Cargando…
Anisotropic Cryostructured Collagen Scaffolds for Efficient Delivery of RhBMP–2 and Enhanced Bone Regeneration
In the treatment of bone non-unions, an alternative to bone autografts is the use of bone morphogenetic proteins (BMPs), e.g., BMP–2, BMP–7, with powerful osteoinductive and osteogenic properties. In clinical settings, these osteogenic factors are applied using absorbable collagen sponges for local...
Autores principales: | , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804013/ https://www.ncbi.nlm.nih.gov/pubmed/31554158 http://dx.doi.org/10.3390/ma12193105 |
_version_ | 1783461083153956864 |
---|---|
author | Stuckensen, Kai Lamo-Espinosa, José M. Muiños-López, Emma Ripalda-Cemboráin, Purificación López-Martínez, Tania Iglesias, Elena Abizanda, Gloria Andreu, Ion Flandes-Iparraguirre, María Pons-Villanueva, Juan Elizalde, Reyes Nickel, Joachim Ewald, Andrea Gbureck, Uwe Prósper, Felipe Groll, Jürgen Granero-Moltó, Froilán |
author_facet | Stuckensen, Kai Lamo-Espinosa, José M. Muiños-López, Emma Ripalda-Cemboráin, Purificación López-Martínez, Tania Iglesias, Elena Abizanda, Gloria Andreu, Ion Flandes-Iparraguirre, María Pons-Villanueva, Juan Elizalde, Reyes Nickel, Joachim Ewald, Andrea Gbureck, Uwe Prósper, Felipe Groll, Jürgen Granero-Moltó, Froilán |
author_sort | Stuckensen, Kai |
collection | PubMed |
description | In the treatment of bone non-unions, an alternative to bone autografts is the use of bone morphogenetic proteins (BMPs), e.g., BMP–2, BMP–7, with powerful osteoinductive and osteogenic properties. In clinical settings, these osteogenic factors are applied using absorbable collagen sponges for local controlled delivery. Major side effects of this strategy are derived from the supraphysiological doses of BMPs needed, which may induce ectopic bone formation, chronic inflammation, and excessive bone resorption. In order to increase the efficiency of the delivered BMPs, we designed cryostructured collagen scaffolds functionalized with hydroxyapatite, mimicking the structure of cortical bone (aligned porosity, anisotropic) or trabecular bone (random distributed porosity, isotropic). We hypothesize that an anisotropic structure would enhance the osteoconductive properties of the scaffolds by increasing the regenerative performance of the provided rhBMP–2. In vitro, both scaffolds presented similar mechanical properties, rhBMP–2 retention and delivery capacity, as well as scaffold degradation time. In vivo, anisotropic scaffolds demonstrated better bone regeneration capabilities in a rat femoral critical-size defect model by increasing the defect bridging. In conclusion, anisotropic cryostructured collagen scaffolds improve bone regeneration by increasing the efficiency of rhBMP–2 mediated bone healing. |
format | Online Article Text |
id | pubmed-6804013 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68040132019-11-18 Anisotropic Cryostructured Collagen Scaffolds for Efficient Delivery of RhBMP–2 and Enhanced Bone Regeneration Stuckensen, Kai Lamo-Espinosa, José M. Muiños-López, Emma Ripalda-Cemboráin, Purificación López-Martínez, Tania Iglesias, Elena Abizanda, Gloria Andreu, Ion Flandes-Iparraguirre, María Pons-Villanueva, Juan Elizalde, Reyes Nickel, Joachim Ewald, Andrea Gbureck, Uwe Prósper, Felipe Groll, Jürgen Granero-Moltó, Froilán Materials (Basel) Article In the treatment of bone non-unions, an alternative to bone autografts is the use of bone morphogenetic proteins (BMPs), e.g., BMP–2, BMP–7, with powerful osteoinductive and osteogenic properties. In clinical settings, these osteogenic factors are applied using absorbable collagen sponges for local controlled delivery. Major side effects of this strategy are derived from the supraphysiological doses of BMPs needed, which may induce ectopic bone formation, chronic inflammation, and excessive bone resorption. In order to increase the efficiency of the delivered BMPs, we designed cryostructured collagen scaffolds functionalized with hydroxyapatite, mimicking the structure of cortical bone (aligned porosity, anisotropic) or trabecular bone (random distributed