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Anisotropic Cryostructured Collagen Scaffolds for Efficient Delivery of RhBMP–2 and Enhanced Bone Regeneration

In the treatment of bone non-unions, an alternative to bone autografts is the use of bone morphogenetic proteins (BMPs), e.g., BMP–2, BMP–7, with powerful osteoinductive and osteogenic properties. In clinical settings, these osteogenic factors are applied using absorbable collagen sponges for local...

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Autores principales: Stuckensen, Kai, Lamo-Espinosa, José M., Muiños-López, Emma, Ripalda-Cemboráin, Purificación, López-Martínez, Tania, Iglesias, Elena, Abizanda, Gloria, Andreu, Ion, Flandes-Iparraguirre, María, Pons-Villanueva, Juan, Elizalde, Reyes, Nickel, Joachim, Ewald, Andrea, Gbureck, Uwe, Prósper, Felipe, Groll, Jürgen, Granero-Moltó, Froilán
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804013/
https://www.ncbi.nlm.nih.gov/pubmed/31554158
http://dx.doi.org/10.3390/ma12193105
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author Stuckensen, Kai
Lamo-Espinosa, José M.
Muiños-López, Emma
Ripalda-Cemboráin, Purificación
López-Martínez, Tania
Iglesias, Elena
Abizanda, Gloria
Andreu, Ion
Flandes-Iparraguirre, María
Pons-Villanueva, Juan
Elizalde, Reyes
Nickel, Joachim
Ewald, Andrea
Gbureck, Uwe
Prósper, Felipe
Groll, Jürgen
Granero-Moltó, Froilán
author_facet Stuckensen, Kai
Lamo-Espinosa, José M.
Muiños-López, Emma
Ripalda-Cemboráin, Purificación
López-Martínez, Tania
Iglesias, Elena
Abizanda, Gloria
Andreu, Ion
Flandes-Iparraguirre, María
Pons-Villanueva, Juan
Elizalde, Reyes
Nickel, Joachim
Ewald, Andrea
Gbureck, Uwe
Prósper, Felipe
Groll, Jürgen
Granero-Moltó, Froilán
author_sort Stuckensen, Kai
collection PubMed
description In the treatment of bone non-unions, an alternative to bone autografts is the use of bone morphogenetic proteins (BMPs), e.g., BMP–2, BMP–7, with powerful osteoinductive and osteogenic properties. In clinical settings, these osteogenic factors are applied using absorbable collagen sponges for local controlled delivery. Major side effects of this strategy are derived from the supraphysiological doses of BMPs needed, which may induce ectopic bone formation, chronic inflammation, and excessive bone resorption. In order to increase the efficiency of the delivered BMPs, we designed cryostructured collagen scaffolds functionalized with hydroxyapatite, mimicking the structure of cortical bone (aligned porosity, anisotropic) or trabecular bone (random distributed porosity, isotropic). We hypothesize that an anisotropic structure would enhance the osteoconductive properties of the scaffolds by increasing the regenerative performance of the provided rhBMP–2. In vitro, both scaffolds presented similar mechanical properties, rhBMP–2 retention and delivery capacity, as well as scaffold degradation time. In vivo, anisotropic scaffolds demonstrated better bone regeneration capabilities in a rat femoral critical-size defect model by increasing the defect bridging. In conclusion, anisotropic cryostructured collagen scaffolds improve bone regeneration by increasing the efficiency of rhBMP–2 mediated bone healing.
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spelling pubmed-68040132019-11-18 Anisotropic Cryostructured Collagen Scaffolds for Efficient Delivery of RhBMP–2 and Enhanced Bone Regeneration Stuckensen, Kai Lamo-Espinosa, José M. Muiños-López, Emma Ripalda-Cemboráin, Purificación López-Martínez, Tania Iglesias, Elena Abizanda, Gloria Andreu, Ion Flandes-Iparraguirre, María Pons-Villanueva, Juan Elizalde, Reyes Nickel, Joachim Ewald, Andrea Gbureck, Uwe Prósper, Felipe Groll, Jürgen Granero-Moltó, Froilán Materials (Basel) Article In the treatment of bone non-unions, an alternative to bone autografts is the use of bone morphogenetic proteins (BMPs), e.g., BMP–2, BMP–7, with powerful osteoinductive and osteogenic properties. In clinical settings, these osteogenic factors are applied using absorbable collagen sponges for local controlled delivery. Major side effects of this strategy are derived from the supraphysiological doses of BMPs needed, which may induce ectopic bone formation, chronic inflammation, and excessive bone resorption. In order to increase the efficiency of the delivered BMPs, we designed cryostructured collagen scaffolds functionalized with hydroxyapatite, mimicking the structure of cortical bone (aligned porosity, anisotropic) or trabecular bone (random distributed porosity, isotropic). We hypothesize that an anisotropic structure would enhance the osteoconductive properties of the scaffolds by increasing the regenerative performance of the provided rhBMP–2. In vitro, both scaffolds presented similar mechanical properties, rhBMP–2 retention and delivery capacity, as well as scaffold degradation time. In vivo, anisotropic scaffolds demonstrated better bone regeneration capabilities in a rat femoral critical-size defect model by increasing the defect bridging. In conclusion, anisotropic cryostructured collagen scaffolds improve bone regeneration by increasing the efficiency of rhBMP–2 mediated bone healing. MDPI 2019-09-24 /pmc/articles/PMC6804013/ /pubmed/31554158 http://dx.doi.org/10.3390/ma12193105 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Stuckensen, Kai
Lamo-Espinosa, José M.
Muiños-López, Emma
Ripalda-Cemboráin, Purificación
López-Martínez, Tania
Iglesias, Elena
Abizanda, Gloria
Andreu, Ion
Flandes-Iparraguirre, María
Pons-Villanueva, Juan
Elizalde, Reyes
Nickel, Joachim
Ewald, Andrea
Gbureck, Uwe
Prósper, Felipe
Groll, Jürgen
Granero-Moltó, Froilán
Anisotropic Cryostructured Collagen Scaffolds for Efficient Delivery of RhBMP–2 and Enhanced Bone Regeneration
title Anisotropic Cryostructured Collagen Scaffolds for Efficient Delivery of RhBMP–2 and Enhanced Bone Regeneration
title_full Anisotropic Cryostructured Collagen Scaffolds for Efficient Delivery of RhBMP–2 and Enhanced Bone Regeneration
title_fullStr Anisotropic Cryostructured Collagen Scaffolds for Efficient Delivery of RhBMP–2 and Enhanced Bone Regeneration
title_full_unstemmed Anisotropic Cryostructured Collagen Scaffolds for Efficient Delivery of RhBMP–2 and Enhanced Bone Regeneration
title_short Anisotropic Cryostructured Collagen Scaffolds for Efficient Delivery of RhBMP–2 and Enhanced Bone Regeneration
title_sort anisotropic cryostructured collagen scaffolds for efficient delivery of rhbmp–2 and enhanced bone regeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804013/
https://www.ncbi.nlm.nih.gov/pubmed/31554158
http://dx.doi.org/10.3390/ma12193105
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