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Considerations when Measuring Biocatalyst Performance

As biocatalysis matures, it becomes increasingly important to establish methods with which to measure biocatalyst performance. Such measurements are important to assess immobilization strategies, different operating modes, and reactor configurations, aside from comparing protein engineered variants...

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Detalles Bibliográficos
Autores principales: Dias Gomes, Mafalda, Woodley, John M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804192/
https://www.ncbi.nlm.nih.gov/pubmed/31623317
http://dx.doi.org/10.3390/molecules24193573
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author Dias Gomes, Mafalda
Woodley, John M.
author_facet Dias Gomes, Mafalda
Woodley, John M.
author_sort Dias Gomes, Mafalda
collection PubMed
description As biocatalysis matures, it becomes increasingly important to establish methods with which to measure biocatalyst performance. Such measurements are important to assess immobilization strategies, different operating modes, and reactor configurations, aside from comparing protein engineered variants and benchmarking against economic targets. While conventional measurement techniques focus on a single performance metric (such as the total turnover number), here, it is argued that three metrics (achievable product concentration, productivity, and enzyme stability) are required for an accurate assessment of scalability.
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spelling pubmed-68041922019-11-18 Considerations when Measuring Biocatalyst Performance Dias Gomes, Mafalda Woodley, John M. Molecules Concept Paper As biocatalysis matures, it becomes increasingly important to establish methods with which to measure biocatalyst performance. Such measurements are important to assess immobilization strategies, different operating modes, and reactor configurations, aside from comparing protein engineered variants and benchmarking against economic targets. While conventional measurement techniques focus on a single performance metric (such as the total turnover number), here, it is argued that three metrics (achievable product concentration, productivity, and enzyme stability) are required for an accurate assessment of scalability. MDPI 2019-10-03 /pmc/articles/PMC6804192/ /pubmed/31623317 http://dx.doi.org/10.3390/molecules24193573 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Concept Paper
Dias Gomes, Mafalda
Woodley, John M.
Considerations when Measuring Biocatalyst Performance
title Considerations when Measuring Biocatalyst Performance
title_full Considerations when Measuring Biocatalyst Performance
title_fullStr Considerations when Measuring Biocatalyst Performance
title_full_unstemmed Considerations when Measuring Biocatalyst Performance
title_short Considerations when Measuring Biocatalyst Performance
title_sort considerations when measuring biocatalyst performance
topic Concept Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804192/
https://www.ncbi.nlm.nih.gov/pubmed/31623317
http://dx.doi.org/10.3390/molecules24193573
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