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Molecular epidemiology of Mycoplasma hyorhinis porcine field isolates in the United States
Mycoplasma hyorhinis is one of the causative agents of polyserositis and arthritis in post-weaning pigs. Here we describe the development of a multi-locus sequence typing (MLST) protocol for the characterization of M. hyorhinis field isolates. A total of 104 field isolates from different geographica...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6802821/ https://www.ncbi.nlm.nih.gov/pubmed/31634349 http://dx.doi.org/10.1371/journal.pone.0223653 |
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author | Clavijo, Maria J. Sreevatsan, Srinand Johnson, Timothy J. Rovira, Albert |
author_facet | Clavijo, Maria J. Sreevatsan, Srinand Johnson, Timothy J. Rovira, Albert |
author_sort | Clavijo, Maria J. |
collection | PubMed |
description | Mycoplasma hyorhinis is one of the causative agents of polyserositis and arthritis in post-weaning pigs. Here we describe the development of a multi-locus sequence typing (MLST) protocol for the characterization of M. hyorhinis field isolates. A total of 104 field isolates from different geographical locations, swine production systems, and clinical backgrounds, were analyzed. Twenty-seven genes, including housekeeping and those encoding surface proteins, were evaluated to index diversity. Genes encoding surface proteins were included to increase the discriminatory power of the MLST. Four target gene fragments were selected to be included in the final MLST-s (surface) protocol: pdhB, p95, mtlD and ung. Within each locus the nucleotide variation ranged from 1.4% to 20%. The 104 field isolates were classified into 39 distinct sequence types (STs). Multiple STs were found within the same production system and within the same pig. The majority of STs grouped strains from the same production system; however, cases existed where multiple systems shared a ST, indicating potential relationships between pig flows. The majority of the nucleotide changes observed in these genes generated synonymous changes, while non-synonymous changes were exclusively in the mtlD gene fragment, suggesting that this protein is undergoing selection. Molecular typing of M. hyorhinis will primarily aid swine practitioners with pig flow management and identifying sources of infection during outbreaks. |
format | Online Article Text |
id | pubmed-6802821 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-68028212019-11-02 Molecular epidemiology of Mycoplasma hyorhinis porcine field isolates in the United States Clavijo, Maria J. Sreevatsan, Srinand Johnson, Timothy J. Rovira, Albert PLoS One Research Article Mycoplasma hyorhinis is one of the causative agents of polyserositis and arthritis in post-weaning pigs. Here we describe the development of a multi-locus sequence typing (MLST) protocol for the characterization of M. hyorhinis field isolates. A total of 104 field isolates from different geographical locations, swine production systems, and clinical backgrounds, were analyzed. Twenty-seven genes, including housekeeping and those encoding surface proteins, were evaluated to index diversity. Genes encoding surface proteins were included to increase the discriminatory power of the MLST. Four target gene fragments were selected to be included in the final MLST-s (surface) protocol: pdhB, p95, mtlD and ung. Within each locus the nucleotide variation ranged from 1.4% to 20%. The 104 field isolates were classified into 39 distinct sequence types (STs). Multiple STs were found within the same production system and within the same pig. The majority of STs grouped strains from the same production system; however, cases existed where multiple systems shared a ST, indicating potential relationships between pig flows. The majority of the nucleotide changes observed in these genes generated synonymous changes, while non-synonymous changes were exclusively in the mtlD gene fragment, suggesting that this protein is undergoing selection. Molecular typing of M. hyorhinis will primarily aid swine practitioners with pig flow management and identifying sources of infection during outbreaks. Public Library of Science 2019-10-21 /pmc/articles/PMC6802821/ /pubmed/31634349 http://dx.doi.org/10.1371/journal.pone.0223653 Text en © 2019 Clavijo et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Clavijo, Maria J. Sreevatsan, Srinand Johnson, Timothy J. Rovira, Albert Molecular epidemiology of Mycoplasma hyorhinis porcine field isolates in the United States |
title | Molecular epidemiology of Mycoplasma hyorhinis porcine field isolates in the United States |
title_full | Molecular epidemiology of Mycoplasma hyorhinis porcine field isolates in the United States |
title_fullStr | Molecular epidemiology of Mycoplasma hyorhinis porcine field isolates in the United States |
title_full_unstemmed | Molecular epidemiology of Mycoplasma hyorhinis porcine field isolates in the United States |
title_short | Molecular epidemiology of Mycoplasma hyorhinis porcine field isolates in the United States |
title_sort | molecular epidemiology of mycoplasma hyorhinis porcine field isolates in the united states |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6802821/ https://www.ncbi.nlm.nih.gov/pubmed/31634349 http://dx.doi.org/10.1371/journal.pone.0223653 |
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