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Optical mapping of biological water in single live cells by stimulated Raman excited fluorescence microscopy
Water is arguably the most common and yet least understood material on Earth. Indeed, the biophysical behavior of water in crowded intracellular milieu is a long-debated issue. Understanding of the spatial and compositional heterogeneity of water inside cells remains elusive, largely due to a lack o...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6802100/ https://www.ncbi.nlm.nih.gov/pubmed/31628307 http://dx.doi.org/10.1038/s41467-019-12708-2 |
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author | Shi, Lixue Hu, Fanghao Min, Wei |
author_facet | Shi, Lixue Hu, Fanghao Min, Wei |
author_sort | Shi, Lixue |
collection | PubMed |
description | Water is arguably the most common and yet least understood material on Earth. Indeed, the biophysical behavior of water in crowded intracellular milieu is a long-debated issue. Understanding of the spatial and compositional heterogeneity of water inside cells remains elusive, largely due to a lack of proper water-sensing tools with high sensitivity and spatial resolution. Recently, stimulated Raman excited fluorescence (SREF) microscopy was reported as the most sensitive vibrational imaging in the optical far field. Herein we develop SREF into a water-sensing tool by coupling it with vibrational solvatochromism. This technique allows us to directly visualize spatially-resolved distribution of water states inside single mammalian cells. Qualitatively, our result supports the concept of biological water and reveals intracellular water heterogeneity between nucleus and cytoplasm. Quantitatively, we unveil a compositional map of the water pool inside living cells. Hence we hope SREF will be a promising tool to study intracellular water and its relationship with cellular activities. |
format | Online Article Text |
id | pubmed-6802100 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-68021002019-10-22 Optical mapping of biological water in single live cells by stimulated Raman excited fluorescence microscopy Shi, Lixue Hu, Fanghao Min, Wei Nat Commun Article Water is arguably the most common and yet least understood material on Earth. Indeed, the biophysical behavior of water in crowded intracellular milieu is a long-debated issue. Understanding of the spatial and compositional heterogeneity of water inside cells remains elusive, largely due to a lack of proper water-sensing tools with high sensitivity and spatial resolution. Recently, stimulated Raman excited fluorescence (SREF) microscopy was reported as the most sensitive vibrational imaging in the optical far field. Herein we develop SREF into a water-sensing tool by coupling it with vibrational solvatochromism. This technique allows us to directly visualize spatially-resolved distribution of water states inside single mammalian cells. Qualitatively, our result supports the concept of biological water and reveals intracellular water heterogeneity between nucleus and cytoplasm. Quantitatively, we unveil a compositional map of the water pool inside living cells. Hence we hope SREF will be a promising tool to study intracellular water and its relationship with cellular activities. Nature Publishing Group UK 2019-10-18 /pmc/articles/PMC6802100/ /pubmed/31628307 http://dx.doi.org/10.1038/s41467-019-12708-2 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Shi, Lixue Hu, Fanghao Min, Wei Optical mapping of biological water in single live cells by stimulated Raman excited fluorescence microscopy |
title | Optical mapping of biological water in single live cells by stimulated Raman excited fluorescence microscopy |
title_full | Optical mapping of biological water in single live cells by stimulated Raman excited fluorescence microscopy |
title_fullStr | Optical mapping of biological water in single live cells by stimulated Raman excited fluorescence microscopy |
title_full_unstemmed | Optical mapping of biological water in single live cells by stimulated Raman excited fluorescence microscopy |
title_short | Optical mapping of biological water in single live cells by stimulated Raman excited fluorescence microscopy |
title_sort | optical mapping of biological water in single live cells by stimulated raman excited fluorescence microscopy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6802100/ https://www.ncbi.nlm.nih.gov/pubmed/31628307 http://dx.doi.org/10.1038/s41467-019-12708-2 |
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