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Biological Effects of Shikonin in Human Gingival Fibroblasts via ERK 1/2 Signaling Pathway

Shikonin, an active ingredient of Lithospermum erythrorhizon, exerts anti-inflammatory and antibacterial effects, and promotes wound healing. We investigated whether shikonin stimulated gingival tissue wound healing in human gingival fibroblasts (hGF). In addition, we evaluated the effects of shikon...

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Autores principales: Imai, Kazutaka, Kato, Hirohito, Taguchi, Yoichiro, Umeda, Makoto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804247/
https://www.ncbi.nlm.nih.gov/pubmed/31574951
http://dx.doi.org/10.3390/molecules24193542
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author Imai, Kazutaka
Kato, Hirohito
Taguchi, Yoichiro
Umeda, Makoto
author_facet Imai, Kazutaka
Kato, Hirohito
Taguchi, Yoichiro
Umeda, Makoto
author_sort Imai, Kazutaka
collection PubMed
description Shikonin, an active ingredient of Lithospermum erythrorhizon, exerts anti-inflammatory and antibacterial effects, and promotes wound healing. We investigated whether shikonin stimulated gingival tissue wound healing in human gingival fibroblasts (hGF). In addition, we evaluated the effects of shikonin on the mitogen-activated protein kinase (MAPK) signaling pathway, which has an important role in wound healing. hGF were subjected to primary culture using gingiva collected from patients. The cells were exposed to/treated with Shikonin at concentrations ranging from 0.01 to 100 μM. The optimal concentration was determined by cell proliferation and migration assays. Type I collagen and fibronectin synthesis, the gene expression of vascular endothelial growth factor (VEGF) and FN, and the phosphorylation of Extracellular signal-regulated kinase (ERK) 1/2 were investigated. Identical experiments were performed in the presence of PD98059 our data suggest, a specific ERK 1/2 inhibitor. Shikonin significantly promoted hGF proliferation and migration. Shikonin (1 µM) was chosen as the optimal concentration. Shikonin promoted type I collagen and FN synthesis, increased VEGF and FN expression, and induced ERK 1/2 phosphorylation. These changes were partially suppressed by PD98059. In conclusion, Shikonin promoted the proliferation, migration, type I collagen and FN synthesis, and expression of VEGF and FN via ERK 1/2 signaling pathway in hGFs. Therefore, shikonin may promote periodontal tissue wound healing.
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spelling pubmed-68042472019-11-18 Biological Effects of Shikonin in Human Gingival Fibroblasts via ERK 1/2 Signaling Pathway Imai, Kazutaka Kato, Hirohito Taguchi, Yoichiro Umeda, Makoto Molecules Article Shikonin, an active ingredient of Lithospermum erythrorhizon, exerts anti-inflammatory and antibacterial effects, and promotes wound healing. We investigated whether shikonin stimulated gingival tissue wound healing in human gingival fibroblasts (hGF). In addition, we evaluated the effects of shikonin on the mitogen-activated protein kinase (MAPK) signaling pathway, which has an important role in wound healing. hGF were subjected to primary culture using gingiva collected from patients. The cells were exposed to/treated with Shikonin at concentrations ranging from 0.01 to 100 μM. The optimal concentration was determined by cell proliferation and migration assays. Type I collagen and fibronectin synthesis, the gene expression of vascular endothelial growth factor (VEGF) and FN, and the phosphorylation of Extracellular signal-regulated kinase (ERK) 1/2 were investigated. Identical experiments were performed in the presence of PD98059 our data suggest, a specific ERK 1/2 inhibitor. Shikonin significantly promoted hGF proliferation and migration. Shikonin (1 µM) was chosen as the optimal concentration. Shikonin promoted type I collagen and FN synthesis, increased VEGF and FN expression, and induced ERK 1/2 phosphorylation. These changes were partially suppressed by PD98059. In conclusion, Shikonin promoted the proliferation, migration, type I collagen and FN synthesis, and expression of VEGF and FN via ERK 1/2 signaling pathway in hGFs. Therefore, shikonin may promote periodontal tissue wound healing. MDPI 2019-09-30 /pmc/articles/PMC6804247/ /pubmed/31574951 http://dx.doi.org/10.3390/molecules24193542 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Imai, Kazutaka
Kato, Hirohito
Taguchi, Yoichiro
Umeda, Makoto
Biological Effects of Shikonin in Human Gingival Fibroblasts via ERK 1/2 Signaling Pathway
title Biological Effects of Shikonin in Human Gingival Fibroblasts via ERK 1/2 Signaling Pathway
title_full Biological Effects of Shikonin in Human Gingival Fibroblasts via ERK 1/2 Signaling Pathway
title_fullStr Biological Effects of Shikonin in Human Gingival Fibroblasts via ERK 1/2 Signaling Pathway
title_full_unstemmed Biological Effects of Shikonin in Human Gingival Fibroblasts via ERK 1/2 Signaling Pathway
title_short Biological Effects of Shikonin in Human Gingival Fibroblasts via ERK 1/2 Signaling Pathway
title_sort biological effects of shikonin in human gingival fibroblasts via erk 1/2 signaling pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804247/
https://www.ncbi.nlm.nih.gov/pubmed/31574951
http://dx.doi.org/10.3390/molecules24193542
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