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Chromosome spreading of the (TTAGGG)n repeats in the Pipa carvalhoi Miranda-Ribeiro, 1937 (Pipidae, Anura) karyotype

Abstract. Pipidae is a clade of Anura that diverged relatively early from other frogs in the phylogeny of the group. Pipids have a unique combination of morphological features, some of which appear to represent a mix of adaptations to aquatic life and plesiomorphic characters of Anura. The present s...

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Autores principales: Zattera, Michelle Louise, Lima, Luana, Duarte, Iraine, de Sousa, Deborah Yasmin, Araújo, Olívia Gabriela dos Santos, Gazoni, Thiago, Mott, Tamí, Recco-Pimentel, Shirlei Maria, Bruschi, Daniel Pacheco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pensoft Publishers 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6803351/
https://www.ncbi.nlm.nih.gov/pubmed/31649799
http://dx.doi.org/10.3897/CompCytogen.v13i3.35524
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author Zattera, Michelle Louise
Lima, Luana
Duarte, Iraine
de Sousa, Deborah Yasmin
Araújo, Olívia Gabriela dos Santos
Gazoni, Thiago
Mott, Tamí
Recco-Pimentel, Shirlei Maria
Bruschi, Daniel Pacheco
author_facet Zattera, Michelle Louise
Lima, Luana
Duarte, Iraine
de Sousa, Deborah Yasmin
Araújo, Olívia Gabriela dos Santos
Gazoni, Thiago
Mott, Tamí
Recco-Pimentel, Shirlei Maria
Bruschi, Daniel Pacheco
author_sort Zattera, Michelle Louise
collection PubMed
description Abstract. Pipidae is a clade of Anura that diverged relatively early from other frogs in the phylogeny of the group. Pipids have a unique combination of morphological features, some of which appear to represent a mix of adaptations to aquatic life and plesiomorphic characters of Anura. The present study describes the karyotype of Pipa carvalhoi Miranda-Ribeiro, 1937, including morphology, heterochromatin distribution, and location of the NOR site. The diploid number of P. carvalhoi is 2n=20, including three metacentric pairs (1, 4, 8), two submetacentric (2 and 7), three subtelocentric (3, 5, 6), and two telocentric pairs (9 and 10). C-banding detected centromeric blocks of heterochromatin in all chromosome pairs and the NOR detected in chromosome pair 9, as confirmed by FISH using the rDNA 28S probe. The telomeric probes indicated the presence of interstitial telomeric sequences (ITSs), primarily in the centromeric region of the chromosomes, frequently associated with heterochromatin, suggesting that these repeats are a significant component of this region. The findings of the present study provide important insights for the understanding of the mechanisms of chromosomal evolution in the genus Pipa, and the diversification of the Pipidae as a whole.
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spelling pubmed-68033512019-10-24 Chromosome spreading of the (TTAGGG)n repeats in the Pipa carvalhoi Miranda-Ribeiro, 1937 (Pipidae, Anura) karyotype Zattera, Michelle Louise Lima, Luana Duarte, Iraine de Sousa, Deborah Yasmin Araújo, Olívia Gabriela dos Santos Gazoni, Thiago Mott, Tamí Recco-Pimentel, Shirlei Maria Bruschi, Daniel Pacheco Comp Cytogenet Research Article Abstract. Pipidae is a clade of Anura that diverged relatively early from other frogs in the phylogeny of the group. Pipids have a unique combination of morphological features, some of which appear to represent a mix of adaptations to aquatic life and plesiomorphic characters of Anura. The present study describes the karyotype of Pipa carvalhoi Miranda-Ribeiro, 1937, including morphology, heterochromatin distribution, and location of the NOR site. The diploid number of P. carvalhoi is 2n=20, including three metacentric pairs (1, 4, 8), two submetacentric (2 and 7), three subtelocentric (3, 5, 6), and two telocentric pairs (9 and 10). C-banding detected centromeric blocks of heterochromatin in all chromosome pairs and the NOR detected in chromosome pair 9, as confirmed by FISH using the rDNA 28S probe. The telomeric probes indicated the presence of interstitial telomeric sequences (ITSs), primarily in the centromeric region of the chromosomes, frequently associated with heterochromatin, suggesting that these repeats are a significant component of this region. The findings of the present study provide important insights for the understanding of the mechanisms of chromosomal evolution in the genus Pipa, and the diversification of the Pipidae as a whole. Pensoft Publishers 2019-10-14 /pmc/articles/PMC6803351/ /pubmed/31649799 http://dx.doi.org/10.3897/CompCytogen.v13i3.35524 Text en Michelle Louise Zattera, Luana Lima, Iraine Duarte, Deborah Yasmin de Sousa, Olívia Gabriela dos Santos Araújo, Thiago Gazoni, Tamí Mott, Shirlei Maria Recco-Pimentel, Daniel Pacheco Bruschi http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zattera, Michelle Louise
Lima, Luana
Duarte, Iraine
de Sousa, Deborah Yasmin
Araújo, Olívia Gabriela dos Santos
Gazoni, Thiago
Mott, Tamí
Recco-Pimentel, Shirlei Maria
Bruschi, Daniel Pacheco
Chromosome spreading of the (TTAGGG)n repeats in the Pipa carvalhoi Miranda-Ribeiro, 1937 (Pipidae, Anura) karyotype
title Chromosome spreading of the (TTAGGG)n repeats in the Pipa carvalhoi Miranda-Ribeiro, 1937 (Pipidae, Anura) karyotype
title_full Chromosome spreading of the (TTAGGG)n repeats in the Pipa carvalhoi Miranda-Ribeiro, 1937 (Pipidae, Anura) karyotype
title_fullStr Chromosome spreading of the (TTAGGG)n repeats in the Pipa carvalhoi Miranda-Ribeiro, 1937 (Pipidae, Anura) karyotype
title_full_unstemmed Chromosome spreading of the (TTAGGG)n repeats in the Pipa carvalhoi Miranda-Ribeiro, 1937 (Pipidae, Anura) karyotype
title_short Chromosome spreading of the (TTAGGG)n repeats in the Pipa carvalhoi Miranda-Ribeiro, 1937 (Pipidae, Anura) karyotype
title_sort chromosome spreading of the (ttaggg)n repeats in the pipa carvalhoi miranda-ribeiro, 1937 (pipidae, anura) karyotype
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6803351/
https://www.ncbi.nlm.nih.gov/pubmed/31649799
http://dx.doi.org/10.3897/CompCytogen.v13i3.35524
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