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Histone H3K27 acetylation precedes active transcription during zebrafish zygotic genome activation as revealed by live-cell analysis
Histone post-translational modifications are key gene expression regulators, but their rapid dynamics during development remain difficult to capture. We applied a Fab-based live endogenous modification labeling technique to monitor the changes in histone modification levels during zygotic genome act...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6803375/ https://www.ncbi.nlm.nih.gov/pubmed/31570370 http://dx.doi.org/10.1242/dev.179127 |
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author | Sato, Yuko Hilbert, Lennart Oda, Haruka Wan, Yinan Heddleston, John M. Chew, Teng-Leong Zaburdaev, Vasily Keller, Philipp Lionnet, Timothee Vastenhouw, Nadine Kimura, Hiroshi |
author_facet | Sato, Yuko Hilbert, Lennart Oda, Haruka Wan, Yinan Heddleston, John M. Chew, Teng-Leong Zaburdaev, Vasily Keller, Philipp Lionnet, Timothee Vastenhouw, Nadine Kimura, Hiroshi |
author_sort | Sato, Yuko |
collection | PubMed |
description | Histone post-translational modifications are key gene expression regulators, but their rapid dynamics during development remain difficult to capture. We applied a Fab-based live endogenous modification labeling technique to monitor the changes in histone modification levels during zygotic genome activation (ZGA) in living zebrafish embryos. Among various histone modifications, H3 Lys27 acetylation (H3K27ac) exhibited most drastic changes, accumulating in two nuclear foci in the 64- to 1k-cell-stage embryos. The elongating form of RNA polymerase II, which is phosphorylated at Ser2 in heptad repeats within the C-terminal domain (RNAP2 Ser2ph), and miR-430 transcripts were also concentrated in foci closely associated with H3K27ac. When treated with α-amanitin to inhibit transcription or JQ-1 to inhibit binding of acetyl-reader proteins, H3K27ac foci still appeared but RNAP2 Ser2ph and miR-430 morpholino were not concentrated in foci, suggesting that H3K27ac precedes active transcription during ZGA. We anticipate that the method presented here could be applied to a variety of developmental processes in any model and non-model organisms. |
format | Online Article Text |
id | pubmed-6803375 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-68033752019-10-30 Histone H3K27 acetylation precedes active transcription during zebrafish zygotic genome activation as revealed by live-cell analysis Sato, Yuko Hilbert, Lennart Oda, Haruka Wan, Yinan Heddleston, John M. Chew, Teng-Leong Zaburdaev, Vasily Keller, Philipp Lionnet, Timothee Vastenhouw, Nadine Kimura, Hiroshi Development Techniques and Resources Histone post-translational modifications are key gene expression regulators, but their rapid dynamics during development remain difficult to capture. We applied a Fab-based live endogenous modification labeling technique to monitor the changes in histone modification levels during zygotic genome activation (ZGA) in living zebrafish embryos. Among various histone modifications, H3 Lys27 acetylation (H3K27ac) exhibited most drastic changes, accumulating in two nuclear foci in the 64- to 1k-cell-stage embryos. The elongating form of RNA polymerase II, which is phosphorylated at Ser2 in heptad repeats within the C-terminal domain (RNAP2 Ser2ph), and miR-430 transcripts were also concentrated in foci closely associated with H3K27ac. When treated with α-amanitin to inhibit transcription or JQ-1 to inhibit binding of acetyl-reader proteins, H3K27ac foci still appeared but RNAP2 Ser2ph and miR-430 morpholino were not concentrated in foci, suggesting that H3K27ac precedes active transcription during ZGA. We anticipate that the method presented here could be applied to a variety of developmental processes in any model and non-model organisms. The Company of Biologists Ltd 2019-10-01 2019-09-30 /pmc/articles/PMC6803375/ /pubmed/31570370 http://dx.doi.org/10.1242/dev.179127 Text en © 2019. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/4.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Techniques and Resources Sato, Yuko Hilbert, Lennart Oda, Haruka Wan, Yinan Heddleston, John M. Chew, Teng-Leong Zaburdaev, Vasily Keller, Philipp Lionnet, Timothee Vastenhouw, Nadine Kimura, Hiroshi Histone H3K27 acetylation precedes active transcription during zebrafish zygotic genome activation as revealed by live-cell analysis |
title | Histone H3K27 acetylation precedes active transcription during zebrafish zygotic genome activation as revealed by live-cell analysis |
title_full | Histone H3K27 acetylation precedes active transcription during zebrafish zygotic genome activation as revealed by live-cell analysis |
title_fullStr | Histone H3K27 acetylation precedes active transcription during zebrafish zygotic genome activation as revealed by live-cell analysis |
title_full_unstemmed | Histone H3K27 acetylation precedes active transcription during zebrafish zygotic genome activation as revealed by live-cell analysis |
title_short | Histone H3K27 acetylation precedes active transcription during zebrafish zygotic genome activation as revealed by live-cell analysis |
title_sort | histone h3k27 acetylation precedes active transcription during zebrafish zygotic genome activation as revealed by live-cell analysis |
topic | Techniques and Resources |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6803375/ https://www.ncbi.nlm.nih.gov/pubmed/31570370 http://dx.doi.org/10.1242/dev.179127 |
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