Designing and optimization of an electrochemical substitute for the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assay

For the first time ever, this paper reports the development of an easily operated and cost-effective electrochemical assay to be used as an appropriate substitute for the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assay. The proposed assay is based on the elect...

Descripción completa

Detalles Bibliográficos
Autores principales: Mazloum-Ardakani, Mohammad, Barazesh, Behnaz, Moshtaghioun, Seyed Mohammad, Sheikhha, Mohammad Hasan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6802382/
https://www.ncbi.nlm.nih.gov/pubmed/31628354
http://dx.doi.org/10.1038/s41598-019-51241-6
_version_ 1783460789307310080
author Mazloum-Ardakani, Mohammad
Barazesh, Behnaz
Moshtaghioun, Seyed Mohammad
Sheikhha, Mohammad Hasan
author_facet Mazloum-Ardakani, Mohammad
Barazesh, Behnaz
Moshtaghioun, Seyed Mohammad
Sheikhha, Mohammad Hasan
author_sort Mazloum-Ardakani, Mohammad
collection PubMed
description For the first time ever, this paper reports the development of an easily operated and cost-effective electrochemical assay to be used as an appropriate substitute for the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assay. The proposed assay is based on the electrochemical reaction of Saccharomyces cerevisiae (S. cerevisiae) with toxic materials, and it overcomes most of the limitations of MTT such as evaporation of volatile solvents, cytotoxic effects of MTT reagents, high cost, and sensitivity to light. The novel electrochemical assay can be used to detect diazinon in the range of 10(−6) g mL(−1) to 10(−2) g mL(−1) with the detection limit of 1.5 × 10(−7) g mL(−1).
format Online
Article
Text
id pubmed-6802382
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-68023822019-10-24 Designing and optimization of an electrochemical substitute for the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assay Mazloum-Ardakani, Mohammad Barazesh, Behnaz Moshtaghioun, Seyed Mohammad Sheikhha, Mohammad Hasan Sci Rep Article For the first time ever, this paper reports the development of an easily operated and cost-effective electrochemical assay to be used as an appropriate substitute for the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assay. The proposed assay is based on the electrochemical reaction of Saccharomyces cerevisiae (S. cerevisiae) with toxic materials, and it overcomes most of the limitations of MTT such as evaporation of volatile solvents, cytotoxic effects of MTT reagents, high cost, and sensitivity to light. The novel electrochemical assay can be used to detect diazinon in the range of 10(−6) g mL(−1) to 10(−2) g mL(−1) with the detection limit of 1.5 × 10(−7) g mL(−1). Nature Publishing Group UK 2019-10-18 /pmc/articles/PMC6802382/ /pubmed/31628354 http://dx.doi.org/10.1038/s41598-019-51241-6 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Mazloum-Ardakani, Mohammad
Barazesh, Behnaz
Moshtaghioun, Seyed Mohammad
Sheikhha, Mohammad Hasan
Designing and optimization of an electrochemical substitute for the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assay
title Designing and optimization of an electrochemical substitute for the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assay
title_full Designing and optimization of an electrochemical substitute for the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assay
title_fullStr Designing and optimization of an electrochemical substitute for the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assay
title_full_unstemmed Designing and optimization of an electrochemical substitute for the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assay
title_short Designing and optimization of an electrochemical substitute for the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assay
title_sort designing and optimization of an electrochemical substitute for the mtt (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assay
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6802382/
https://www.ncbi.nlm.nih.gov/pubmed/31628354
http://dx.doi.org/10.1038/s41598-019-51241-6
work_keys_str_mv AT mazloumardakanimohammad designingandoptimizationofanelectrochemicalsubstituteforthemtt345dimethylthiazol2yl25diphenyltetrazoliumbromidecellviabilityassay
AT barazeshbehnaz designingandoptimizationofanelectrochemicalsubstituteforthemtt345dimethylthiazol2yl25diphenyltetrazoliumbromidecellviabilityassay
AT moshtaghiounseyedmohammad designingandoptimizationofanelectrochemicalsubstituteforthemtt345dimethylthiazol2yl25diphenyltetrazoliumbromidecellviabilityassay
AT sheikhhamohammadhasan designingandoptimizationofanelectrochemicalsubstituteforthemtt345dimethylthiazol2yl25diphenyltetrazoliumbromidecellviabilityassay

Ejemplares similares