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(99m)Tc-3PRGD(2) SPECT Predicts the Outcome of Endostar and Cisplatin Therapy in Xenograft Animals
AIMS: Our study was designed to investigate the usefulness of (99m)Tc-3PRGD(2) single-photon emission computed tomography (SPECT) for noninvasively monitoring the response of integrin α(v)β(3) expression to antiangiogenic treatment with endostar and cisplatin in xenograft animals. METHODS: (99m)Tc-3...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804356/ https://www.ncbi.nlm.nih.gov/pubmed/31673250 http://dx.doi.org/10.1177/1559325819882544 |
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author | Sun, Wei He, Guifu Zhang, Mingming Zhao, Yi Yu, Hongmei Li, Yi Wu, Wei Ji, Tiefeng |
author_facet | Sun, Wei He, Guifu Zhang, Mingming Zhao, Yi Yu, Hongmei Li, Yi Wu, Wei Ji, Tiefeng |
author_sort | Sun, Wei |
collection | PubMed |
description | AIMS: Our study was designed to investigate the usefulness of (99m)Tc-3PRGD(2) single-photon emission computed tomography (SPECT) for noninvasively monitoring the response of integrin α(v)β(3) expression to antiangiogenic treatment with endostar and cisplatin in xenograft animals. METHODS: (99m)Tc-3PRGD(2) SPECT imaging was performed at days 0, 7, 14, and 21. Tumors were harvested at all imaging time points for Western blotting and histopathological analysis. RESULT: In (99m)Tc-3PRGD(2) SPECT imaging, the radioactivity accumulation of NaCl group rised gradually in the first half and dispersed on day 21 due to the necrosis of the tumor. While the radioactivity accumulation of treated groups gradually decreased throughout the course. The downtrend of tumor to nontumor ratio in endostar-treated group was more remarkable than cisplatin-treated group. The expression of intergrin α(v)β(3) of treated groups was lower than NaCl group from day 14. The expression of intergrin α(v)β(3) of endostar-treated group was significantly lower than cisplatin-treated group from baseline onward. CONCLUSION: It’s demonstrated that the (99m)Tc-3PRGD(2) could noninvasively visualize and semiquantify tumor angiogenesis in the xenograft model and monitor the response to the antiangiogenic therapy of endostar and cisplatin effectively. It also can predict the outcome of endostar and cisplatin therapy in xenograft animals. |
format | Online Article Text |
id | pubmed-6804356 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-68043562019-10-31 (99m)Tc-3PRGD(2) SPECT Predicts the Outcome of Endostar and Cisplatin Therapy in Xenograft Animals Sun, Wei He, Guifu Zhang, Mingming Zhao, Yi Yu, Hongmei Li, Yi Wu, Wei Ji, Tiefeng Dose Response Original Article AIMS: Our study was designed to investigate the usefulness of (99m)Tc-3PRGD(2) single-photon emission computed tomography (SPECT) for noninvasively monitoring the response of integrin α(v)β(3) expression to antiangiogenic treatment with endostar and cisplatin in xenograft animals. METHODS: (99m)Tc-3PRGD(2) SPECT imaging was performed at days 0, 7, 14, and 21. Tumors were harvested at all imaging time points for Western blotting and histopathological analysis. RESULT: In (99m)Tc-3PRGD(2) SPECT imaging, the radioactivity accumulation of NaCl group rised gradually in the first half and dispersed on day 21 due to the necrosis of the tumor. While the radioactivity accumulation of treated groups gradually decreased throughout the course. The downtrend of tumor to nontumor ratio in endostar-treated group was more remarkable than cisplatin-treated group. The expression of intergrin α(v)β(3) of treated groups was lower than NaCl group from day 14. The expression of intergrin α(v)β(3) of endostar-treated group was significantly lower than cisplatin-treated group from baseline onward. CONCLUSION: It’s demonstrated that the (99m)Tc-3PRGD(2) could noninvasively visualize and semiquantify tumor angiogenesis in the xenograft model and monitor the response to the antiangiogenic therapy of endostar and cisplatin effectively. It also can predict the outcome of endostar and cisplatin therapy in xenograft animals. SAGE Publications 2019-10-20 /pmc/articles/PMC6804356/ /pubmed/31673250 http://dx.doi.org/10.1177/1559325819882544 Text en © The Author(s) 2019 http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Article Sun, Wei He, Guifu Zhang, Mingming Zhao, Yi Yu, Hongmei Li, Yi Wu, Wei Ji, Tiefeng (99m)Tc-3PRGD(2) SPECT Predicts the Outcome of Endostar and Cisplatin Therapy in Xenograft Animals |
title |
(99m)Tc-3PRGD(2) SPECT Predicts the Outcome of Endostar and Cisplatin Therapy in Xenograft Animals |
title_full |
(99m)Tc-3PRGD(2) SPECT Predicts the Outcome of Endostar and Cisplatin Therapy in Xenograft Animals |
title_fullStr |
(99m)Tc-3PRGD(2) SPECT Predicts the Outcome of Endostar and Cisplatin Therapy in Xenograft Animals |
title_full_unstemmed |
(99m)Tc-3PRGD(2) SPECT Predicts the Outcome of Endostar and Cisplatin Therapy in Xenograft Animals |
title_short |
(99m)Tc-3PRGD(2) SPECT Predicts the Outcome of Endostar and Cisplatin Therapy in Xenograft Animals |
title_sort | (99m)tc-3prgd(2) spect predicts the outcome of endostar and cisplatin therapy in xenograft animals |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804356/ https://www.ncbi.nlm.nih.gov/pubmed/31673250 http://dx.doi.org/10.1177/1559325819882544 |
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