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Identification of Chemical Markers for the Discrimination of Radix Angelica sinensis Grown in Geoherb and Non-Geoherb Regions Using UHPLC-QTOF-MS/MS Based Metabolomics

This research aimed to discover chemical markers for discriminating radix Angelica sinensis (RAS) from different regions and to explore the differences of RAS in the content of four active compounds and anti-inflammatory activities on lipopolysacchride (LPS)-induced RAW264.7 cells and calcium antago...

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Autores principales: Zhang, Kaixue, Yan, Menglin, Han, Shu, Cong, Longfei, Wang, Liyao, Zhang, Liu, Sun, Lili, Bai, Haiying, Wei, Guanhua, Du, Hong, Jiang, Min, Bai, Gang, Yang, Zhigang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804141/
https://www.ncbi.nlm.nih.gov/pubmed/31574916
http://dx.doi.org/10.3390/molecules24193536
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author Zhang, Kaixue
Yan, Menglin
Han, Shu
Cong, Longfei
Wang, Liyao
Zhang, Liu
Sun, Lili
Bai, Haiying
Wei, Guanhua
Du, Hong
Jiang, Min
Bai, Gang
Yang, Zhigang
author_facet Zhang, Kaixue
Yan, Menglin
Han, Shu
Cong, Longfei
Wang, Liyao
Zhang, Liu
Sun, Lili
Bai, Haiying
Wei, Guanhua
Du, Hong
Jiang, Min
Bai, Gang
Yang, Zhigang
author_sort Zhang, Kaixue
collection PubMed
description This research aimed to discover chemical markers for discriminating radix Angelica sinensis (RAS) from different regions and to explore the differences of RAS in the content of four active compounds and anti-inflammatory activities on lipopolysacchride (LPS)-induced RAW264.7 cells and calcium antagonists on the HEK 293T cells of RAS. Nine compounds were selected as characteristic chemical markers by ultra-high-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry (UHPLC-QTOF-MS/MS), based on metabolomics, in order to rapidly discriminate RAS from geoherb and non-geoherb regions. The contents of senkyunolide I and butylidenephthalide in geoherb samples were higher than those in non-geoherb samples, but the contents of ferulic acid and levistolide A were lower in the geoherb samples. Furthermore, the geoherbs showed better nitric oxide (NO) inhibitory and calcium antagonistic activities than the non-geoherbs. These results demonstrate the diversity in quality of RAS between geoherbs and non-geoherbs.
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spelling pubmed-68041412019-11-18 Identification of Chemical Markers for the Discrimination of Radix Angelica sinensis Grown in Geoherb and Non-Geoherb Regions Using UHPLC-QTOF-MS/MS Based Metabolomics Zhang, Kaixue Yan, Menglin Han, Shu Cong, Longfei Wang, Liyao Zhang, Liu Sun, Lili Bai, Haiying Wei, Guanhua Du, Hong Jiang, Min Bai, Gang Yang, Zhigang Molecules Article This research aimed to discover chemical markers for discriminating radix Angelica sinensis (RAS) from different regions and to explore the differences of RAS in the content of four active compounds and anti-inflammatory activities on lipopolysacchride (LPS)-induced RAW264.7 cells and calcium antagonists on the HEK 293T cells of RAS. Nine compounds were selected as characteristic chemical markers by ultra-high-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry (UHPLC-QTOF-MS/MS), based on metabolomics, in order to rapidly discriminate RAS from geoherb and non-geoherb regions. The contents of senkyunolide I and butylidenephthalide in geoherb samples were higher than those in non-geoherb samples, but the contents of ferulic acid and levistolide A were lower in the geoherb samples. Furthermore, the geoherbs showed better nitric oxide (NO) inhibitory and calcium antagonistic activities than the non-geoherbs. These results demonstrate the diversity in quality of RAS between geoherbs and non-geoherbs. MDPI 2019-09-30 /pmc/articles/PMC6804141/ /pubmed/31574916 http://dx.doi.org/10.3390/molecules24193536 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Kaixue
Yan, Menglin
Han, Shu
Cong, Longfei
Wang, Liyao
Zhang, Liu
Sun, Lili
Bai, Haiying
Wei, Guanhua
Du, Hong
Jiang, Min
Bai, Gang
Yang, Zhigang
Identification of Chemical Markers for the Discrimination of Radix Angelica sinensis Grown in Geoherb and Non-Geoherb Regions Using UHPLC-QTOF-MS/MS Based Metabolomics
title Identification of Chemical Markers for the Discrimination of Radix Angelica sinensis Grown in Geoherb and Non-Geoherb Regions Using UHPLC-QTOF-MS/MS Based Metabolomics
title_full Identification of Chemical Markers for the Discrimination of Radix Angelica sinensis Grown in Geoherb and Non-Geoherb Regions Using UHPLC-QTOF-MS/MS Based Metabolomics
title_fullStr Identification of Chemical Markers for the Discrimination of Radix Angelica sinensis Grown in Geoherb and Non-Geoherb Regions Using UHPLC-QTOF-MS/MS Based Metabolomics
title_full_unstemmed Identification of Chemical Markers for the Discrimination of Radix Angelica sinensis Grown in Geoherb and Non-Geoherb Regions Using UHPLC-QTOF-MS/MS Based Metabolomics
title_short Identification of Chemical Markers for the Discrimination of Radix Angelica sinensis Grown in Geoherb and Non-Geoherb Regions Using UHPLC-QTOF-MS/MS Based Metabolomics
title_sort identification of chemical markers for the discrimination of radix angelica sinensis grown in geoherb and non-geoherb regions using uhplc-qtof-ms/ms based metabolomics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804141/
https://www.ncbi.nlm.nih.gov/pubmed/31574916
http://dx.doi.org/10.3390/molecules24193536
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