguably the most common and yet least understood material on Earth. Indeed, the biophysical behavior of water in crowded intracellular milieu is a long-debated issue. Understanding of the spatial and compositional heterogeneity of water inside cells remains elusive, largely due to a lack of proper water-sensing tools with high sensitivity and spatial resolution. Recently, stimulated Raman excited fluorescence (SREF) microscopy was reported as the most sensitive vibrational imaging in the optical far field. Herein we develop SREF into a water-sensing tool by coupling it with vibrational solvatochromism. This technique allows us to directly visualize spatially-resolved distribution of water states inside single mammalian cells. Qualitatively, our result supports the concept of biological water and reveals intracellular water heterogeneity between nucleus and cytoplasm. Quantitatively, we unveil a compositional map of the water pool inside living cells. Hence we hope SREF will be a promising tool to study intracellular water and its relationship with cellular activities.
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spelling pubmed-68021002019-10-22 Optical mapping of biological water in single live cells by stimulated Raman excited fluorescence microscopy Shi, Lixue Hu, Fanghao Min, Wei Nat Commun Article Water is arguably the most common and yet least understood material on Earth. Indeed, the biophysical behavior of water in crowded intracellular milieu is a long-debated issue. Understanding of the spatial and compositional heterogeneity of water inside cells remains elusive, largely due to a lack of proper water-sensing tools with high sensitivity and spatial resolution. Recently, stimulated Raman excited fluorescence (SREF) microscopy was reported as the most sensitive vibrational imaging in the optical far field. Herein we develop SREF into a water-sensing tool by coupling it with vibrational solvatochromism. This technique allows us to directly visualize spatially-resolved distribution of water states inside single mammalian cells. Qualitatively, our result supports the concept of biological water and reveals intracellular water heterogeneity between nucleus and cytoplasm. Quantitatively, we unveil a compositional map of the water pool inside living cells. Hence we hope SREF will be a promising tool to study intracellular water and its relationship with cellular activities. Nature Publishing Group UK 2019-10-18 /pmc/articles/PMC6802100/ /pubmed/31628307 http://dx.doi.org/10.1038/s41467-019-12708-2 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Shi, Lixue
Hu, Fanghao
Min, Wei
Optical mapping of biological water in single live cells by stimulated Raman excited fluorescence microscopy
title Optical mapping of biological water in single live cells by stimulated Raman excited fluorescence microscopy
title_full Optical mapping of biological water in single live cells by stimulated Raman excited fluorescence microscopy
title_fullStr Optical mapping of biological water in single live cells by stimulated Raman excited fluorescence microscopy
title_full_unstemmed Optical mapping of biological water in single live cells by stimulated Raman excited fluorescence microscopy
title_short Optical mapping of biological water in single live cells by stimulated Raman excited fluorescence microscopy
title_sort optical mapping of biological water in single live cells by stimulated raman excited fluorescence microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6802100/
https://www.ncbi.nlm.nih.gov/pubmed/31628307
http://dx.doi.org/10.1038/s41467-019-12708-2
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