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Comparative Cytocompatibility and Mineralization Potential of Bio-C Sealer and TotalFill BC Sealer
The aim of this study was to investigate the cytocompatibility and mineralization potential of two premixed hydraulic endodontic sealers compared with an epoxy resin-based root canal sealer. The cellular responses and mineralization capacity were studied in human periodontal ligament stem cells (hPD...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804055/ https://www.ncbi.nlm.nih.gov/pubmed/31546696 http://dx.doi.org/10.3390/ma12193087 |
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author | López-García, Sergio Pecci-Lloret, Miguel R. Guerrero-Gironés, Julia Pecci-Lloret, María P. Lozano, Adrián Llena, Carmen Rodríguez-Lozano, Francisco Javier Forner, Leopoldo |
author_facet | López-García, Sergio Pecci-Lloret, Miguel R. Guerrero-Gironés, Julia Pecci-Lloret, María P. Lozano, Adrián Llena, Carmen Rodríguez-Lozano, Francisco Javier Forner, Leopoldo |
author_sort | López-García, Sergio |
collection | PubMed |
description | The aim of this study was to investigate the cytocompatibility and mineralization potential of two premixed hydraulic endodontic sealers compared with an epoxy resin-based root canal sealer. The cellular responses and mineralization capacity were studied in human periodontal ligament stem cells (hPDLSCs) that were exposed to premixed hydraulic sealers, Bio-C Sealer (Angelus, Londrína, PR, Brazil), TotalFill BC Sealer (FKG Dentaire SA, La-Chaux-de-fonds, Switzerland) and an epoxy resin-based material, AH Plus (Dentsply De Trey, Konstanz, Germany). Non-exposed cultures served as the control. The endodontic sealers were assessed using scanning electron microscopy (SEM) and energy dispersive X-ray microanalysis (EDX). Statistical analyses were done using Analisis of Variance (ANOVA), with Bonferroni adjusted pairwise comparison (p = 0.05). AH Plus reduced cell viability and cell migration, whereas increased cell viability and cell migration were observed in the Bio-C Sealer and the TotalFill BC Sealer (p < 0.05). The lowest cell attachment and spreading were observed for all concentrations of AH Plus, whereas the highest were observed for TotalFill BC Sealer. At the end of 21 days, only the Bio-C Sealer and the TotalFill BC Sealer supported matrix mineralization (p < 0.05). Additionally, SEM-EDX revealed high content of calcium, oxygen, and silicon in the Bio-C Sealer and the TotalFill BC Sealer. Based on the results from this study, Bio-C Sealer and TotalFill BC Sealer demonstrated better cytocompatibility in terms of cell viability, migration, cell morphology, cell attachment, and mineralization capacity than AH Plus. |
format | Online Article Text |
id | pubmed-6804055 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68040552019-11-18 Comparative Cytocompatibility and Mineralization Potential of Bio-C Sealer and TotalFill BC Sealer López-García, Sergio Pecci-Lloret, Miguel R. Guerrero-Gironés, Julia Pecci-Lloret, María P. Lozano, Adrián Llena, Carmen Rodríguez-Lozano, Francisco Javier Forner, Leopoldo Materials (Basel) Article The aim of this study was to investigate the cytocompatibility and mineralization potential of two premixed hydraulic endodontic sealers compared with an epoxy resin-based root canal sealer. The cellular responses and mineralization capacity were studied in human periodontal ligament stem cells (hPDLSCs) that were exposed to premixed hydraulic sealers, Bio-C Sealer (Angelus, Londrína, PR, Brazil), TotalFill BC Sealer (FKG Dentaire SA, La-Chaux-de-fonds, Switzerland) and an epoxy resin-based material, AH Plus (Dentsply De Trey, Konstanz, Germany). Non-exposed cultures served as the control. The endodontic sealers were assessed using scanning electron microscopy (SEM) and energy dispersive X-ray microanalysis (EDX). Statistical analyses were done using Analisis of Variance (ANOVA), with Bonferroni adjusted pairwise comparison (p = 0.05). AH Plus reduced cell viability and cell migration, whereas increased cell viability and cell migration were observed in the Bio-C Sealer and the TotalFill BC Sealer (p < 0.05). The lowest cell attachment and spreading were observed for all concentrations of AH Plus, whereas the highest were observed for TotalFill BC Sealer. At the end of 21 days, only the Bio-C Sealer and the TotalFill BC Sealer supported matrix mineralization (p < 0.05). Additionally, SEM-EDX revealed high content of calcium, oxygen, and silicon in the Bio-C Sealer and the TotalFill BC Sealer. Based on the results from this study, Bio-C Sealer and TotalFill BC Sealer demonstrated better cytocompatibility in terms of cell viability, migration, cell morphology, cell attachment, and mineralization capacity than AH Plus. MDPI 2019-09-22 /pmc/articles/PMC6804055/ /pubmed/31546696 http://dx.doi.org/10.3390/ma12193087 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article López-García, Sergio Pecci-Lloret, Miguel R. Guerrero-Gironés, Julia Pecci-Lloret, María P. Lozano, Adrián Llena, Carmen Rodríguez-Lozano, Francisco Javier Forner, Leopoldo Comparative Cytocompatibility and Mineralization Potential of Bio-C Sealer and TotalFill BC Sealer |
title | Comparative Cytocompatibility and Mineralization Potential of Bio-C Sealer and TotalFill BC Sealer |
title_full | Comparative Cytocompatibility and Mineralization Potential of Bio-C Sealer and TotalFill BC Sealer |
title_fullStr | Comparative Cytocompatibility and Mineralization Potential of Bio-C Sealer and TotalFill BC Sealer |
title_full_unstemmed | Comparative Cytocompatibility and Mineralization Potential of Bio-C Sealer and TotalFill BC Sealer |
title_short | Comparative Cytocompatibility and Mineralization Potential of Bio-C Sealer and TotalFill BC Sealer |
title_sort | comparative cytocompatibility and mineralization potential of bio-c sealer and totalfill bc sealer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804055/ https://www.ncbi.nlm.nih.gov/pubmed/31546696 http://dx.doi.org/10.3390/ma12193087 |
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