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Residue of Paclobutrazol and Its Regulatory Effects on the Secondary Metabolites of Ophiopogon japonicas
Currently, paclobutrazol is excessively used in the planting process of Ophiopogon japonicus (O. japonicus) due to its important role in regulating the growth of tuber roots, ultimately increasing the yield and shortening the growth cycle of Ophiopogonis Radix. For insight into this process and the...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804066/ https://www.ncbi.nlm.nih.gov/pubmed/31569613 http://dx.doi.org/10.3390/molecules24193504 |
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author | Zhang, Lixia Luo, Zuliang Cui, Shengrong Xie, Lei Yu, Jing Tang, Deying Ma, Xiaojun Mou, Yan |
author_facet | Zhang, Lixia Luo, Zuliang Cui, Shengrong Xie, Lei Yu, Jing Tang, Deying Ma, Xiaojun Mou, Yan |
author_sort | Zhang, Lixia |
collection | PubMed |
description | Currently, paclobutrazol is excessively used in the planting process of Ophiopogon japonicus (O. japonicus) due to its important role in regulating the growth of tuber roots, ultimately increasing the yield and shortening the growth cycle of Ophiopogonis Radix. For insight into this process and the potential risks of paclobutrazol and its mediated consequences on the secondary metabolites in Ophiopogonis Radix, corresponding high performance liquid chromatography-tandem mass spectrometric methods (HPLC-MS/MS) were developed in this study and then applied to Ophiopogonis Radix, soil, and water samples. The results demonstrated the detection of different levels of paclobutrazol residue were in Ophiopogonis Radix, soil, and water samples. In addition, the quantitative results of the secondary metabolites showed that paclobutrazol significantly decreased four steroidal saponins in Ophiopogonis Radix, especially ophiopogonin D, where the content was decreased from 824.87 to 172.50 mg/kg. Concurrently, ophiopogonanone C, a flavonoid in Ophiopogonis Radix, also significantly decreased from 2.66 to 1.33 mg/kg. In conclusion, the residual paclobutrazol and its negative regulation on the secondary metabolism of Ophiopogonis Radix brings potential hazards to the environment and human health. These results provide more comprehensive data that can be used for the reassessment of the use of paclobutrazol in O. japonicus and the formulation of related standards. |
format | Online Article Text |
id | pubmed-6804066 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68040662019-11-18 Residue of Paclobutrazol and Its Regulatory Effects on the Secondary Metabolites of Ophiopogon japonicas Zhang, Lixia Luo, Zuliang Cui, Shengrong Xie, Lei Yu, Jing Tang, Deying Ma, Xiaojun Mou, Yan Molecules Article Currently, paclobutrazol is excessively used in the planting process of Ophiopogon japonicus (O. japonicus) due to its important role in regulating the growth of tuber roots, ultimately increasing the yield and shortening the growth cycle of Ophiopogonis Radix. For insight into this process and the potential risks of paclobutrazol and its mediated consequences on the secondary metabolites in Ophiopogonis Radix, corresponding high performance liquid chromatography-tandem mass spectrometric methods (HPLC-MS/MS) were developed in this study and then applied to Ophiopogonis Radix, soil, and water samples. The results demonstrated the detection of different levels of paclobutrazol residue were in Ophiopogonis Radix, soil, and water samples. In addition, the quantitative results of the secondary metabolites showed that paclobutrazol significantly decreased four steroidal saponins in Ophiopogonis Radix, especially ophiopogonin D, where the content was decreased from 824.87 to 172.50 mg/kg. Concurrently, ophiopogonanone C, a flavonoid in Ophiopogonis Radix, also significantly decreased from 2.66 to 1.33 mg/kg. In conclusion, the residual paclobutrazol and its negative regulation on the secondary metabolism of Ophiopogonis Radix brings potential hazards to the environment and human health. These results provide more comprehensive data that can be used for the reassessment of the use of paclobutrazol in O. japonicus and the formulation of related standards. MDPI 2019-09-27 /pmc/articles/PMC6804066/ /pubmed/31569613 http://dx.doi.org/10.3390/molecules24193504 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Lixia Luo, Zuliang Cui, Shengrong Xie, Lei Yu, Jing Tang, Deying Ma, Xiaojun Mou, Yan Residue of Paclobutrazol and Its Regulatory Effects on the Secondary Metabolites of Ophiopogon japonicas |
title | Residue of Paclobutrazol and Its Regulatory Effects on the Secondary Metabolites of Ophiopogon japonicas |
title_full | Residue of Paclobutrazol and Its Regulatory Effects on the Secondary Metabolites of Ophiopogon japonicas |
title_fullStr | Residue of Paclobutrazol and Its Regulatory Effects on the Secondary Metabolites of Ophiopogon japonicas |
title_full_unstemmed | Residue of Paclobutrazol and Its Regulatory Effects on the Secondary Metabolites of Ophiopogon japonicas |
title_short | Residue of Paclobutrazol and Its Regulatory Effects on the Secondary Metabolites of Ophiopogon japonicas |
title_sort | residue of paclobutrazol and its regulatory effects on the secondary metabolites of ophiopogon japonicas |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804066/ https://www.ncbi.nlm.nih.gov/pubmed/31569613 http://dx.doi.org/10.3390/molecules24193504 |
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