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Matrix Discriminant Analysis Evidenced Surface-Lithium as an Important Factor to Increase the Hydrolytic Saccharification of Sugarcane Bagasse
Statistical evidence pointing to the very soft change in the ionic composition on the surface of the sugar cane bagasse is crucial to improve yields of sugars by hydrolytic saccharification. Removal of Li(+) by pretreatments exposing -OH sites was the most important factor related to the increase of...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804010/ https://www.ncbi.nlm.nih.gov/pubmed/31597244 http://dx.doi.org/10.3390/molecules24193614 |
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author | de Almeida Scarcella, Ana Sílvia Somera, Alexandre Favarin da Costa Carreira Nunes, Christiane Gomes, Eleni Vici, Ana Claudia Buckeridge, Marcos Silveira de Moraes Polizeli, Maria de Lourdes Teixeira |
author_facet | de Almeida Scarcella, Ana Sílvia Somera, Alexandre Favarin da Costa Carreira Nunes, Christiane Gomes, Eleni Vici, Ana Claudia Buckeridge, Marcos Silveira de Moraes Polizeli, Maria de Lourdes Teixeira |
author_sort | de Almeida Scarcella, Ana Sílvia |
collection | PubMed |
description | Statistical evidence pointing to the very soft change in the ionic composition on the surface of the sugar cane bagasse is crucial to improve yields of sugars by hydrolytic saccharification. Removal of Li(+) by pretreatments exposing -OH sites was the most important factor related to the increase of saccharification yields using enzyme cocktails. Steam Explosion and Microwave:H(2)SO(4) pretreatments produced unrelated structural changes, but similar ionic distribution patterns. Both increased the saccharification yield 1.74-fold. NaOH produced structural changes related to Steam Explosion, but released surface-bounded Li(+) obtaining 2.04-fold more reducing sugars than the control. In turn, the higher amounts in relative concentration and periodic structures of Li(+) on the surface observed in the control or after the pretreatment with Ethanol:DMSO:Ammonium Oxalate, blocked -OH and O(−) available for ionic sputtering. These changes correlated to 1.90-fold decrease in saccharification yields. Li(+) was an activator in solution, but its presence and distribution pattern on the substrate was prejudicial to the saccharification. Apparently, it acts as a phase-dependent modulator of enzyme activity. Therefore, no correlations were found between structural changes and the efficiency of the enzymatic cocktail used. However, there were correlations between the Li(+) distribution patterns and the enzymatic activities that should to be shown. |
format | Online Article Text |
id | pubmed-6804010 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68040102019-11-18 Matrix Discriminant Analysis Evidenced Surface-Lithium as an Important Factor to Increase the Hydrolytic Saccharification of Sugarcane Bagasse de Almeida Scarcella, Ana Sílvia Somera, Alexandre Favarin da Costa Carreira Nunes, Christiane Gomes, Eleni Vici, Ana Claudia Buckeridge, Marcos Silveira de Moraes Polizeli, Maria de Lourdes Teixeira Molecules Article Statistical evidence pointing to the very soft change in the ionic composition on the surface of the sugar cane bagasse is crucial to improve yields of sugars by hydrolytic saccharification. Removal of Li(+) by pretreatments exposing -OH sites was the most important factor related to the increase of saccharification yields using enzyme cocktails. Steam Explosion and Microwave:H(2)SO(4) pretreatments produced unrelated structural changes, but similar ionic distribution patterns. Both increased the saccharification yield 1.74-fold. NaOH produced structural changes related to Steam Explosion, but released surface-bounded Li(+) obtaining 2.04-fold more reducing sugars than the control. In turn, the higher amounts in relative concentration and periodic structures of Li(+) on the surface observed in the control or after the pretreatment with Ethanol:DMSO:Ammonium Oxalate, blocked -OH and O(−) available for ionic sputtering. These changes correlated to 1.90-fold decrease in saccharification yields. Li(+) was an activator in solution, but its presence and distribution pattern on the substrate was prejudicial to the saccharification. Apparently, it acts as a phase-dependent modulator of enzyme activity. Therefore, no correlations were found between structural changes and the efficiency of the enzymatic cocktail used. However, there were correlations between the Li(+) distribution patterns and the enzymatic activities that should to be shown. MDPI 2019-10-08 /pmc/articles/PMC6804010/ /pubmed/31597244 http://dx.doi.org/10.3390/molecules24193614 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article de Almeida Scarcella, Ana Sílvia Somera, Alexandre Favarin da Costa Carreira Nunes, Christiane Gomes, Eleni Vici, Ana Claudia Buckeridge, Marcos Silveira de Moraes Polizeli, Maria de Lourdes Teixeira Matrix Discriminant Analysis Evidenced Surface-Lithium as an Important Factor to Increase the Hydrolytic Saccharification of Sugarcane Bagasse |
title | Matrix Discriminant Analysis Evidenced Surface-Lithium as an Important Factor to Increase the Hydrolytic Saccharification of Sugarcane Bagasse |
title_full | Matrix Discriminant Analysis Evidenced Surface-Lithium as an Important Factor to Increase the Hydrolytic Saccharification of Sugarcane Bagasse |
title_fullStr | Matrix Discriminant Analysis Evidenced Surface-Lithium as an Important Factor to Increase the Hydrolytic Saccharification of Sugarcane Bagasse |
title_full_unstemmed | Matrix Discriminant Analysis Evidenced Surface-Lithium as an Important Factor to Increase the Hydrolytic Saccharification of Sugarcane Bagasse |
title_short | Matrix Discriminant Analysis Evidenced Surface-Lithium as an Important Factor to Increase the Hydrolytic Saccharification of Sugarcane Bagasse |
title_sort | matrix discriminant analysis evidenced surface-lithium as an important factor to increase the hydrolytic saccharification of sugarcane bagasse |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804010/ https://www.ncbi.nlm.nih.gov/pubmed/31597244 http://dx.doi.org/10.3390/molecules24193614 |
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