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Multi-Evaluating Strategy for Siji-kangbingdu Mixture: Chemical Profiling, Fingerprint Characterization, and Quantitative Analysis

Siji-kangbingdu mixture is an anti-inflammatory, anti-bacterial, and anti-viral herbal mixture which is frequently used by doctors to treat upper respiratory infections. It’s important to establish an efficient and economical quality-control method to ensure the quality consistency and efficacy stab...

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Autores principales: Yao, Zhuoru, Yu, Jingao, Tang, Zhishu, Liu, Hongbo, Ruan, Kaihua, Song, Zhongxing, Liu, Yanru, Yan, Kun, Liu, Yan, Tang, Yuping, Ma, Huqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804210/
https://www.ncbi.nlm.nih.gov/pubmed/31574981
http://dx.doi.org/10.3390/molecules24193545
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author Yao, Zhuoru
Yu, Jingao
Tang, Zhishu
Liu, Hongbo
Ruan, Kaihua
Song, Zhongxing
Liu, Yanru
Yan, Kun
Liu, Yan
Tang, Yuping
Ma, Huqiang
author_facet Yao, Zhuoru
Yu, Jingao
Tang, Zhishu
Liu, Hongbo
Ruan, Kaihua
Song, Zhongxing
Liu, Yanru
Yan, Kun
Liu, Yan
Tang, Yuping
Ma, Huqiang
author_sort Yao, Zhuoru
collection PubMed
description Siji-kangbingdu mixture is an anti-inflammatory, anti-bacterial, and anti-viral herbal mixture which is frequently used by doctors to treat upper respiratory infections. It’s important to establish an efficient and economical quality-control method to ensure the quality consistency and efficacy stability of Siji-kangbingdu mixture. In this study, an integrated multi-evaluation method was established, sequentially involving UPLC-TripleTOF-MS analysis, UPLC fingerprint analysis, and the quantitative analysis of multi-components using the single-marker (QAMS) method. With one chromatographic condition, a total of 71 compounds were identified by MS and MS/MS information, with a mass error of less than 5 ppm; 49 peaks detected in 254 nm were selected to establish the fingerprint similarity model, and 7 chemical compounds were simultaneously determined, namely, chlorogenic acid, liquiritin, rutin, isochlorogenic acid A, forsythin, forsythoside A, and glycyrrhizic acid, with forsythoside A as the reference standard. There was no significant difference in the content of the seven compounds between the QAMS method and the external standard method (ESM). The established multi-evaluation method will largely promote the quality control and standardization process of Siji-kangbingdu mixture. It also provides a reference workflow for the overall evaluation of TCM patent medicines, from chemical profiling to fingerprint and quantitative analysis.
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spelling pubmed-68042102019-11-18 Multi-Evaluating Strategy for Siji-kangbingdu Mixture: Chemical Profiling, Fingerprint Characterization, and Quantitative Analysis Yao, Zhuoru Yu, Jingao Tang, Zhishu Liu, Hongbo Ruan, Kaihua Song, Zhongxing Liu, Yanru Yan, Kun Liu, Yan Tang, Yuping Ma, Huqiang Molecules Article Siji-kangbingdu mixture is an anti-inflammatory, anti-bacterial, and anti-viral herbal mixture which is frequently used by doctors to treat upper respiratory infections. It’s important to establish an efficient and economical quality-control method to ensure the quality consistency and efficacy stability of Siji-kangbingdu mixture. In this study, an integrated multi-evaluation method was established, sequentially involving UPLC-TripleTOF-MS analysis, UPLC fingerprint analysis, and the quantitative analysis of multi-components using the single-marker (QAMS) method. With one chromatographic condition, a total of 71 compounds were identified by MS and MS/MS information, with a mass error of less than 5 ppm; 49 peaks detected in 254 nm were selected to establish the fingerprint similarity model, and 7 chemical compounds were simultaneously determined, namely, chlorogenic acid, liquiritin, rutin, isochlorogenic acid A, forsythin, forsythoside A, and glycyrrhizic acid, with forsythoside A as the reference standard. There was no significant difference in the content of the seven compounds between the QAMS method and the external standard method (ESM). The established multi-evaluation method will largely promote the quality control and standardization process of Siji-kangbingdu mixture. It also provides a reference workflow for the overall evaluation of TCM patent medicines, from chemical profiling to fingerprint and quantitative analysis. MDPI 2019-09-30 /pmc/articles/PMC6804210/ /pubmed/31574981 http://dx.doi.org/10.3390/molecules24193545 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yao, Zhuoru
Yu, Jingao
Tang, Zhishu
Liu, Hongbo
Ruan, Kaihua
Song, Zhongxing
Liu, Yanru
Yan, Kun
Liu, Yan
Tang, Yuping
Ma, Huqiang
Multi-Evaluating Strategy for Siji-kangbingdu Mixture: Chemical Profiling, Fingerprint Characterization, and Quantitative Analysis
title Multi-Evaluating Strategy for Siji-kangbingdu Mixture: Chemical Profiling, Fingerprint Characterization, and Quantitative Analysis
title_full Multi-Evaluating Strategy for Siji-kangbingdu Mixture: Chemical Profiling, Fingerprint Characterization, and Quantitative Analysis
title_fullStr Multi-Evaluating Strategy for Siji-kangbingdu Mixture: Chemical Profiling, Fingerprint Characterization, and Quantitative Analysis
title_full_unstemmed Multi-Evaluating Strategy for Siji-kangbingdu Mixture: Chemical Profiling, Fingerprint Characterization, and Quantitative Analysis
title_short Multi-Evaluating Strategy for Siji-kangbingdu Mixture: Chemical Profiling, Fingerprint Characterization, and Quantitative Analysis
title_sort multi-evaluating strategy for siji-kangbingdu mixture: chemical profiling, fingerprint characterization, and quantitative analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804210/
https://www.ncbi.nlm.nih.gov/pubmed/31574981
http://dx.doi.org/10.3390/molecules24193545
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