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Differential Mechanism of ATP Production Occurs in Response to Succinylacetone in Colon Cancer Cells

Our aim was to verify the potential ability of succinylacetone (SA) to inhibit mitochondrial function, thereby suppressing cancer cell proliferation. SA treatment caused apoptosis in HCT116 and HT29 cells, but not in SW480 cells, with mitochondria playing a key role. We checked for dysfunctional mit...

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Detalles Bibliográficos
Autores principales: Lee, Phil Jun, Woo, Seung Je, Yoo, Hee Min, Cho, Namki, Kim, Hong Pyo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6803967/
https://www.ncbi.nlm.nih.gov/pubmed/31623369
http://dx.doi.org/10.3390/molecules24193575
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author Lee, Phil Jun
Woo, Seung Je
Yoo, Hee Min
Cho, Namki
Kim, Hong Pyo
author_facet Lee, Phil Jun
Woo, Seung Je
Yoo, Hee Min
Cho, Namki
Kim, Hong Pyo
author_sort Lee, Phil Jun
collection PubMed
description Our aim was to verify the potential ability of succinylacetone (SA) to inhibit mitochondrial function, thereby suppressing cancer cell proliferation. SA treatment caused apoptosis in HCT116 and HT29 cells, but not in SW480 cells, with mitochondria playing a key role. We checked for dysfunctional mitochondria after SA treatment. Mitochondria of HT29 cells were swollen, indicating damage, whereas in HCT116 cells, several mitochondria had a diminished size. Damaged mitochondria decreased ATP production and induced reactive oxygen species (ROS) in the cells. To understand SA-induced reduction in ATP production, we investigated the electron transfer chains (ETC) and pyruvate dehydrogenase kinase (PDK) activity, which prevents the transfer of acetyl-CoA to the TCA (tricarboxylic acid) cycle by inhibiting PDH (pyruvate dehydrogenase) activity. In each cell line, the inhibitory mechanism of ATP by SA was different. The activity of complex III consisting of the mitochondrial ETCs in HT29 cells was decreased. In contrast, PDH activity in HCT116 cells was reduced. Nicotinamide nucleotide transhydrogenase (NNT)-removing reactive oxygen species (ROS) was upregulated in HT29 cells, but not in HCT116 cells, indicating that in HT29 cells, a defense mechanism was activated against ROS. Collectively, our study showed a differential mechanism occurs in response to SA in colon cancer cells.
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spelling pubmed-68039672019-11-18 Differential Mechanism of ATP Production Occurs in Response to Succinylacetone in Colon Cancer Cells Lee, Phil Jun Woo, Seung Je Yoo, Hee Min Cho, Namki Kim, Hong Pyo Molecules Article Our aim was to verify the potential ability of succinylacetone (SA) to inhibit mitochondrial function, thereby suppressing cancer cell proliferation. SA treatment caused apoptosis in HCT116 and HT29 cells, but not in SW480 cells, with mitochondria playing a key role. We checked for dysfunctional mitochondria after SA treatment. Mitochondria of HT29 cells were swollen, indicating damage, whereas in HCT116 cells, several mitochondria had a diminished size. Damaged mitochondria decreased ATP production and induced reactive oxygen species (ROS) in the cells. To understand SA-induced reduction in ATP production, we investigated the electron transfer chains (ETC) and pyruvate dehydrogenase kinase (PDK) activity, which prevents the transfer of acetyl-CoA to the TCA (tricarboxylic acid) cycle by inhibiting PDH (pyruvate dehydrogenase) activity. In each cell line, the inhibitory mechanism of ATP by SA was different. The activity of complex III consisting of the mitochondrial ETCs in HT29 cells was decreased. In contrast, PDH activity in HCT116 cells was reduced. Nicotinamide nucleotide transhydrogenase (NNT)-removing reactive oxygen species (ROS) was upregulated in HT29 cells, but not in HCT116 cells, indicating that in HT29 cells, a defense mechanism was activated against ROS. Collectively, our study showed a differential mechanism occurs in response to SA in colon cancer cells. MDPI 2019-10-03 /pmc/articles/PMC6803967/ /pubmed/31623369 http://dx.doi.org/10.3390/molecules24193575 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Phil Jun
Woo, Seung Je
Yoo, Hee Min
Cho, Namki
Kim, Hong Pyo
Differential Mechanism of ATP Production Occurs in Response to Succinylacetone in Colon Cancer Cells
title Differential Mechanism of ATP Production Occurs in Response to Succinylacetone in Colon Cancer Cells
title_full Differential Mechanism of ATP Production Occurs in Response to Succinylacetone in Colon Cancer Cells
title_fullStr Differential Mechanism of ATP Production Occurs in Response to Succinylacetone in Colon Cancer Cells
title_full_unstemmed Differential Mechanism of ATP Production Occurs in Response to Succinylacetone in Colon Cancer Cells
title_short Differential Mechanism of ATP Production Occurs in Response to Succinylacetone in Colon Cancer Cells
title_sort differential mechanism of atp production occurs in response to succinylacetone in colon cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6803967/
https://www.ncbi.nlm.nih.gov/pubmed/31623369
http://dx.doi.org/10.3390/molecules24193575
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