A Convenient Fluorescence-Based Assay for the Detection of Sucrose Transport and the Introduction of a Sucrose Transporter from Potato into Clostridium Strains

A convenient and effective sucrose transport assay for Clostridium strains is needed. Traditional methods, such as (14)C-sucrose isotope labelling, use radioactive materials and are not convenient for many laboratories. Here, a sucrose transporter from potato was introduced into Clostridium, and a f...

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Autores principales: Zhang, Zhikai, Lin, Lihua, Tang, Hongchi, Zeng, Shaowei, Guo, Yuan, Wei, Yutuo, Huang, Ribo, Pang, Hao, Du, Liqin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6803915/
https://www.ncbi.nlm.nih.gov/pubmed/31561523
http://dx.doi.org/10.3390/molecules24193495
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author Zhang, Zhikai
Lin, Lihua
Tang, Hongchi
Zeng, Shaowei
Guo, Yuan
Wei, Yutuo
Huang, Ribo
Pang, Hao
Du, Liqin
author_facet Zhang, Zhikai
Lin, Lihua
Tang, Hongchi
Zeng, Shaowei
Guo, Yuan
Wei, Yutuo
Huang, Ribo
Pang, Hao
Du, Liqin
author_sort Zhang, Zhikai
collection PubMed
description A convenient and effective sucrose transport assay for Clostridium strains is needed. Traditional methods, such as (14)C-sucrose isotope labelling, use radioactive materials and are not convenient for many laboratories. Here, a sucrose transporter from potato was introduced into Clostridium, and a fluorescence assay based on esculin was used for the analysis of sucrose transport in Clostridium strains. This showed that the heterologously expressed potato sucrose transporter is functional in Clostridium. Recombinant engineering of high-level sucrose transport would aid sucrose fermentation in Clostridium strains. The assay described herein provides an important technological platform for studying sucrose transporter function following heterologous expression in Clostridium.
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spelling pubmed-68039152019-11-18 A Convenient Fluorescence-Based Assay for the Detection of Sucrose Transport and the Introduction of a Sucrose Transporter from Potato into Clostridium Strains Zhang, Zhikai Lin, Lihua Tang, Hongchi Zeng, Shaowei Guo, Yuan Wei, Yutuo Huang, Ribo Pang, Hao Du, Liqin Molecules Article A convenient and effective sucrose transport assay for Clostridium strains is needed. Traditional methods, such as (14)C-sucrose isotope labelling, use radioactive materials and are not convenient for many laboratories. Here, a sucrose transporter from potato was introduced into Clostridium, and a fluorescence assay based on esculin was used for the analysis of sucrose transport in Clostridium strains. This showed that the heterologously expressed potato sucrose transporter is functional in Clostridium. Recombinant engineering of high-level sucrose transport would aid sucrose fermentation in Clostridium strains. The assay described herein provides an important technological platform for studying sucrose transporter function following heterologous expression in Clostridium. MDPI 2019-09-26 /pmc/articles/PMC6803915/ /pubmed/31561523 http://dx.doi.org/10.3390/molecules24193495 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Zhikai
Lin, Lihua
Tang, Hongchi
Zeng, Shaowei
Guo, Yuan
Wei, Yutuo
Huang, Ribo
Pang, Hao
Du, Liqin
A Convenient Fluorescence-Based Assay for the Detection of Sucrose Transport and the Introduction of a Sucrose Transporter from Potato into Clostridium Strains
title A Convenient Fluorescence-Based Assay for the Detection of Sucrose Transport and the Introduction of a Sucrose Transporter from Potato into Clostridium Strains
title_full A Convenient Fluorescence-Based Assay for the Detection of Sucrose Transport and the Introduction of a Sucrose Transporter from Potato into Clostridium Strains
title_fullStr A Convenient Fluorescence-Based Assay for the Detection of Sucrose Transport and the Introduction of a Sucrose Transporter from Potato into Clostridium Strains
title_full_unstemmed A Convenient Fluorescence-Based Assay for the Detection of Sucrose Transport and the Introduction of a Sucrose Transporter from Potato into Clostridium Strains
title_short A Convenient Fluorescence-Based Assay for the Detection of Sucrose Transport and the Introduction of a Sucrose Transporter from Potato into Clostridium Strains
title_sort convenient fluorescence-based assay for the detection of sucrose transport and the introduction of a sucrose transporter from potato into clostridium strains
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6803915/
https://www.ncbi.nlm.nih.gov/pubmed/31561523
http://dx.doi.org/10.3390/molecules24193495
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