porosity, isotropic). We hypothesize that an anisotropic structure would enhance the osteoconductive properties of the scaffolds by increasing the regenerative performance of the provided rhBMP–2. In vitro, both scaffolds presented similar mechanical properties, rhBMP–2 retention and delivery capacity, as well as scaffold degradation time. In vivo, anisotropic scaffolds demonstrated better bone regeneration capabilities in a rat femoral critical-size defect model by increasing the defect bridging. In conclusion, anisotropic cryostructured collagen scaffolds improve bone regeneration by increasing the efficiency of rhBMP–2 mediated bone healing. MDPI 2019-09-24 /pmc/articles/PMC6804013/ /pubmed/31554158 http://dx.doi.org/10.3390/ma12193105 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Stuckensen, Kai Lamo-Espinosa, José M. Muiños-López, Emma Ripalda-Cemboráin, Purificación López-Martínez, Tania Iglesias, Elena Abizanda, Gloria Andreu, Ion Flandes-Iparraguirre, María Pons-Villanueva, Juan Elizalde, Reyes Nickel, Joachim Ewald, Andrea Gbureck, Uwe Prósper, Felipe Groll, Jürgen Granero-Moltó, Froilán Anisotropic Cryostructured Collagen Scaffolds for Efficient Delivery of RhBMP–2 and Enhanced Bone Regeneration |
title | Anisotropic Cryostructured Collagen Scaffolds for Efficient Delivery of RhBMP–2 and Enhanced Bone Regeneration |
title_full | Anisotropic Cryostructured Collagen Scaffolds for Efficient Delivery of RhBMP–2 and Enhanced Bone Regeneration |
title_fullStr | Anisotropic Cryostructured Collagen Scaffolds for Efficient Delivery of RhBMP–2 and Enhanced Bone Regeneration |
title_full_unstemmed | Anisotropic Cryostructured Collagen Scaffolds for Efficient Delivery of RhBMP–2 and Enhanced Bone Regeneration |
title_short | Anisotropic Cryostructured Collagen Scaffolds for Efficient Delivery of RhBMP–2 and Enhanced Bone Regeneration |
title_sort | anisotropic cryostructured collagen scaffolds for efficient delivery of rhbmp–2 and enhanced bone regeneration |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804013/ https://www.ncbi.nlm.nih.gov/pubmed/31554158 http://dx.doi.org/10.3390/ma12193105 |
work_keys_str_mv | AT stuckensenkai anisotropiccryostructuredcollagenscaffoldsforefficientdeliveryofrhbmp2andenhancedboneregeneration AT lamoespinosajosem anisotropiccryostructuredcollagenscaffoldsforefficientdeliveryofrhbmp2andenhancedboneregeneration AT muinoslopezemma anisotropiccryostructuredcollagenscaffoldsforefficientdeliveryofrhbmp2andenhancedboneregeneration AT ripaldacemborainpurificacion anisotropiccryostructuredcollagenscaffoldsforefficientdeliveryofrhbmp2andenhancedboneregeneration AT lopezmartineztania anisotropiccryostructuredcollagenscaffoldsforefficientdeliveryofrhbmp2andenhancedboneregeneration AT iglesiaselena anisotropiccryostructuredcollagenscaffoldsforefficientdeliveryofrhbmp2andenhancedboneregeneration AT abizandagloria anisotropiccryostructuredcollagenscaffoldsforefficientdeliveryofrhbmp2andenhancedboneregeneration AT andreuion anisotropiccryostructuredcollagenscaffoldsforefficientdeliveryofrhbmp2andenhancedboneregeneration AT flandesiparraguirremaria anisotropiccryostructuredcollagenscaffoldsforefficientdeliveryofrhbmp2andenhancedboneregeneration AT ponsvillanuevajuan anisotropiccryostructuredcollagenscaffoldsforefficientdeliveryofrhbmp2andenhancedboneregeneration AT elizaldereyes anisotropiccryostructuredcollagenscaffoldsforefficientdeliveryofrhbmp2andenhancedboneregeneration AT nickeljoachim anisotropiccryostructuredcollagenscaffoldsforefficientdeliveryofrhbmp2andenhancedboneregeneration AT ewaldandrea anisotropiccryostructuredcollagenscaffoldsforefficientdeliveryofrhbmp2andenhancedboneregeneration AT gbureckuwe anisotropiccryostructuredcollagenscaffoldsforefficientdeliveryofrhbmp2andenhancedboneregeneration AT prosperfelipe anisotropiccryostructuredcollagenscaffoldsforefficientdeliveryofrhbmp2andenhancedboneregeneration AT grolljurgen anisotropiccryostructuredcollagenscaffoldsforefficientdeliveryofrhbmp2andenhancedboneregeneration AT graneromoltofroilan anisotropiccryostructuredcollagenscaffoldsforefficientdeliveryofrhbmp2andenhancedboneregeneration |