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Macromolecular Nanocrystal Structural Analysis with Electron and X-Rays: A Comparative Review

Crystallography has long been the unrivaled method that can provide the atomistic structural models of macromolecules, using either X-rays or electrons as probes. The methodology has gone through several revolutionary periods, driven by the development of new sources, detectors, and other instrument...

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Detalles Bibliográficos
Autores principales: Khakurel, Krishna P., Angelov, Borislav, Andreasson, Jakob
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804143/
https://www.ncbi.nlm.nih.gov/pubmed/31561479
http://dx.doi.org/10.3390/molecules24193490
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author Khakurel, Krishna P.
Angelov, Borislav
Andreasson, Jakob
author_facet Khakurel, Krishna P.
Angelov, Borislav
Andreasson, Jakob
author_sort Khakurel, Krishna P.
collection PubMed
description Crystallography has long been the unrivaled method that can provide the atomistic structural models of macromolecules, using either X-rays or electrons as probes. The methodology has gone through several revolutionary periods, driven by the development of new sources, detectors, and other instrumentation. Novel sources of both X-ray and electrons are constantly emerging. The increase in brightness of these sources, complemented by the advanced detection techniques, has relaxed the traditionally strict need for large, high quality, crystals. Recent reports suggest high-quality diffraction datasets from crystals as small as a few hundreds of nanometers can be routinely obtained. This has resulted in the genesis of a new field of macromolecular nanocrystal crystallography. Here we will make a brief comparative review of this growing field focusing on the use of X-rays and electrons sources.
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spelling pubmed-68041432019-11-18 Macromolecular Nanocrystal Structural Analysis with Electron and X-Rays: A Comparative Review Khakurel, Krishna P. Angelov, Borislav Andreasson, Jakob Molecules Review Crystallography has long been the unrivaled method that can provide the atomistic structural models of macromolecules, using either X-rays or electrons as probes. The methodology has gone through several revolutionary periods, driven by the development of new sources, detectors, and other instrumentation. Novel sources of both X-ray and electrons are constantly emerging. The increase in brightness of these sources, complemented by the advanced detection techniques, has relaxed the traditionally strict need for large, high quality, crystals. Recent reports suggest high-quality diffraction datasets from crystals as small as a few hundreds of nanometers can be routinely obtained. This has resulted in the genesis of a new field of macromolecular nanocrystal crystallography. Here we will make a brief comparative review of this growing field focusing on the use of X-rays and electrons sources. MDPI 2019-09-26 /pmc/articles/PMC6804143/ /pubmed/31561479 http://dx.doi.org/10.3390/molecules24193490 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Khakurel, Krishna P.
Angelov, Borislav
Andreasson, Jakob
Macromolecular Nanocrystal Structural Analysis with Electron and X-Rays: A Comparative Review
title Macromolecular Nanocrystal Structural Analysis with Electron and X-Rays: A Comparative Review
title_full Macromolecular Nanocrystal Structural Analysis with Electron and X-Rays: A Comparative Review
title_fullStr Macromolecular Nanocrystal Structural Analysis with Electron and X-Rays: A Comparative Review
title_full_unstemmed Macromolecular Nanocrystal Structural Analysis with Electron and X-Rays: A Comparative Review
title_short Macromolecular Nanocrystal Structural Analysis with Electron and X-Rays: A Comparative Review
title_sort macromolecular nanocrystal structural analysis with electron and x-rays: a comparative review
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804143/
https://www.ncbi.nlm.nih.gov/pubmed/31561479
http://dx.doi.org/10.3390/molecules24193490
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