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Simulated Weightlessness Perturbs the Intestinal Metabolomic Profile of Rats
Recently, disorders of intestinal homeostasis in the space environment have been extensively demonstrated. Accumulating evidence have suggested microgravity and simulated weightlessness could induce dysbiosis of intestinal microbiota, which may contribute to the bowel symptoms during spaceflight. Ho...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6803529/ https://www.ncbi.nlm.nih.gov/pubmed/31680997 http://dx.doi.org/10.3389/fphys.2019.01279 |
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author | Jin, Mingliang Wang, Jiaojiao Zhang, Hao Zhou, Hongbin Zhao, Ke |
author_facet | Jin, Mingliang Wang, Jiaojiao Zhang, Hao Zhou, Hongbin Zhao, Ke |
author_sort | Jin, Mingliang |
collection | PubMed |
description | Recently, disorders of intestinal homeostasis in the space environment have been extensively demonstrated. Accumulating evidence have suggested microgravity and simulated weightlessness could induce dysbiosis of intestinal microbiota, which may contribute to the bowel symptoms during spaceflight. However, the specific responses of intestinal metabolome under simulated weightlessness and its relationship with the intestinal microbiome and immune characteristics remain largely unknown. In the current study, 20 adult Sprague-Dawley (SD) rats were randomly divided into the control group and the simulated weightlessness group using a hindlimb unloading model. The metabolomic profiling of cecal contents from eight rats of each group was investigated by gas chromatography-time of flight/mass spectrometry. The significantly different metabolites, biomarkers, and related pathways were identified. Multivariate analysis, such as principal component analysis and orthogonal projections to latent structures-discriminant analysis, demonstrated an obvious separation between the control group and the simulated weightlessness group. Significantly different metabolites, such as xylose, sinapinic acid, indolelactate, and digalacturonic acid, were identified, which participate in mainly pyrimidine metabolism, pentose and glucuronate interconversions, and valine, leucine and isoleucine metabolism. Cytidine-5′-monophosphate, 4-hydroxypyridine, and phloretic acid were determined as pivotal biomarkers under simulated weightlessness. Moreover, the significantly different metabolites were remarkably correlated with dysbiosis of the intestinal microbiota and disturbance of immunological characteristics induced by simulated weightlessness. These metabolic features provide crucial candidates for therapeutic targets for metabolic disorders under weightlessness. |
format | Online Article Text |
id | pubmed-6803529 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-68035292019-11-03 Simulated Weightlessness Perturbs the Intestinal Metabolomic Profile of Rats Jin, Mingliang Wang, Jiaojiao Zhang, Hao Zhou, Hongbin Zhao, Ke Front Physiol Physiology Recently, disorders of intestinal homeostasis in the space environment have been extensively demonstrated. Accumulating evidence have suggested microgravity and simulated weightlessness could induce dysbiosis of intestinal microbiota, which may contribute to the bowel symptoms during spaceflight. However, the specific responses of intestinal metabolome under simulated weightlessness and its relationship with the intestinal microbiome and immune characteristics remain largely unknown. In the current study, 20 adult Sprague-Dawley (SD) rats were randomly divided into the control group and the simulated weightlessness group using a hindlimb unloading model. The metabolomic profiling of cecal contents from eight rats of each group was investigated by gas chromatography-time of flight/mass spectrometry. The significantly different metabolites, biomarkers, and related pathways were identified. Multivariate analysis, such as principal component analysis and orthogonal projections to latent structures-discriminant analysis, demonstrated an obvious separation between the control group and the simulated weightlessness group. Significantly different metabolites, such as xylose, sinapinic acid, indolelactate, and digalacturonic acid, were identified, which participate in mainly pyrimidine metabolism, pentose and glucuronate interconversions, and valine, leucine and isoleucine metabolism. Cytidine-5′-monophosphate, 4-hydroxypyridine, and phloretic acid were determined as pivotal biomarkers under simulated weightlessness. Moreover, the significantly different metabolites were remarkably correlated with dysbiosis of the intestinal microbiota and disturbance of immunological characteristics induced by simulated weightlessness. These metabolic features provide crucial candidates for therapeutic targets for metabolic disorders under weightlessness. Frontiers Media S.A. 2019-10-15 /pmc/articles/PMC6803529/ /pubmed/31680997 http://dx.doi.org/10.3389/fphys.2019.01279 Text en Copyright © 2019 Jin, Wang, Zhang, Zhou and Zhao. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Jin, Mingliang Wang, Jiaojiao Zhang, Hao Zhou, Hongbin Zhao, Ke Simulated Weightlessness Perturbs the Intestinal Metabolomic Profile of Rats |
title | Simulated Weightlessness Perturbs the Intestinal Metabolomic Profile of Rats |
title_full | Simulated Weightlessness Perturbs the Intestinal Metabolomic Profile of Rats |
title_fullStr | Simulated Weightlessness Perturbs the Intestinal Metabolomic Profile of Rats |
title_full_unstemmed | Simulated Weightlessness Perturbs the Intestinal Metabolomic Profile of Rats |
title_short | Simulated Weightlessness Perturbs the Intestinal Metabolomic Profile of Rats |
title_sort | simulated weightlessness perturbs the intestinal metabolomic profile of rats |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6803529/ https://www.ncbi.nlm.nih.gov/pubmed/31680997 http://dx.doi.org/10.3389/fphys.2019.01279 |
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