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Biological Effects of Shikonin in Human Gingival Fibroblasts via ERK 1/2 Signaling Pathway
Shikonin, an active ingredient of Lithospermum erythrorhizon, exerts anti-inflammatory and antibacterial effects, and promotes wound healing. We investigated whether shikonin stimulated gingival tissue wound healing in human gingival fibroblasts (hGF). In addition, we evaluated the effects of shikon...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804247/ https://www.ncbi.nlm.nih.gov/pubmed/31574951 http://dx.doi.org/10.3390/molecules24193542 |
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author | Imai, Kazutaka Kato, Hirohito Taguchi, Yoichiro Umeda, Makoto |
author_facet | Imai, Kazutaka Kato, Hirohito Taguchi, Yoichiro Umeda, Makoto |
author_sort | Imai, Kazutaka |
collection | PubMed |
description | Shikonin, an active ingredient of Lithospermum erythrorhizon, exerts anti-inflammatory and antibacterial effects, and promotes wound healing. We investigated whether shikonin stimulated gingival tissue wound healing in human gingival fibroblasts (hGF). In addition, we evaluated the effects of shikonin on the mitogen-activated protein kinase (MAPK) signaling pathway, which has an important role in wound healing. hGF were subjected to primary culture using gingiva collected from patients. The cells were exposed to/treated with Shikonin at concentrations ranging from 0.01 to 100 μM. The optimal concentration was determined by cell proliferation and migration assays. Type I collagen and fibronectin synthesis, the gene expression of vascular endothelial growth factor (VEGF) and FN, and the phosphorylation of Extracellular signal-regulated kinase (ERK) 1/2 were investigated. Identical experiments were performed in the presence of PD98059 our data suggest, a specific ERK 1/2 inhibitor. Shikonin significantly promoted hGF proliferation and migration. Shikonin (1 µM) was chosen as the optimal concentration. Shikonin promoted type I collagen and FN synthesis, increased VEGF and FN expression, and induced ERK 1/2 phosphorylation. These changes were partially suppressed by PD98059. In conclusion, Shikonin promoted the proliferation, migration, type I collagen and FN synthesis, and expression of VEGF and FN via ERK 1/2 signaling pathway in hGFs. Therefore, shikonin may promote periodontal tissue wound healing. |
format | Online Article Text |
id | pubmed-6804247 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68042472019-11-18 Biological Effects of Shikonin in Human Gingival Fibroblasts via ERK 1/2 Signaling Pathway Imai, Kazutaka Kato, Hirohito Taguchi, Yoichiro Umeda, Makoto Molecules Article Shikonin, an active ingredient of Lithospermum erythrorhizon, exerts anti-inflammatory and antibacterial effects, and promotes wound healing. We investigated whether shikonin stimulated gingival tissue wound healing in human gingival fibroblasts (hGF). In addition, we evaluated the effects of shikonin on the mitogen-activated protein kinase (MAPK) signaling pathway, which has an important role in wound healing. hGF were subjected to primary culture using gingiva collected from patients. The cells were exposed to/treated with Shikonin at concentrations ranging from 0.01 to 100 μM. The optimal concentration was determined by cell proliferation and migration assays. Type I collagen and fibronectin synthesis, the gene expression of vascular endothelial growth factor (VEGF) and FN, and the phosphorylation of Extracellular signal-regulated kinase (ERK) 1/2 were investigated. Identical experiments were performed in the presence of PD98059 our data suggest, a specific ERK 1/2 inhibitor. Shikonin significantly promoted hGF proliferation and migration. Shikonin (1 µM) was chosen as the optimal concentration. Shikonin promoted type I collagen and FN synthesis, increased VEGF and FN expression, and induced ERK 1/2 phosphorylation. These changes were partially suppressed by PD98059. In conclusion, Shikonin promoted the proliferation, migration, type I collagen and FN synthesis, and expression of VEGF and FN via ERK 1/2 signaling pathway in hGFs. Therefore, shikonin may promote periodontal tissue wound healing. MDPI 2019-09-30 /pmc/articles/PMC6804247/ /pubmed/31574951 http://dx.doi.org/10.3390/molecules24193542 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Imai, Kazutaka Kato, Hirohito Taguchi, Yoichiro Umeda, Makoto Biological Effects of Shikonin in Human Gingival Fibroblasts via ERK 1/2 Signaling Pathway |
title | Biological Effects of Shikonin in Human Gingival Fibroblasts via ERK 1/2 Signaling Pathway |
title_full | Biological Effects of Shikonin in Human Gingival Fibroblasts via ERK 1/2 Signaling Pathway |
title_fullStr | Biological Effects of Shikonin in Human Gingival Fibroblasts via ERK 1/2 Signaling Pathway |
title_full_unstemmed | Biological Effects of Shikonin in Human Gingival Fibroblasts via ERK 1/2 Signaling Pathway |
title_short | Biological Effects of Shikonin in Human Gingival Fibroblasts via ERK 1/2 Signaling Pathway |
title_sort | biological effects of shikonin in human gingival fibroblasts via erk 1/2 signaling pathway |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804247/ https://www.ncbi.nlm.nih.gov/pubmed/31574951 http://dx.doi.org/10.3390/molecules24193542 |
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