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Cell-Penetrating Peptide and Transferrin Co-Modified Liposomes for Targeted Therapy of Glioma
Glioma is one of the most aggressive and common malignant brain tumors. Due to the presence of the blood-brain barrier (BBB), the effectiveness of therapeutics is greatly affected. In this work, to develop an efficient anti-glioma drug with targeting and which was able to cross the BBB, cell-penetra...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804123/ https://www.ncbi.nlm.nih.gov/pubmed/31574945 http://dx.doi.org/10.3390/molecules24193540 |
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author | Wang, Xi Zhao, Yarong Dong, Shiyan Lee, Robert J. Yang, Dongsheng Zhang, Huan Teng, Lesheng |
author_facet | Wang, Xi Zhao, Yarong Dong, Shiyan Lee, Robert J. Yang, Dongsheng Zhang, Huan Teng, Lesheng |
author_sort | Wang, Xi |
collection | PubMed |
description | Glioma is one of the most aggressive and common malignant brain tumors. Due to the presence of the blood-brain barrier (BBB), the effectiveness of therapeutics is greatly affected. In this work, to develop an efficient anti-glioma drug with targeting and which was able to cross the BBB, cell-penetrating peptides (R8) and transferrin co-modified doxorubicin (DOX)-loaded liposomes (Tf-LPs) were prepared. Tf-LPs possessed a spherical shape and uniform size with 128.64 nm and their ξ-potential was 6.81 mV. Tf-LPs were found to be stable in serum within 48 h. Uptake of Tf-LPs in both U87 and GL261 cells was analyzed by confocal laser scanning microscopy and by flow cytometry. Tf-LPs were efficiently taken up by both U87 and GL261 cells. Moreover, Tf-LPs exhibited sustained-release. The cumulative release of DOX from Tf-LPs reached ~50.0% and showed excellent anti-glioma efficacy. Histology of major organs, including brain, heart, liver, spleen, lungs and kidney, and the bodyweight of mice, all indicated low toxicity of Tf-LPs. In conclusion, Tf-LPs showed great promise as an anti-glioma therapeutic agent. |
format | Online Article Text |
id | pubmed-6804123 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68041232019-11-18 Cell-Penetrating Peptide and Transferrin Co-Modified Liposomes for Targeted Therapy of Glioma Wang, Xi Zhao, Yarong Dong, Shiyan Lee, Robert J. Yang, Dongsheng Zhang, Huan Teng, Lesheng Molecules Article Glioma is one of the most aggressive and common malignant brain tumors. Due to the presence of the blood-brain barrier (BBB), the effectiveness of therapeutics is greatly affected. In this work, to develop an efficient anti-glioma drug with targeting and which was able to cross the BBB, cell-penetrating peptides (R8) and transferrin co-modified doxorubicin (DOX)-loaded liposomes (Tf-LPs) were prepared. Tf-LPs possessed a spherical shape and uniform size with 128.64 nm and their ξ-potential was 6.81 mV. Tf-LPs were found to be stable in serum within 48 h. Uptake of Tf-LPs in both U87 and GL261 cells was analyzed by confocal laser scanning microscopy and by flow cytometry. Tf-LPs were efficiently taken up by both U87 and GL261 cells. Moreover, Tf-LPs exhibited sustained-release. The cumulative release of DOX from Tf-LPs reached ~50.0% and showed excellent anti-glioma efficacy. Histology of major organs, including brain, heart, liver, spleen, lungs and kidney, and the bodyweight of mice, all indicated low toxicity of Tf-LPs. In conclusion, Tf-LPs showed great promise as an anti-glioma therapeutic agent. MDPI 2019-09-30 /pmc/articles/PMC6804123/ /pubmed/31574945 http://dx.doi.org/10.3390/molecules24193540 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Xi Zhao, Yarong Dong, Shiyan Lee, Robert J. Yang, Dongsheng Zhang, Huan Teng, Lesheng Cell-Penetrating Peptide and Transferrin Co-Modified Liposomes for Targeted Therapy of Glioma |
title | Cell-Penetrating Peptide and Transferrin Co-Modified Liposomes for Targeted Therapy of Glioma |
title_full | Cell-Penetrating Peptide and Transferrin Co-Modified Liposomes for Targeted Therapy of Glioma |
title_fullStr | Cell-Penetrating Peptide and Transferrin Co-Modified Liposomes for Targeted Therapy of Glioma |
title_full_unstemmed | Cell-Penetrating Peptide and Transferrin Co-Modified Liposomes for Targeted Therapy of Glioma |
title_short | Cell-Penetrating Peptide and Transferrin Co-Modified Liposomes for Targeted Therapy of Glioma |
title_sort | cell-penetrating peptide and transferrin co-modified liposomes for targeted therapy of glioma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804123/ https://www.ncbi.nlm.nih.gov/pubmed/31574945 http://dx.doi.org/10.3390/molecules24193540 |
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