DNA extraction for human microbiome studies: the issue of standardization

Among the laboratory and bioinformatic processing steps for human microbiome studies, a lack of consistency in DNA extraction methodologies is hindering the ability to compare results between studies and sometimes leading to errant conclusions. The purpose of this article is to highlight the issues...

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Detalles Bibliográficos
Autores principales: Greathouse, K. Leigh, Sinha, Rashmi, Vogtmann, Emily
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6802309/
https://www.ncbi.nlm.nih.gov/pubmed/31639026
http://dx.doi.org/10.1186/s13059-019-1843-8
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author Greathouse, K. Leigh
Sinha, Rashmi
Vogtmann, Emily
author_facet Greathouse, K. Leigh
Sinha, Rashmi
Vogtmann, Emily
author_sort Greathouse, K. Leigh
collection PubMed
description Among the laboratory and bioinformatic processing steps for human microbiome studies, a lack of consistency in DNA extraction methodologies is hindering the ability to compare results between studies and sometimes leading to errant conclusions. The purpose of this article is to highlight the issues related to DNA extraction methods and to suggest minimum standard requirements that should be followed to ensure consistency and reproducibility.
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spelling pubmed-68023092019-10-22 DNA extraction for human microbiome studies: the issue of standardization Greathouse, K. Leigh Sinha, Rashmi Vogtmann, Emily Genome Biol Review Among the laboratory and bioinformatic processing steps for human microbiome studies, a lack of consistency in DNA extraction methodologies is hindering the ability to compare results between studies and sometimes leading to errant conclusions. The purpose of this article is to highlight the issues related to DNA extraction methods and to suggest minimum standard requirements that should be followed to ensure consistency and reproducibility. BioMed Central 2019-10-21 /pmc/articles/PMC6802309/ /pubmed/31639026 http://dx.doi.org/10.1186/s13059-019-1843-8 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Review
Greathouse, K. Leigh
Sinha, Rashmi
Vogtmann, Emily
DNA extraction for human microbiome studies: the issue of standardization
title DNA extraction for human microbiome studies: the issue of standardization
title_full DNA extraction for human microbiome studies: the issue of standardization
title_fullStr DNA extraction for human microbiome studies: the issue of standardization
title_full_unstemmed DNA extraction for human microbiome studies: the issue of standardization
title_short DNA extraction for human microbiome studies: the issue of standardization
title_sort dna extraction for human microbiome studies: the issue of standardization
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6802309/
https://www.ncbi.nlm.nih.gov/pubmed/31639026
http://dx.doi.org/10.1186/s13059-019-1843-8
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